FastQCFastQC Report
Mon 24 Feb 2025
POR-262-TP3_R2_001.fastp-trim.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamePOR-262-TP3_R2_001.fastp-trim.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21153661
Total Bases2.8 Gbp
Sequences flagged as poor quality0
Sequence length15-135
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTAGGGTGCTTGAAACCCAGAGGCAGAATGAAAGTGAAGACGGCTTC1749840.8272043312030007No Hit
GAACTTTGAAGACCGAAGTGGAGAAAGGTTCCATGTGAACAGCAGTTGGA983160.46477061346496945No Hit
GAGTCTTAGGGTGCTTGAAACCCAGAGGCAGAATGAAAGTGAAGACGGCT929590.43944639180896394No Hit
GACGAACTACTGCGAAAGCATTTGCCAAGAATGTTTTCATTAATCAAGAA808920.38240189251401924No Hit
CTTTCTTGATTCTATGGGTGGTGGTGCATGGCCGTTCTTAGTTGGTGGAG729880.34503720183470843No Hit
GCTTGAAACCCAGAGGCAGAATGAAAGTGAAGACGGCTTCGGCCGCTGAG712490.3368164026075675No Hit
TGAGAGCTCTTTCTTGATTCTATGGGTGGTGGTGCATGGCCGTTCTTAGT619780.2929894735478648No Hit
CAGAATGAAAGTGAAGACGGCTTCGGCCGCTGAGGCGAGATTCCGACTCT605010.2860072306160149No Hit
GTCTCAAAGATTAAGCCATGCATGTCTAAGTATAAGCACTAGTACTGTGA597990.28268865611489186No Hit
TGAGGATTGACAGATTGAGAGCTCTTTCTTGATTCTATGGGTGGTGGTGC574000.2713478295790029No Hit
AAAGTACGTGAAACCGTTTGAAGGGAAACGAATGGACTCAGCAATGCGCC507920.24010973797868843No Hit
ATTAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTCAGTAACGGCG501810.2372213490610443No Hit
TTGGCATTTGCACTTGCCTGAAAAGACAATGGTGCGAAGCTACCATCTGT426290.20152067294639922No Hit
TCTCAAAGATTAAGCCATGCATGTCTAAGTATAAGCACTAGTACTGTGAA423760.20032466247804576No Hit
AGAATGAAAGTGAAGACGGCTTCGGCCGCTGAGGCGAGATTCCGACTCTC396630.18749945931344933No Hit
ATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTATAAGCACTAGTA391160.18491361849847174No Hit
CTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTATAAGCACTAGTACTG387080.1829848743439729No Hit
AATGCGGGTTCTGCCCGGTGTCTTTGGTGATTCATAGTAACTGATCGAAT379930.17960484475949576No Hit
CCGCGGTAATTCCAGCTCCAATAGCGTATATTAAAGTTGTTGCAGTTAAA347720.16437816602998412No Hit
AGTTAAAAAGTACGTGAAACCGTTTGAAGGGAAACGAATGGACTCAGCAA346180.16365015965794288No Hit
GATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGAAGGCAG342700.1620050543496939No Hit
CTTGAGATTCAGCCGGGCGGTTGTGGCGGTCCGGGGCTTGCGGATCTGAA340580.16100286375961118No Hit
CTGATGAAGTGTTGTTGCAATAGTAATTCTGCTCAGTACGAGAGGAACCG333550.15767956194438398No Hit
TTAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTCAGTAACGGCGA333270.1575471971494674No Hit
TAACGGCGAGTGAAGCGGGAATAGCTCAAATTTGAAATCTCCGACGCTTG332780.15731555875836337No Hit
GCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTATAAGCACTAGTACT329310.15567518076421855No Hit
CCAAAGTCGACGCTCATCAGACCCCACAAAAGGTGTTGGTTGCTATAGAC328440.1552639044371563No Hit
GAGTTAAAAAGTACGTGAAACCGTTTGAAGGGAAACGAATGGACTCAGCA326880.15452644343690675No Hit
CGCAATGTGATTTCTGCCCAGTGCTCTGAATGTCAAAGTGAAGAAATTCA322020.15222896878228312No Hit
GATTGACAGATTGAGAGCTCTTTCTTGATTCTATGGGTGGTGGTGCATGG321270.15187442022447084No Hit
GCCCAGTGCTCTGAATGTCAAAGTGAAGAAATTCAACCAAGCGCGGGTAA318080.1503664070252426No Hit
