FastQCFastQC Report
Mon 24 Feb 2025
POR-245-TP3_R1_001.fastp-trim.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamePOR-245-TP3_R1_001.fastp-trim.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18551669
Total Bases2.4 Gbp
Sequences flagged as poor quality0
Sequence length15-135
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACATGGTACGAGATCACCACCCCAACGACCGGCAGCAGTCGCAGAGCAC2082711.1226537084075832No Hit
CTCCAGCAACATTAGAGTTTGGTATTGGCAGTGCCTTGGGAGTTTTTGTC1302160.7019098928511499No Hit
TCTAAGTTCGTTGTTCGTTGCCTCCCGATTTGACCTCGCGGCCATAGCTG1049800.5658790052797945No Hit
TTTATCTCCAGCAACATTAGAGTTTGGTATTGGCAGTGCCTTGGGAGTTT1043930.5627148694815545No Hit
GAGGCCGTTTCACCCAGGCTCGCGCCAAAGGCTGCGTCACGACCACTGCG933980.5034479647087279No Hit
GCACATGGTACGAGATCACCACCCCAACGACCGGCAGCAGTCGCAGAGCA818390.441140902201306No Hit
AGTTCTAAGTTCGTTGTTCGTTGCCTCCCGATTTGACCTCGCGGCCATAG791110.4264360257829093No Hit
AGATTTCTGTTCTCATTGAGCTCACCTTAGGACACCTGCGTTATCATTTG672190.362333976527934No Hit
AAGTTCGTTGTTCGTTGCCTCCCGATTTGACCTCGCGGCCATAGCTGGGC628040.338535578658718No Hit
CAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCACAAGCCAGTTATCCC601280.3241110004711706No Hit
ATGGTACGAGATCACCACCCCAACGACCGGCAGCAGTCGCAGAGCACTGC581290.313335689635256No Hit
TCACTGCCAGTTCTAAGTTCGTTGTTCGTTGCCTCCCGATTTGACCTCGC537900.28994695841112733No Hit
TTTGGTTTCCCATAAGGTGCCAAAGGGGTCATCCAATAACACCCTCTGAT499060.26901083670692916No Hit
TAATCATTACTAAGGTTCCAGAAACCAACAAAATAGAACCAAAGTCCTAT485010.26143739412340744No Hit
GTTGTTCGTTGCCTCCCGATTTGACCTCGCGGCCATAGCTGGGCCAATCC457470.2465923685895862No Hit
TCTAAATGATCAAGTTTGACCAACTTCCCGGCATCCGCCGCTCTGTTGCC449500.24229625916676284No Hit
ACATGGTACGAGATCACCACCCCAACGACCGGCAGCAGTCGCAGAGCACT423110.22807112395116577No Hit
CAGAGGCCGTTTCACCCAGGCTCGCGCCAAAGGCTGCGTCACGACCACTG411680.2219099532230766No Hit
CATGGTACGAGATCACCACCCCAACGACCGGCAGCAGTCGCAGAGCACTG404450.218012729744154No Hit
CTCTAAATGATCAAGTTTGACCAACTTCCCGGCATCCGCCGCTCTGTTGC399700.2154523132123584No Hit
AATGATCAAGTTTGACCAACTTCCCGGCATCCGCCGCTCTGTTGCCAGAG384430.2072212478564597No Hit
GATTTCTGTTCTCATTGAGCTCACCTTAGGACACCTGCGTTATCATTTGA370810.19987959034844788No Hit
CTACATTGTTCTATCAACCAGAGGCTGCTCACCTCGGAGACCTGCTGCGG367120.19789055097953723No Hit
TTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAATGG366300.19744854223089037No Hit
