FastQCFastQC Report
Mon 24 Feb 2025
POR-245-TP2_R1_001.fastp-trim.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamePOR-245-TP2_R1_001.fastp-trim.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16574371
Total Bases2.2 Gbp
Sequences flagged as poor quality0
Sequence length15-135
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACATGGTACGAGATCACCACCCCAACGACCGGCAGCAGTCGCAGAGCAC1958811.1818306709799122No Hit
CTCCAGCAACATTAGAGTTTGGTATTGGCAGTGCCTTGGGAGTTTTTGTC1491370.8998048855066656No Hit
GAGGCCGTTTCACCCAGGCTCGCGCCAAAGGCTGCGTCACGACCACTGCG871880.5260410787232891No Hit
TCTAAGTTCGTTGTTCGTTGCCTCCCGATTTGACCTCGCGGCCATAGCTG864090.5213410511928326No Hit
TTTATCTCCAGCAACATTAGAGTTTGGTATTGGCAGTGCCTTGGGAGTTT815430.49198247100900544No Hit
AAGTTCGTTGTTCGTTGCCTCCCGATTTGACCTCGCGGCCATAGCTGGGC810770.48917090126678114No Hit
TTTGGTTTCCCATAAGGTGCCAAAGGGGTCATCCAATAACACCCTCTGAT688830.4155994818747571No Hit
ATGGTACGAGATCACCACCCCAACGACCGGCAGCAGTCGCAGAGCACTGC650860.3926906185459466No Hit
CAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCACAAGCCAGTTATCCC590580.3563212142409507No Hit
GCACATGGTACGAGATCACCACCCCAACGACCGGCAGCAGTCGCAGAGCA579820.34982926350568594No Hit
AATGATCAAGTTTGACCAACTTCCCGGCATCCGCCGCTCTGTTGCCAGAG527980.3185520584763066No Hit
AGTTCTAAGTTCGTTGTTCGTTGCCTCCCGATTTGACCTCGCGGCCATAG526930.3179185502725865No Hit
GTTGTTCGTTGCCTCCCGATTTGACCTCGCGGCCATAGCTGGGCCAATCC516420.3115774348239218No Hit
TCTAAATGATCAAGTTTGACCAACTTCCCGGCATCCGCCGCTCTGTTGCC486030.2932418973848238No Hit
TAATCATTACTAAGGTTCCAGAAACCAACAAAATAGAACCAAAGTCCTAT436280.2632256753514206No Hit
TCACTGCCAGTTCTAAGTTCGTTGTTCGTTGCCTCCCGATTTGACCTCGC434170.2619526255325164No Hit
AGATTTCTGTTCTCATTGAGCTCACCTTAGGACACCTGCGTTATCATTTG421070.25404885651467557No Hit
CTCTAAATGATCAAGTTTGACCAACTTCCCGGCATCCGCCGCTCTGTTGC415020.25039864257895517No Hit
TCGGTTGCGTTGCCGCACTTGCCACCCACGAGGAGTGGCAATATCCGTGT414810.25027194093821115No Hit
TTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAATGG403180.2432550834055784No Hit
CTAATTTTTTCAAAGTAAACGTCACAGATCCTGAGCACACTCAGTTAAGA401710.2423681719203703No Hit
GATTACCCACACCTCTCGGTGAAGGAGACACTCTCTGACATCGTCAGTGT395440.23858522293244191No Hit
CTAAATGATCAAGTTTGACCAACTTCCCGGCATCCGCCGCTCTGTTGCCA377960.22803881969336876No Hit
GATTTCTGTTCTCATTGAGCTCACCTTAGGACACCTGCGTTATCATTTGA364470.21989974762843187No Hit
GCACTTTAACTTCGCGTTCGGTTCATCCCGCATCGCCAGTTCTGCTTACC361850.2183189938248637No Hit
AGCAACATTAGAGTTTGGTATTGGCAGTGCCTTGGGAGTTTTTGTCTCCC356300.21497045046234334No Hit
ACTTCCCCCGGAACCCAAAAACTTTGGTTTCCCATAAGGTGCCAAAGGGG356210.21491614975916734No Hit
CCTGAGGGAAACTTCGGAGGGAACCAGCTACTAGACAGTTCGATTAGTCT348420.21021612222871083No Hit
