FastQCFastQC Report
Mon 24 Feb 2025
POR-260-TP3_R1_001.fastp-trim.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamePOR-260-TP3_R1_001.fastp-trim.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16900517
Total Bases2.2 Gbp
Sequences flagged as poor quality0
Sequence length15-135
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACATGGTACGAGATCACCACCCCAACGACCGGCAGCAGTCGCAGAGCAC1288860.7626157235308245No Hit
CTCCAGCAACATTAGAGTTTGGTATTGGCAGTGCCTTGGGAGTTTTTGTC904330.5350901395501688No Hit
TTTATCTCCAGCAACATTAGAGTTTGGTATTGGCAGTGCCTTGGGAGTTT733620.4340813952614586No Hit
TCTAAGTTCGTTGTTCGTTGCCTCCCGATTTGACCTCGCGGCCATAGCTG679820.4022480495715013No Hit
GAGGCCGTTTCACCCAGGCTCGCGCCAAAGGCTGCGTCACGACCACTGCG593190.3509892626361667No Hit
GCACATGGTACGAGATCACCACCCCAACGACCGGCAGCAGTCGCAGAGCA530980.31417973781512126No Hit
TTTGGTTTCCCATAAGGTGCCAAAGGGGTCATCCAATAACACCCTCTGAT522970.30944023783414437No Hit
AGTTCTAAGTTCGTTGTTCGTTGCCTCCCGATTTGACCTCGCGGCCATAG501220.2965708090468475No Hit
TAATCATTACTAAGGTTCCAGAAACCAACAAAATAGAACCAAAGTCCTAT481640.28498536464890395No Hit
AAGTTCGTTGTTCGTTGCCTCCCGATTTGACCTCGCGGCCATAGCTGGGC451580.2671989265180468No Hit
AGATTTCTGTTCTCATTGAGCTCACCTTAGGACACCTGCGTTATCATTTG434360.2570098891057593No Hit
TCTAAATGATCAAGTTTGACCAACTTCCCGGCATCCGCCGCTCTGTTGCC431490.2553117162037114No Hit
CAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCACAAGCCAGTTATCCC420610.24887404332068658No Hit
CTCTAAATGATCAAGTTTGACCAACTTCCCGGCATCCGCCGCTCTGTTGC388040.2296024435228816No Hit
ATGGTACGAGATCACCACCCCAACGACCGGCAGCAGTCGCAGAGCACTGC363910.21532477379242304No Hit
TCACTGCCAGTTCTAAGTTCGTTGTTCGTTGCCTCCCGATTTGACCTCGC358180.21193434496707997No Hit
AATGATCAAGTTTGACCAACTTCCCGGCATCCGCCGCTCTGTTGCCAGAG349370.2067214866858807No Hit
TTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAATGG334320.19781643366294654No Hit
CACTCTAATTTTTTCAAAGTAAACGTCACAGATCCTGAGCACACTCAGTT324300.1918876209526608No Hit
CTAATTTTTTCAAAGTAAACGTCACAGATCCTGAGCACACTCAGTTAAGA320700.18975750860165994No Hit
GTTGTTCGTTGCCTCCCGATTTGACCTCGCGGCCATAGCTGGGCCAATCC314870.1863079099887891No Hit
AAAATTGCAATGATCTATCCCCAGCACGACGATGTTTAAAAGATTACCCA311760.18446772959667446No Hit
ACTTCCTCTAAATGATCAAGTTTGACCAACTTCCCGGCATCCGCCGCTCT305150.1805566066410868No Hit
GTTAAAGGATTTAAGTTGTACTCATTCCAATTACAAGACTTGTAAAAGCC300500.177805211521044No Hit
CTAAATGATCAAGTTTGACCAACTTCCCGGCATCCGCCGCTCTGTTGCCA296620.1755094237649653No Hit
ACTTCCCCCGGAACCCAAAAACTTTGGTTTCCCATAAGGTGCCAAAGGGG282800.16733215912862312No Hit
CAGAGGCCGTTTCACCCAGGCTCGCGCCAAAGGCTGCGTCACGACCACTG267850.15848627589321676No Hit
CTACATTGTTCTATCAACCAGAGGCTGCTCACCTCGGAGACCTGCTGCGG261080.1544804812775846No Hit
CATGGTACGAGATCACCACCCCAACGACCGGCAGCAGTCGCAGAGCACTG257830.15255746318293104No Hit
ACATGGTACGAGATCACCACCCCAACGACCGGCAGCAGTCGCAGAGCACT256740.151912512498878No Hit
ATCATTACTAAGGTTCCAGAAACCAACAAAATAGAACCAAAGTCCTATTC251740.1489540231224879No Hit
AAATTGCAATGATCTATCCCCAGCACGACGATGTTTAAAAGATTACCCAC246510.1458594432347839No Hit
CCCGAGCCGCGAGAGAACCGGGCTGTAACAGTCCGAACGCGAGCGTCCGG240620.14237434274939637No Hit
TTTAAAAGATTACCCACACCTCTCGGTGAAGGAGACACTCTCTGACATCG240050.1420370749604879No Hit
CTGCTGGCACCAGACTTGCCCTCCAATGGATCCTCGTTAAAGGATTTAAG235480.13933301567046738No Hit