GCACCAGAGGCCGTTTCACCCAGGCTCGCGCCAAAGGCTGCGTCACGACC353410.1905003803161861No Hit
ACTTCCTCTAAATGATCAAGTTTGACCAACTTCCCGGCATCCGCCGCTCT343690.1852609595395433No Hit
AAAATTGCAATGATCTATCCCCAGCACGACGATGTTTAAAAGATTACCCA336730.1815092755266386No Hit
CACTCTAATTTTTTCAAAGTAAACGTCACAGATCCTGAGCACACTCAGTT333030.17951484580713467No Hit
GTTCTAAGTTCGTTGTTCGTTGCCTCCCGATTTGACCTCGCGGCCATAGC332940.17946633265179537No Hit
AGCCTCCACCAGAGTTTCCTCTGGCTTCACCCTATTCAGGCATAGTTCAC332660.17931540283518427No Hit
CTAATTTTTTCAAAGTAAACGTCACAGATCCTGAGCACACTCAGTTAAGA326480.17598416616855334No Hit
CTAAATGATCAAGTTTGACCAACTTCCCGGCATCCGCCGCTCTGTTGCCA321440.1732674294695534No Hit
CCGAAGACGAGAGTTAAGTCCAGAACGTGGACCTTGCGGTCCGCTTCCAC317850.17133229360657526No Hit
TCGGTTGCGTTGCCGCACTTGCCACCCACGAGGAGTGGCAATATCCGTGT317600.17119753484174388No Hit
CCCGAGCCGCGAGAGAACCGGGCTGTAACAGTCCGAACGCGAGCGTCCGG311430.16787168852570622No Hit
GTTAAAGGATTTAAGTTGTACTCATTCCAATTACAAGACTTGTAAAAGCC304660.16422242117407332No Hit
ATGACCGCCAACGTCCTTAGCACATGGTACGAGATCACCACCCCAACGAC303350.16351628524635708No Hit
GCCTGACACCCACCCCGACGGCGAAGTATAAGTGCGACGCTTGAGCGCCA303010.16333301332618647No Hit
ATAGTAGATAGGGACAGTGGGAATCTCGTTAATCCATTCATGCGCGTCAC302820.16323059666491463No Hit
GTCTTCTATTCTTGTTCGCCTAAGGTTATCTGGAGGTAATTATCGTTGCT291480.1571179390921647No Hit
TTGCCGCACTTGCCACCCACGAGGAGTGGCAATATCCGTGTTCCGGTTCG291180.15695622857436708No Hit
CTGCGTTATCATTTGACAGATGTGCCGCCCCAGCCAAACTCCCAACCTGA290000.15632016720436312No Hit
GAAGTCTTCTATTCTTGTTCGCCTAAGGTTATCTGGAGGTAATTATCGTT289370.15598057511698812No Hit
TACGAGATCACCACCCCAACGACCGGCAGCAGTCGCAGAGCACTGCAAGC283280.1526978516056965No Hit
CCAGTTCTAAGTTCGTTGTTCGTTGCCTCCCGATTTGACCTCGCGGCCAT280610.15125862799729772No Hit
GGTGGGTGAACAATCCATCACTTGGCGAATTCTGCTTCGCAATGATAGGA278740.15025063243635922No Hit
CCTGAGGGAAACTTCGGAGGGAACCAGCTACTAGACAGTTCGATTAGTCT273500.14742608872549418No Hit
AGCAACATTAGAGTTTGGTATTGGCAGTGCCTTGGGAGTTTTTGTCTCCC269610.14532924234471842No Hit
TTAACTTCGCGTTCGGTTCATCCCGCATCGCCAGTTCTGCTTACCAAAAA269440.1452376063846331No Hit
ACTTCCCCCGGAACCCAAAAACTTTGGTTTCCCATAAGGTGCCAAAGGGG267140.1439978257481847No Hit
GTTTCCTCTGGCTTCACCCTATTCAGGCATAGTTCACCATCTTTCGGGTC266280.14353425559716487No Hit
ACTTTGGCACTTTAACTTCGCGTTCGGTTCATCCCGCATCGCCAGTTCTG263060.1417985627061371No Hit
TTCTAGCACGGATTCTGACTTAGAGGCGTTCAGTCATAATCCAACAGATG261470.14094149696180974No Hit
