FastQCFastQC Report
Mon 24 Feb 2025
POR-262-TP3_R1_001.fastp-trim.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamePOR-262-TP3_R1_001.fastp-trim.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21153661
Total Bases2.8 Gbp
Sequences flagged as poor quality0
Sequence length15-135
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACATGGTACGAGATCACCACCCCAACGACCGGCAGCAGTCGCAGAGCAC1827120.8637370145999788No Hit
CTCCAGCAACATTAGAGTTTGGTATTGGCAGTGCCTTGGGAGTTTTTGTC1304180.6165268508368362No Hit
GAGGCCGTTTCACCCAGGCTCGCGCCAAAGGCTGCGTCACGACCACTGCG881050.4165000091473528No Hit
TCTAAGTTCGTTGTTCGTTGCCTCCCGATTTGACCTCGCGGCCATAGCTG861310.4071682911057334No Hit
TTTGGTTTCCCATAAGGTGCCAAAGGGGTCATCCAATAACACCCTCTGAT801750.37901240830133376No Hit
TTTATCTCCAGCAACATTAGAGTTTGGTATTGGCAGTGCCTTGGGAGTTT760500.359512237621658No Hit
AAGTTCGTTGTTCGTTGCCTCCCGATTTGACCTCGCGGCCATAGCTGGGC743960.3516932600933711No Hit
ATGGTACGAGATCACCACCCCAACGACCGGCAGCAGTCGCAGAGCACTGC628680.2971967831005706No Hit
AATGATCAAGTTTGACCAACTTCCCGGCATCCGCCGCTCTGTTGCCAGAG601230.284220305884641No Hit
CAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCACAAGCCAGTTATCCC589590.2787177122673943No Hit
GCACATGGTACGAGATCACCACCCCAACGACCGGCAGCAGTCGCAGAGCA538100.2543767719450548No Hit
TCTAAATGATCAAGTTTGACCAACTTCCCGGCATCCGCCGCTCTGTTGCC520160.24589597044218492No Hit
TAATCATTACTAAGGTTCCAGAAACCAACAAAATAGAACCAAAGTCCTAT507490.23990646347220937No Hit
AGTTCTAAGTTCGTTGTTCGTTGCCTCCCGATTTGACCTCGCGGCCATAG506200.23929663995277223No Hit
TTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAATGG504430.23845990535633524No Hit
GTTGTTCGTTGCCTCCCGATTTGACCTCGCGGCCATAGCTGGGCCAATCC486500.22998383116756954No Hit
GATTACCCACACCTCTCGGTGAAGGAGACACTCTCTGACATCGTCAGTGT485450.22948746318663232No Hit
CTAATTTTTTCAAAGTAAACGTCACAGATCCTGAGCACACTCAGTTAAGA476420.22521869855057242No Hit
CTCTAAATGATCAAGTTTGACCAACTTCCCGGCATCCGCCGCTCTGTTGC459160.21705935440678564No Hit
AGATTTCTGTTCTCATTGAGCTCACCTTAGGACACCTGCGTTATCATTTG425200.20100539570904538No Hit
CTAAATGATCAAGTTTGACCAACTTCCCGGCATCCGCCGCTCTGTTGCCA418010.19760645686815156No Hit
TCGGTTGCGTTGCCGCACTTGCCACCCACGAGGAGTGGCAATATCCGTGT416250.19677444958581874No Hit
TCACTGCCAGTTCTAAGTTCGTTGTTCGTTGCCTCCCGATTTGACCTCGC414920.19614571680996495No Hit
ACTTCCCCCGGAACCCAAAAACTTTGGTTTCCCATAAGGTGCCAAAGGGG403170.19059112273757248No Hit
GTTAAAGGATTTAAGTTGTACTCATTCCAATTACAAGACTTGTAAAAGCC387750.18330160438895188No Hit
ATCATTACTAAGGTTCCAGAAACCAACAAAATAGAACCAAAGTCCTATTC383880.18147213383064045No Hit
GCACTTTAACTTCGCGTTCGGTTCATCCCGCATCGCCAGTTCTGCTTACC380230.17974666418262067No Hit
GATTTCTGTTCTCATTGAGCTCACCTTAGGACACCTGCGTTATCATTTGA373160.17640445311097686No Hit
TAATCAGCGCGAGCTGATGACTCGCGCTTACTAGGAATTCCTCGTTTAAG367450.17370515675749934No Hit
ACTTCCTCTAAATGATCAAGTTTGACCAACTTCCCGGCATCCGCCGCTCT349640.1652858103379836No Hit
CACTCTAATTTTTTCAAAGTAAACGTCACAGATCCTGAGCACACTCAGTT342090.1617166881893399No Hit
CCGAAGACGAGAGTTAAGTCCAGAACGTGGACCTTGCGGTCCGCTTCCAC337220.1594144862206121No Hit
CTGCTGGCACCAGACTTGCCCTCCAATGGATCCTCGTTAAAGGATTTAAG337160.15938612233598715No Hit
AGCAACATTAGAGTTTGGTATTGGCAGTGCCTTGGGAGTTTTTGTCTCCC335810.15874793493192504No Hit
CCTGAGGGAAACTTCGGAGGGAACCAGCTACTAGACAGTTCGATTAGTCT329130.15559008911034358No Hit
CTACATTGTTCTATCAACCAGAGGCTGCTCACCTCGGAGACCTGCTGCGG327960.15503699336015644No Hit
CAGAGGCCGTTTCACCCAGGCTCGCGCCAAAGGCTGCGTCACGACCACTG323890.15311297651976175No Hit