GTTAAAGGATTTAAGTTGTACTCATTCCAATTACAAGACTTGTAAAAGCC347250.20951021308742274No Hit
ATCATTACTAAGGTTCCAGAAACCAACAAAATAGAACCAAAGTCCTATTC340790.2056126292816783No Hit
CTACATTGTTCTATCAACCAGAGGCTGCTCACCTCGGAGACCTGCTGCGG340700.2055583285785023No Hit
CATGGTACGAGATCACCACCCCAACGACCGGCAGCAGTCGCAGAGCACTG336840.20322943175339805No Hit
ACTTCCTCTAAATGATCAAGTTTGACCAACTTCCCGGCATCCGCCGCTCT333860.20143147513712586No Hit
CCGAAGACGAGAGTTAAGTCCAGAACGTGGACCTTGCGGTCCGCTTCCAC320010.19307520025948496No Hit
CAGAGGCCGTTTCACCCAGGCTCGCGCCAAAGGCTGCGTCACGACCACTG314800.1899317928867406No Hit
TAATCAGCGCGAGCTGATGACTCGCGCTTACTAGGAATTCCTCGTTTAAG309910.1869814546808443No Hit
GTTTCCTCTGGCTTCACCCTATTCAGGCATAGTTCACCATCTTTCGGGTC304920.18397078236030798No Hit
GTCCAAGAGAAGTGCGACGGTCGGAAGCGACGGGCCCGAGACCGCGGTCC300410.18124971379004368No Hit
ACATGGTACGAGATCACCACCCCAACGACCGGCAGCAGTCGCAGAGCACT299640.18078514110731564No Hit
GTCTTCTATTCTTGTTCGCCTAAGGTTATCTGGAGGTAATTATCGTTGCT299260.1805558714716836No Hit
TTGCCGCACTTGCCACCCACGAGGAGTGGCAATATCCGTGTTCCGGTTCG293670.17718319446330721No Hit
ACATTAGAGTTTGGTATTGGCAGTGCCTTGGGAGTTTTTGTCTCCCTTGG291430.1758317102953711No Hit
GCACCAGAGGCCGTTTCACCCAGGCTCGCGCCAAAGGCTGCGTCACGACC290800.17545160537313906No Hit
TTAACTTCGCGTTCGGTTCATCCCGCATCGCCAGTTCTGCTTACCAAAAA290710.17539730466996303No Hit
TGGCGAATTCTGCTTCGCAATGATAGGAAGAGCCGACATCGAAGGATCAA289720.17479999693502699No Hit
CTGCTGGCACCAGACTTGCCCTCCAATGGATCCTCGTTAAAGGATTTAAG289160.17446212589304294No Hit
TACGAGATCACCACCCCAACGACCGGCAGCAGTCGCAGAGCACTGCAAGC286530.17287533867801078No Hit
CACTCTAATTTTTTCAAAGTAAACGTCACAGATCCTGAGCACACTCAGTT286200.17267623609969876No Hit
CCAGCAACATTAGAGTTTGGTATTGGCAGTGCCTTGGGAGTTTTTGTCTC282900.17068521031657855No Hit
GGTGGGTGAACAATCCATCACTTGGCGAATTCTGCTTCGCAATGATAGGA276560.16686002744840212No Hit
CTGCGTTATCATTTGACAGATGTGCCGCCCCAGCCAAACTCCCAACCTGA268940.1622625679128336No Hit
AAATTGCAATGATCTATCCCCAGCACGACGATGTTTAAAAGATTACCCAC259420.156518760199105No Hit
AAAATTGCAATGATCTATCCCCAGCACGACGATGTTTAAAAGATTACCCA257570.15540257907826488No Hit
CTGCAAAGGATTTATCTCCAGCAACATTAGAGTTTGGTATTGGCAGTGCC249340.15043708144339235No Hit
CAACCATGGTAAGCCACTACCTTACCATCGACAGTTGATAGGGCAGAAAT245850.1483314208424561No Hit
AGCCTCCACCAGAGTTTCCTCTGGCTTCACCCTATTCAGGCATAGTTCAC243370.14683513479938393No Hit
TGACGGTGTCTTGGGCTTGGATCCTCCCGTCCGAAGACGAGAGTTAAGTC240220.14493461018822373No Hit
ATAGTAGATAGGGACAGTGGGAATCTCGTTAATCCATTCATGCGCGTCAC239210.14432523563035968No Hit
ATGACCGCCAACGTCCTTAGCACATGGTACGAGATCACCACCCCAACGAC236870.1429134173477835No Hit
TGGTACGAGATCACCACCCCAACGACCGGCAGCAGTCGCAGAGCACTGCA231340.13957694080819116No Hit