TTACCTGATAAAACTGTACACTGAGTTCCAGCTATCCTGAGGGAAACTTC260820.14059112417324826No Hit
CCAGCAACATTAGAGTTTGGTATTGGCAGTGCCTTGGGAGTTTTTGTCTC259270.1397556198312939No Hit
AAATTGCAATGATCTATCCCCAGCACGACGATGTTTAAAAGATTACCCAC256690.13836490937823437No Hit
GTCCAAGAGAAGTGCGACGGTCGGAAGCGACGGGCCCGAGACCGCGGTCC253240.13650523842356177No Hit
CATCGCCAGTTCTGCTTACCAAAAATGGCCCACTAGGCACTCTCATTCGT252660.13619259808915304No Hit
ATCATTACTAAGGTTCCAGAAACCAACAAAATAGAACCAAAGTCCTATTC251910.135788321794659No Hit
TTTAAAAGATTACCCACACCTCTCGGTGAAGGAGACACTCTCTGACATCG243580.1312981597504785No Hit
TTTGGCACTTTAACTTCGCGTTCGGTTCATCCCGCATCGCCAGTTCTGCT241770.13032250629309955No Hit
GATTACCCACACCTCTCGGTGAAGGAGACACTCTCTGACATCGTCAGTGT241160.12999369490691107No Hit
GTCGGGCCCGAGCCGCGAGAGAACCGGGCTGTAACAGTCCGAACGCGAGC238640.12863532655741108No Hit
GCACTTTAACTTCGCGTTCGGTTCATCCCGCATCGCCAGTTCTGCTTACC237810.128187927458171No Hit
TATCTCCAGCAACATTAGAGTTTGGTATTGGCAGTGCCTTGGGAGTTTTT237360.12794536168147458No Hit
GGAGTTTTTGTCTCCCTTGGCACTTTGGGACGCATGAAGACCGAAGTCTT237340.1279345809802881No Hit
CTGCTGGCACCAGACTTGCCCTCCAATGGATCCTCGTTAAAGGATTTAAG235570.12698048892528213No Hit
TCGACTTTGGCACTTTAACTTCGCGTTCGGTTCATCCCGCATCGCCAGTT233930.12609647142798852No Hit
TGACGGTGTCTTGGGCTTGGATCCTCCCGTCCGAAGACGAGAGTTAAGTC233470.12584851530069882No Hit
CAAAGTTCTCATTTGAATATTTGCTACTACCACCAAGATCTGCACCAGAG233250.12572992758764723No Hit
ATCTCCAGCAACATTAGAGTTTGGTATTGGCAGTGCCTTGGGAGTTTTTG230460.1242260197721294No Hit
GGCAGGGACGTAATCAGCGCGAGCTGATGACTCGCGCTTACTAGGAATTC227410.12258196284118696No Hit
GATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCTAATTC226480.12208066023601434No Hit
TTCTCATTTGAATATTTGCTACTACCACCAAGATCTGCACCAGAGGCCGT226410.12204292778186157No Hit
CACGTCAGTATTTAGCTTTAGATGGAGTTTACCACCATTTTTGGGCTGCA226340.12200519532770879No Hit
CAACCATGGTAAGCCACTACCTTACCATCGACAGTTGATAGGGCAGAAAT224100.12079775679481991No Hit
GCCCGAGCCGCGAGAGAACCGGGCTGTAACAGTCCGAACGCGAGCGTCCG222220.11978437088328818No Hit
AAGAGGCATGTGCTGCATCCGCCTCGGTCGGGCCCGAGCCGCGAGAGAAC220730.11898120864489335No Hit
GACGATCGATTTGCACGTCAGAATCGCTACGAGCCTCCACCAGAGTTTCC220570.11889496303540129No Hit
CGTTGCCGCACTTGCCACCCACGAGGAGTGGCAATATCCGTGTTCCGGTT219890.11852841919506002No Hit
ACCCGACTCTTCGAAAGCACATCGTGAGCGGCCGGCCGAGCCACAGACGG219540.11833975692429614No Hit
CTCACGACGGTCTAAACCCAGCTCACGTTCCCTATTGGTGGGTGAACAAT218920.1180055551875144No Hit