GATTACCCACACCTCTCGGTGAAGGAGACACTCTCTGACATCGTCAGTGT234360.13867031405015598No Hit
GATTTCTGTTCTCATTGAGCTCACCTTAGGACACCTGCGTTATCATTTGA234210.1385815593688643No Hit
TCGGTTGCGTTGCCGCACTTGCCACCCACGAGGAGTGGCAATATCCGTGT230520.1363981942090884No Hit
AGCCTCCACCAGAGTTTCCTCTGGCTTCACCCTATTCAGGCATAGTTCAC227330.13451067798695154No Hit
CAACCATGGTAAGCCACTACCTTACCATCGACAGTTGATAGGGCAGAAAT226330.1339189801116735No Hit
GCACCAGAGGCCGTTTCACCCAGGCTCGCGCCAAAGGCTGCGTCACGACC224440.13280067112739805No Hit
GGCAGGGACGTAATCAGCGCGAGCTGATGACTCGCGCTTACTAGGAATTC220210.13029778911497206No Hit
CCGAAGACGAGAGTTAAGTCCAGAACGTGGACCTTGCGGTCCGCTTCCAC214430.12687777539586512No Hit
GATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCTAATTC209070.12370627478437493No Hit
TTGCCGCACTTGCCACCCACGAGGAGTGGCAATATCCGTGTTCCGGTTCG208950.12363527103934158No Hit
GTTCTAAGTTCGTTGTTCGTTGCCTCCCGATTTGACCTCGCGGCCATAGC208410.12331575418669145No Hit
ATAGTAGATAGGGACAGTGGGAATCTCGTTAATCCATTCATGCGCGTCAC205140.12138090213453233No Hit
ATGACCGCCAACGTCCTTAGCACATGGTACGAGATCACCACCCCAACGAC204460.12097854757934327No Hit
CTGCGTTATCATTTGACAGATGTGCCGCCCCAGCCAAACTCCCAACCTGA201990.11951705382740659No Hit
GTCTTCTATTCTTGTTCGCCTAAGGTTATCTGGAGGTAATTATCGTTGCT201430.1191857030172509No Hit
CCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCT196300.11615029291707467No Hit
GTCCAAGAGAAGTGCGACGGTCGGAAGCGACGGGCCCGAGACCGCGGTCC194430.11504381789030477No Hit
TTAATCATTACTAAGGTTCCAGAAACCAACAAAATAGAACCAAAGTCCTA194000.11478938780393523No Hit
GAACATCTAAGGGCATCACAGACCTGTTATTGCCTTCCTGACTTTGGTTG193660.11458821052634072No Hit
GAAGTCTTCTATTCTTGTTCGCCTAAGGTTATCTGGAGGTAATTATCGTT191860.11352315435084027No Hit
CCTGAGGGAAACTTCGGAGGGAACCAGCTACTAGACAGTTCGATTAGTCT188690.11164747208620895No Hit
GGTGGGTGAACAATCCATCACTTGGCGAATTCTGCTTCGCAATGATAGGA186890.11058241591070854No Hit
ACGTAATCAGCGCGAGCTGATGACTCGCGCTTACTAGGAATTCCTCGTTT186410.11029840093057508No Hit
CTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTCAGCTTTGCAACCATA184770.10932801641511915No Hit
GTTTCCTCTGGCTTCACCCTATTCAGGCATAGTTCACCATCTTTCGGGTC183610.10864164687979663No Hit
TACGAGATCACCACCCCAACGACCGGCAGCAGTCGCAGAGCACTGCAAGC183300.10845822053846045No Hit
GCCTGACACCCACCCCGACGGCGAAGTATAAGTGCGACGCTTGAGCGCCA181330.10729257572416276No Hit
TTACCTGATAAAACTGTACACTGAGTTCCAGCTATCCTGAGGGAAACTTC178850.10582516499347329No Hit
TTAACTTCGCGTTCGGTTCATCCCGCATCGCCAGTTCTGCTTACCAAAAA178490.1056121537583732No Hit
TACTTCCCCCGGAACCCAAAAACTTTGGTTTCCCATAAGGTGCCAAAGGG176030.10415657698518926No Hit
CCAGTTCTAAGTTCGTTGTTCGTTGCCTCCCGATTTGACCTCGCGGCCAT175730.10397906762260588No Hit
TTCTAGCACGGATTCTGACTTAGAGGCGTTCAGTCATAATCCAACAGATG175310.10373055451498911No Hit
TAATCAGCGCGAGCTGATGACTCGCGCTTACTAGGAATTCCTCGTTTAAG174760.10340512068358618No Hit
ACTTTGGCACTTTAACTTCGCGTTCGGTTCATCCCGCATCGCCAGTTCTG174690.10336370183231672No Hit
AGCAACATTAGAGTTTGGTATTGGCAGTGCCTTGGGAGTTTTTGTCTCCC174540.10327494715102502No Hit
CCAGCAACATTAGAGTTTGGTATTGGCAGTGCCTTGGGAGTTTTTGTCTC173490.1026536643819831No Hit
GCACTTTAACTTCGCGTTCGGTTCATCCCGCATCGCCAGTTCTGCTTACC172600.10212705327298567No Hit
CAGAACATCTAAGGGCATCACAGACCTGTTATTGCCTTCCTGACTTTGGT171110.10124542343882142No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)