ATGATCAAGTTTGACCAACTTCCCGGCATCCGCCGCTCTGTTGCCAGAGT230130.13884689802104708No Hit
GATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCTAATTC228200.13768244960849493No Hit
GCCGCGAGAGAACCGGGCTGTAACAGTCCGAACGCGAGCGTCCGGACCTA224810.13563712312219872No Hit
GGCAGGGACGTAATCAGCGCGAGCTGATGACTCGCGCTTACTAGGAATTC221660.13373659851103853No Hit
GAGATCACCACCCCAACGACCGGCAGCAGTCGCAGAGCACTGCAAGCAGT219080.13217997835332634No Hit
CCAGTTCTAAGTTCGTTGTTCGTTGCCTCCCGATTTGACCTCGCGGCCAT216030.13033978785680617No Hit
ACCCGACTCTTCGAAAGCACATCGTGAGCGGCCGGCCGAGCCACAGACGG215750.13017085233581413No Hit
GAACATCTAAGGGCATCACAGACCTGTTATTGCCTTCCTGACTTTGGTTG212160.1280048576202379No Hit
CCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCT210570.12704554519746178No Hit
TTTGGCACTTTAACTTCGCGTTCGGTTCATCCCGCATCGCCAGTTCTGCT210090.12675594144718977No Hit
CCCACTTATTCTACACCTCTCATGTCTTTTCACAAGGTCAGACTAGAGTC209880.12662923980644575No Hit
TGCCCTTCCGTCAATTCCTTTAAGTTTCAGCTTTGCAACCATACTTCCCC209590.12645427087398972No Hit
CGTTGACTCGCGTCCAAGAGAAGTGCGACGGTCGGAAGCGACGGGCCCGA208010.12550099186267763No Hit
AATTCTCCGTCACCCGTTGCAACCATGGTAAGCCACTACCTTACCATCGA206870.12481318295578155No Hit
CCCGAGCCGCGAGAGAACCGGGCTGTAACAGTCCGAACGCGAGCGTCCGG204290.12325656279806939No Hit
TACTCAAACCTTCCTAAGAGTAGAATAGGTCGGTCAACGATGCGTCCGGC203640.12286439105290933No Hit
GTTCTAAGTTCGTTGTTCGTTGCCTCCCGATTTGACCTCGCGGCCATAGC201090.12132587112958917No Hit
ACGTAATCAGCGCGAGCTGATGACTCGCGCTTACTAGGAATTCCTCGTTT200870.12119313607738115No Hit
CGCGAGCTGATGACTCGCGCTTACTAGGAATTCCTCGTTTAAGATCAAAA200660.12106643443663713No Hit
GCCTGACACCCACCCCGACGGCGAAGTATAAGTGCGACGCTTGAGCGCCA200340.12087336526978912No Hit
AAGAGGCATGTGCTGCATCCGCCTCGGTCGGGCCCGAGCCGCGAGAGAAC200180.12077683068636512No Hit
TACTTCCCCCGGAACCCAAAAACTTTGGTTTCCCATAAGGTGCCAAAGGG199800.12054756105073308No Hit
TACATTGTTCTATCAACCAGAGGCTGCTCACCTCGGAGACCTGCTGCGGT199320.12025795730046106No Hit
AATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAATGGATCCTCGTTA198870.11998645378458103No Hit
TAAAACTGTACACTGAGTTCCAGCTATCCTGAGGGAAACTTCGGAGGGAA198470.119745117326021No Hit
ACCGGACACGAAAATCATTGTCTCCCGCGGATTTTCAAGGGCCGTCGAGA197840.11936501240378897No Hit
CAGCAACATTAGAGTTTGGTATTGGCAGTGCCTTGGGAGTTTTTGTCTCC197500.11915987641401293No Hit
TTTCAGCTTTGCAACCATACTTCCCCCGGAACCCAAAAACTTTGGTTTCC197040.11888233948666892No Hit
TCTATTCTTGTTCGCCTAAGGTTATCTGGAGGTAATTATCGTTGCTTTCT196500.11855653526761287No Hit
ATACTTCCCCCGGAACCCAAAAACTTTGGTTTCCCATAAGGTGCCAAAGG196410.11850223456443686No Hit
GGATTCTGACTTAGAGGCGTTCAGTCATAATCCAACAGATGGTAGCTTCG196280.11842380021540487No Hit
CAAAGTTCTCATTTGAATATTTGCTACTACCACCAAGATCTGCACCAGAG195030.11766962378240477No Hit