ATTTGACGATCGATTTGCACGTCAGAATCGCTACGAGCCTCCACCAGAGT217550.1172670771562386No Hit
CGTTGACTCGCGTCCAAGAGAAGTGCGACGGTCGGAAGCGACGGGCCCGA215120.11595722196207792No Hit
TGGTACGAGATCACCACCCCAACGACCGGCAGCAGTCGCAGAGCACTGCA213690.11518640182724262No Hit
TGGCGAATTCTGCTTCGCAATGATAGGAAGAGCCGACATCGAAGGATCAA213050.11484141938927436No Hit
TTTGTATTCATACTGAAAATCAAAATCAAGTGAGCTTTTACCCTTTTGTT212670.1146365860667307No Hit
TTGAATATTTGCTACTACCACCAAGATCTGCACCAGAGGCCGTTTCACCC211480.11399513434613348No Hit
CAACCTGACGGTGTCTTGGGCTTGGATCCTCCCGTCCGAAGACGAGAGTT211410.11395740189198071No Hit
CGTCGACTTTGGCACTTTAACTTCGCGTTCGGTTCATCCCGCATCGCCAG209010.11266371774959977No Hit
ATCTGCACCAGAGGCCGTTTCACCCAGGCTCGCGCCAAAGGCTGCGTCAC208220.11223788005273272No Hit
TCGCTATCGGTCTCACGTCAGTATTTAGCTTTAGATGGAGTTTACCACCA207890.11205999848315534No Hit
TAGATGGAGTTTACCACCATTTTTGGGCTGCATTCCCGAACAACCCGACT206610.11137003360721885No Hit
TTAATCATTACTAAGGTTCCAGAAACCAACAAAATAGAACCAAAGTCCTA205760.11091185380679225No Hit
TAGTAGATAGGGACAGTGGGAATCTCGTTAATCCATTCATGCGCGTCACT204910.11045367400636567No Hit
TTGTATTCATACTGAAAATCAAAATCAAGTGAGCTTTTACCCTTTTGTTC204630.11030274418975458No Hit
TTAGCACATGGTACGAGATCACCACCCCAACGACCGGCAGCAGTCGCAGA204440.11020032752848276No Hit
CGGGGCCTGACACCCACCCCGACGGCGAAGTATAAGTGCGACGCTTGAGC203440.10966129246915736No Hit
CCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCT202970.10940794599127442No Hit
ACCGGACACGAAAATCATTGTCTCCCGCGGATTTTCAAGGGCCGTCGAGA202410.1091060863580522No Hit
CGTCGTCCGGCTTCAATTAAGCAAGTCGGACATCTTACCAATTTAAAGTT201510.10862095480465934No Hit
GAGATCACCACCCCAACGACCGGCAGCAGTCGCAGAGCACTGCAAGCAGT201330.10852392849398079No Hit
CTATTTTGCCGACTTCCCTTACCTACATTGTTCTATCAACCAGAGGCTGC200410.10802801623940143No Hit
AACCTTTCTCCACTTCGGTCTTCAAAGTTCTCATTTGAATATTTGCTACT197120.10625459089422089No Hit
ACGTAATCAGCGCGAGCTGATGACTCGCGCTTACTAGGAATTCCTCGTTT193970.10455663045734591No Hit
TCGTCCGGCTTCAATTAAGCAAGTCGGACATCTTACCAATTTAAAGTTTG189960.1023950998694511No Hit
CTATAGCAACCAACACCTTTTGTGGGGTCTGATGAGCGTCGACTTTGGCA188670.10169974464292134No Hit
TACTCAAACCTTCCTAAGAGTAGAATAGGTCGGTCAACGATGCGTCCGGC187700.10117688063537571No Hit
GAACCAGCTACTAGACAGTTCGATTAGTCTTTCGCCCCTATACCCAAATT186040.10028208243689557No Hit
TCCCGTCCGAAGACGAGAGTTAAGTCCAGAACGTGGACCTTGCGGTCCGC185580.1000341263096059No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)