CATGGTACGAGATCACCACCCCAACGACCGGCAGCAGTCGCAGAGCACTG317770.1502198602880135No Hit
AAATTGCAATGATCTATCCCCAGCACGACGATGTTTAAAAGATTACCCAC306710.14499145088880833No Hit
AAAATTGCAATGATCTATCCCCAGCACGACGATGTTTAAAAGATTACCCA305700.14451399216428776No Hit
TTAACTTCGCGTTCGGTTCATCCCGCATCGCCAGTTCTGCTTACCAAAAA304600.14399398761282975No Hit
GTCTTCTATTCTTGTTCGCCTAAGGTTATCTGGAGGTAATTATCGTTGCT302910.14319507152922606No Hit
GTTTCCTCTGGCTTCACCCTATTCAGGCATAGTTCACCATCTTTCGGGTC299750.14170124027231032No Hit
GTCCAAGAGAAGTGCGACGGTCGGAAGCGACGGGCCCGAGACCGCGGTCC296700.14025940947054036No Hit
CAACCATGGTAAGCCACTACCTTACCATCGACAGTTGATAGGGCAGAAAT294210.13908230825860357No Hit
TTGCCGCACTTGCCACCCACGAGGAGTGGCAATATCCGTGTTCCGGTTCG292820.1384252115981248No Hit
TGGCGAATTCTGCTTCGCAATGATAGGAAGAGCCGACATCGAAGGATCAA291570.1378342973351043No Hit
ACATGGTACGAGATCACCACCCCAACGACCGGCAGCAGTCGCAGAGCACT282150.13338116744898199No Hit
GCACCAGAGGCCGTTTCACCCAGGCTCGCGCCAAAGGCTGCGTCACGACC281260.13296043649371142No Hit
GATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCTAATTC264570.12507054925386202No Hit
GGTGGGTGAACAATCCATCACTTGGCGAATTCTGCTTCGCAATGATAGGA262250.12397381238169601No Hit
TACGAGATCACCACCCCAACGACCGGCAGCAGTCGCAGAGCACTGCAAGC262220.12395963043938352No Hit
CCAGCAACATTAGAGTTTGGTATTGGCAGTGCCTTGGGAGTTTTTGTCTC259100.1224847084388844No Hit
CTGCGTTATCATTTGACAGATGTGCCGCCCCAGCCAAACTCCCAACCTGA255430.12074978416265628No Hit
ATGATCAAGTTTGACCAACTTCCCGGCATCCGCCGCTCTGTTGCCAGAGT254120.12013050601501084No Hit
ACATTAGAGTTTGGTATTGGCAGTGCCTTGGGAGTTTTTGTCTCCCTTGG251740.11900540525821984No Hit
ATAGTAGATAGGGACAGTGGGAATCTCGTTAATCCATTCATGCGCGTCAC249580.11798430541172045No Hit
ACGTAATCAGCGCGAGCTGATGACTCGCGCTTACTAGGAATTCCTCGTTT247860.11717120738580428No Hit
CCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCT247660.11707666110372102No Hit
CTGCAAAGGATTTATCTCCAGCAACATTAGAGTTTGGTATTGGCAGTGCC246940.1167362944882212No Hit
GGCAGGGACGTAATCAGCGCGAGCTGATGACTCGCGCTTACTAGGAATTC242850.11480282301961822No Hit
TGACGGTGTCTTGGGCTTGGATCCTCCCGTCCGAAGACGAGAGTTAAGTC241550.11418827218607691No Hit
GAACATCTAAGGGCATCACAGACCTGTTATTGCCTTCCTGACTTTGGTTG239450.11319553622420253No Hit
AATTCTCCGTCACCCGTTGCAACCATGGTAAGCCACTACCTTACCATCGA237490.1122689826597864No Hit
TGCCCTTCCGTCAATTCCTTTAAGTTTCAGCTTTGCAACCATACTTCCCC236680.11188607021734914No Hit
AGCCTCCACCAGAGTTTCCTCTGGCTTCACCCTATTCAGGCATAGTTCAC234450.1108318791721206No Hit
GCCGCGAGAGAACCGGGCTGTAACAGTCCGAACGCGAGCGTCCGGACCTA234270.11074678751824567No Hit
CGCGAGCTGATGACTCGCGCTTACTAGGAATTCCTCGTTTAAGATCAAAA231870.10961223213324633No Hit
GCCTTCCTGACTTTGGTTGGATACCAACAGTCCCTCTAAGAAGTTTACCA228190.10787258054291407No Hit
TTTGGCACTTTAACTTCGCGTTCGGTTCATCCCGCATCGCCAGTTCTGCT227110.10736203061966437No Hit
ATACTTCCCCCGGAACCCAAAAACTTTGGTTTCCCATAAGGTGCCAAAGG223620.1057121979973112No Hit
ATGACCGCCAACGTCCTTAGCACATGGTACGAGATCACCACCCCAACGAC222510.10518746613174901No Hit
TACTTCCCCCGGAACCCAAAAACTTTGGTTTCCCATAAGGTGCCAAAGGG220760.10436018616352034No Hit
TTTCAGCTTTGCAACCATACTTCCCCCGGAACCCAAAAACTTTGGTTTCC220410.10419473016987461No Hit
TGGTACGAGATCACCACCCCAACGACCGGCAGCAGTCGCAGAGCACTGCA219140.10359436127864582No Hit
GAGATCACCACCCCAACGACCGGCAGCAGTCGCAGAGCACTGCAAGCAGT218630.10335326825933344No Hit
CCCGAGCCGCGAGAGAACCGGGCTGTAACAGTCCGAACGCGAGCGTCCGG214360.1013347051368555No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)