TAGATGGAGTTTACCACCATTTTTGGGCTGCATTCCCGAACAACCCGACT194180.11715678380796472No Hit
AGCCGCGAGAGAACCGGGCTGTAACAGTCCGAACGCGAGCGTCCGGACCT192670.1162457386769006No Hit
CTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTCAGCTTTGCAACCATA192610.11620953820811661No Hit
AACCTTTCTCCACTTCGGTCTTCAAAGTTCTCATTTGAATATTTGCTACT191120.11531055989998053No Hit
CGTCGTCCGGCTTCAATTAAGCAAGTCGGACATCTTACCAATTTAAAGTT189340.11423661265938839No Hit
TTCTAGCACGGATTCTGACTTAGAGGCGTTCAGTCATAATCCAACAGATG189000.11403147666961239No Hit
TATTCATACTGAAAATCAAAATCAAGTGAGCTTTTACCCTTTTGTTCTAC188340.11363327151298834No Hit
CATTAGAGTTTGGTATTGGCAGTGCCTTGGGAGTTTTTGTCTCCCTTGGC188020.11344020234614033No Hit
GCCTTCCTGACTTTGGTTGGATACCAACAGTCCCTCTAAGAAGTTTACCA187050.11285496143413226No Hit
CATCGCCAGTTCTGCTTACCAAAAATGGCCCACTAGGCACTCTCATTCGT185140.11170257984450813No Hit
CTCACGACGGTCTAAACCCAGCTCACGTTCCCTATTGGTGGGTGAACAAT185130.11169654643304412No Hit
TTCTCATTTGAATATTTGCTACTACCACCAAGATCTGCACCAGAGGCCGT184950.11158794502669211No Hit
GAAGTCTTCTATTCTTGTTCGCCTAAGGTTATCTGGAGGTAATTATCGTT183390.11064673283830802No Hit
CCTGATAAAACTGTACACTGAGTTCCAGCTATCCTGAGGGAAACTTCGGA182370.11003132486897996No Hit
CTATTTTGCCGACTTCCCTTACCTACATTGTTCTATCAACCAGAGGCTGC181190.10931938231622787No Hit
TTGGCACTTTAACTTCGCGTTCGGTTCATCCCGCATCGCCAGTTCTGCTT180810.10909011268059583No Hit
ATTTGACGATCGATTTGCACGTCAGAATCGCTACGAGCCTCCACCAGAGT180160.1086979409354358No Hit
ACTTTGGCACTTTAACTTCGCGTTCGGTTCATCCCGCATCGCCAGTTCTG178580.1077446619241237No Hit
GGCCGGAAGGACGGGACCCTAGCCACAAGAGGCATGTGCTGCATCCGCCT178340.1075998600489877No Hit
CTATAGCAACCAACACCTTTTGTGGGGTCTGATGAGCGTCGACTTTGGCA175990.10618200835494752No Hit
TCGTCCGGCTTCAATTAAGCAAGTCGGACATCTTACCAATTTAAAGTTTG175470.10586827095881948No Hit
CAGACTTGCCCTCCAATGGATCCTCGTTAAAGGATTTAAGTTGTACTCAT175340.1057898366097875No Hit
GTCGGGCCCGAGCCGCGAGAGAACCGGGCTGTAACAGTCCGAACGCGAGC175080.10563296791172347No Hit
TTACCTGATAAAACTGTACACTGAGTTCCAGCTATCCTGAGGGAAACTTC173770.10484259100993938No Hit
ACAAAGGGCAGGGACGTAATCAGCGCGAGCTGATGACTCGCGCTTACTAG172570.1041185816342593No Hit
GACGATCGATTTGCACGTCAGAATCGCTACGAGCCTCCACCAGAGTTTCC172040.10379881082666727No Hit
CAGAACATCTAAGGGCATCACAGACCTGTTATTGCCTTCCTGACTTTGGT171080.1032196033261232No Hit
CGTCAGTATTTAGCTTTAGATGGAGTTTACCACCATTTTTGGGCTGCATT170910.10311703533123519No Hit
ATAGAACCAAAGTCCTATTCCATTATTCCATGCTTATGTATTCAAGCGCT169800.10244732665873112No Hit
CGTACTTTTTAACTCTCTTTTCAAAGTTCTTTTCATCTTTCCCTCACGGT169230.10210342220528308No Hit
GATTCCCTTTCGATAGACGGCCCAAGATTGGGCACTTTGACACGGAGTTT168200.10148198082449102No Hit
CGTTGCCGCACTTGCCACCCACGAGGAGTGGCAATATCCGTGTTCCGGTT166160.10025116488583488No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)