FastQCFastQC Report
Mon 24 Feb 2025
POR-260-TP1_R2_001.fastp-trim.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamePOR-260-TP1_R2_001.fastp-trim.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17040152
Total Bases2.2 Gbp
Sequences flagged as poor quality0
Sequence length15-135
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTAGGGTGCTTGAAACCCAGAGGCAGAATGAAAGTGAAGACGGCTTC455880.2675328248245673No Hit
GAGTCTTAGGGTGCTTGAAACCCAGAGGCAGAATGAAAGTGAAGACGGCT379770.22286773028785187No Hit
GACGAACTACTGCGAAAGCATTTGCCAAGAATGTTTTCATTAATCAAGAA338340.1985545668841452No Hit
TGAGAGCTCTTTCTTGATTCTATGGGTGGTGGTGCATGGCCGTTCTTAGT326750.19175298436305027No Hit
GAACTTTGAAGACCGAAGTGGAGAAAGGTTCCATGTGAACAGCAGTTGGA320790.18825536298033024No Hit
ATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTATAAGCACTAGTA274710.1612133506790315No Hit
CTTTCTTGATTCTATGGGTGGTGGTGCATGGCCGTTCTTAGTTGGTGGAG273050.16023918096505244No Hit
AATGCGGGTTCTGCCCGGTGTCTTTGGTGATTCATAGTAACTGATCGAAT242890.1425398083303482No Hit
CAGAATGAAAGTGAAGACGGCTTCGGCCGCTGAGGCGAGATTCCGACTCT229250.13453518489741173No Hit
TGAGGATTGACAGATTGAGAGCTCTTTCTTGATTCTATGGGTGGTGGTGC222410.13052113619643768No Hit
GTCTCAAAGATTAAGCCATGCATGTCTAAGTATAAGCACTAGTACTGTGA210350.12344373453945716No Hit
GCTTGAAACCCAGAGGCAGAATGAAAGTGAAGACGGCTTCGGCCGCTGAG208180.1221702717205809No Hit
GAGTTAAAAAGTACGTGAAACCGTTTGAAGGGAAACGAATGGACTCAGCA207040.1215012636037519No Hit
ATTAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTCAGTAACGGCG191030.11210580750688139No Hit
GATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGAAGGCAG179750.105486148245626No Hit
CCGCGGTAATTCCAGCTCCAATAGCGTATATTAAAGTTGTTGCAGTTAAA176780.10374320604651885No Hit
GCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTATAAGCACTAGTACT176300.1034615184183803No Hit
CTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTATAAGCACTAGTACTG173620.10188876249460686No Hit
AGTTAAAAAGTACGTGAAACCGTTTGAAGGGAAACGAATGGACTCAGCAA172470.10121388588552496No Hit
TGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTATAAGCACTAGTAC170620.10012821481874105No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)