TGAGATTCAGCCGGGCGGTTGTGGCGGTCCGGGGCTTGCGGATCTGAATG315360.14908057758891002No Hit
AAGCCATGCATGTCTAAGTATAAGCACTAGTACTGTGAAACTGCGAATGG307310.14527508973505815No Hit
TGTCTCAAAGATTAAGCCATGCATGTCTAAGTATAAGCACTAGTACTGTG298520.14111978063749817No Hit
CTAGTAGCTGGTTCCCTCCGAAGTTTCCCTCAGGATAGCTGGAACTCAGT287510.1359150078088138No Hit
AAAATGGTGGTAAACTCCATCTAAAGCTAAATACTGACGTGAGACCGATA285850.1351302736675226No Hit
AGTGAGGATTGACAGATTGAGAGCTCTTTCTTGATTCTATGGGTGGTGGT283450.1339957182825233No Hit
TAAAAAGTACGTGAAACCGTTTGAAGGGAAACGAATGGACTCAGCAATGC282820.13369789749396097No Hit
TACTTATTGGATGAAGTGGAAGCGGACCGCAAGGTCCACGTTCTGGACTT271240.12822366776133928No Hit
GACAGGTTAGTTTTACCCTACTGATGAAGTGTTGTTGCAATAGTAATTCT269820.12755238915854802No Hit
ATAGGGTGAAGCCAGAGGAAACTCTGGTGGAGGCTCGTAGCGATTCTGAC269750.12751929795981887No Hit
TGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTATAAGCACTAGTAC269210.12726402299819403No Hit
TGGAGTGATTTGTCTGGTTAATTCCGTTAACGAACGAGACCTTAACCTGC267040.1262381958375905No Hit
GATTCCCTCAGTAACGGCGAGTGAAGCGGGAATAGCTCAAATTTGAAATC262620.12414872300355008No Hit
AGGCGAGATTCCGACTCTCGTAGCCGGACGCATCGTTGACCGACCTATTC261810.12376581056111281No Hit
GAATAGCTCAAATTTGAAATCTCCGACGCTTGCGTCGGCGAATTGTAATT257500.12172833818221819No Hit
GATTTGTCTGGTTAATTCCGTTAACGAACGAGACCTTAACCTGCTAAATA257010.12149669979111417No Hit
AGTGCCAAAGTCGACGCTCATCAGACCCCACAAAAGGTGTTGGTTGCTAT256600.12130287991284346No Hit
TGAATTTAAGCATATTAATAAGCGGAGGAAAAGAAACTAACAAGGATTCC253350.11976650282899022No Hit
GAGGATCCATTGGAGGGCAAGTCTGGTGCCAGCAGCCGCGGTAATTCCAG253230.11970977505974024No Hit
TTGACAGATTGAGAGCTCTTTCTTGATTCTATGGGTGGTGGTGCATGGCC249270.11783775867449137No Hit
AATTTAAGCATATTAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCT249150.11778103090524142No Hit
CAGTTTTATCAGGTAAAGCGAATGATTAGAGGCATTAGGGTTAAAACAAC248660.11754939251413737No Hit
CTTGAAACCCAGAGGCAGAATGAAAGTGAAGACGGCTTCGGCCGCTGAGG247630.11706247916140851No Hit
TGGGTCTCTCGGGCTGAGACTTGAAGCGGGTGGAGCCGGCCTGGGCTGGC243970.11533228219928456No Hit
GAACGAGACCTTAACCTGCTAAATAGTTACGCGAATCTCGATTCGTGGTA239760.11334208296143158No Hit
GTCTAAGTATAAGCACTAGTACTGTGAAACTGCGAATGGCTCATTAAATC234760.11097842590934968No Hit
AAAAAGTACGTGAAACCGTTTGAAGGGAAACGAATGGACTCAGCAATGCG233980.1106096954092249No Hit
GCCGCTGAGGCGAGATTCCGACTCTCGTAGCCGGACGCATCGTTGACCGA230960.10918204654976743No Hit
TTTGAAATCTCCGACGCTTGCGTCGGCGAATTGTAATTGCGAGAAGCACT228840.10817985595968471No Hit
AAGCACTAGTACTGTGAAACTGCGAATGGCTCATTAAATCAGTTATCGTT224760.10625111180518589No Hit
ACCTATTCTCAAACTTTAAATTGGTAAGATGTCCGACTTGCTTAATTGAA224480.10611874701026927No Hit
GGATTGACAGATTGAGAGCTCTTTCTTGATTCTATGGGTGGTGGTGCATG220900.10442636856097863No Hit
GTAACTTCGGGAAAAGGATTGGCTCTAAGCGTTGGGTCTCTCGGGCTGAG219020.10353763350939583No Hit
AGGAGGGCGCAGTGGTCGTGACGCAGCCTTTGGCGCGAGCCTGGGTGAAA215920.10207216613710507No Hit
GGGATAACTGGCTTGTGGCAGCCAAGCGTTCATAGCGACGTTGCTTTTTG213500.10092815612389743No Hit
GCGAATGATTAGAGGCATTAGGGTTAAAACAACCTTAACCTATTCTCAAA211630.10004414838641879No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)