FastQCFastQC Report
Mon 24 Feb 2025
POR-236-TP2_R1_001.fastp-trim.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamePOR-236-TP2_R1_001.fastp-trim.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19238643
Total Bases2.5 Gbp
Sequences flagged as poor quality0
Sequence length15-135
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACATGGTACGAGATCACCACCCCAACGACCGGCAGCAGTCGCAGAGCAC2305481.1983589487054778No Hit
CTCCAGCAACATTAGAGTTTGGTATTGGCAGTGCCTTGGGAGTTTTTGTC1604570.8340349160800998No Hit
TCTAAGTTCGTTGTTCGTTGCCTCCCGATTTGACCTCGCGGCCATAGCTG1106640.5752172853355614No Hit
GAGGCCGTTTCACCCAGGCTCGCGCCAAAGGCTGCGTCACGACCACTGCG1081750.5622797824150072No Hit
TTTATCTCCAGCAACATTAGAGTTTGGTATTGGCAGTGCCTTGGGAGTTT979120.5089340240889131No Hit
AAGTTCGTTGTTCGTTGCCTCCCGATTTGACCTCGCGGCCATAGCTGGGC898950.4672626858349625No Hit
TTTGGTTTCCCATAAGGTGCCAAAGGGGTCATCCAATAACACCCTCTGAT777070.4039110242858605No Hit
ATGGTACGAGATCACCACCCCAACGACCGGCAGCAGTCGCAGAGCACTGC769080.3997579247143367No Hit
GCACATGGTACGAGATCACCACCCCAACGACCGGCAGCAGTCGCAGAGCA698890.3632740625209377No Hit
CAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCACAAGCCAGTTATCCC684110.3555916079943892No Hit
AGTTCTAAGTTCGTTGTTCGTTGCCTCCCGATTTGACCTCGCGGCCATAG666090.3462250430032929No Hit
GTTGTTCGTTGCCTCCCGATTTGACCTCGCGGCCATAGCTGGGCCAATCC601150.3124700635070779No Hit
AGATTTCTGTTCTCATTGAGCTCACCTTAGGACACCTGCGTTATCATTTG543050.28227042832490834No Hit
AATGATCAAGTTTGACCAACTTCCCGGCATCCGCCGCTCTGTTGCCAGAG515660.26803345745331414No Hit
TCACTGCCAGTTCTAAGTTCGTTGTTCGTTGCCTCCCGATTTGACCTCGC514540.2674512958112482No Hit
TCGGTTGCGTTGCCGCACTTGCCACCCACGAGGAGTGGCAATATCCGTGT496770.25821467761525596No Hit
TAATCATTACTAAGGTTCCAGAAACCAACAAAATAGAACCAAAGTCCTAT484790.25198762719387224No Hit
TCTAAATGATCAAGTTTGACCAACTTCCCGGCATCCGCCGCTCTGTTGCC481250.25014758057519965No Hit
GATTTCTGTTCTCATTGAGCTCACCTTAGGACACCTGCGTTATCATTTGA453520.23573388206226392No Hit
GATTACCCACACCTCTCGGTGAAGGAGACACTCTCTGACATCGTCAGTGT447660.23268792918502618No Hit
GCACTTTAACTTCGCGTTCGGTTCATCCCGCATCGCCAGTTCTGCTTACC432690.22490671509419868No Hit
TTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAATGG427810.2223701536537686No Hit
CTCTAAATGATCAAGTTTGACCAACTTCCCGGCATCCGCCGCTCTGTTGC426750.22181917924252764No Hit
CTACATTGTTCTATCAACCAGAGGCTGCTCACCTCGGAGACCTGCTGCGG421100.21888238167317728No Hit
CTAATTTTTTCAAAGTAAACGTCACAGATCCTGAGCACACTCAGTTAAGA417920.2172294584394544No Hit
CAGAGGCCGTTTCACCCAGGCTCGCGCCAAAGGCTGCGTCACGACCACTG415720.2160859266425392No Hit
CATGGTACGAGATCACCACCCCAACGACCGGCAGCAGTCGCAGAGCACTG402500.2092143401174397No Hit
AGCAACATTAGAGTTTGGTATTGGCAGTGCCTTGGGAGTTTTTGTCTCCC400830.20834629552614498No Hit
CCGAAGACGAGAGTTAAGTCCAGAACGTGGACCTTGCGGTCCGCTTCCAC400000.20791487216639967No Hit
CCTGAGGGAAACTTCGGAGGGAACCAGCTACTAGACAGTTCGATTAGTCT378990.19699414350585953No Hit
CTAAATGATCAAGTTTGACCAACTTCCCGGCATCCGCCGCTCTGTTGCCA377410.19617287976080225No Hit
ACATGGTACGAGATCACCACCCCAACGACCGGCAGCAGTCGCAGAGCACT372500.1936207247049597No Hit
ACTTCCCCCGGAACCCAAAAACTTTGGTTTCCCATAAGGTGCCAAAGGGG367350.1909438207258173No Hit
ATCATTACTAAGGTTCCAGAAACCAACAAAATAGAACCAAAGTCCTATTC366020.19025250377586403No Hit
GTCTTCTATTCTTGTTCGCCTAAGGTTATCTGGAGGTAATTATCGTTGCT364760.18959757192853988No Hit
TTGCCGCACTTGCCACCCACGAGGAGTGGCAATATCCGTGTTCCGGTTCG364700.18956638469771492No Hit
GTTTCCTCTGGCTTCACCCTATTCAGGCATAGTTCACCATCTTTCGGGTC359680.1869570530520266No Hit
GTCCAAGAGAAGTGCGACGGTCGGAAGCGACGGGCCCGAGACCGCGGTCC357040.18558481489572837No Hit
GTTAAAGGATTTAAGTTGTACTCATTCCAATTACAAGACTTGTAAAAGCC354920.18448286607324643No Hit
GCACCAGAGGCCGTTTCACCCAGGCTCGCGCCAAAGGCTGCGTCACGACC353410.18369798743081828No Hit
TTAACTTCGCGTTCGGTTCATCCCGCATCGCCAGTTCTGCTTACCAAAAA345240.17945132616681955No Hit
TGGCGAATTCTGCTTCGCAATGATAGGAAGAGCCGACATCGAAGGATCAA335320.17429503733709284No Hit
CCAGCAACATTAGAGTTTGGTATTGGCAGTGCCTTGGGAGTTTTTGTCTC332080.172610926872545No Hit
GGTGGGTGAACAATCCATCACTTGGCGAATTCTGCTTCGCAATGATAGGA329500.17126987594707174No Hit
CACTCTAATTTTTTCAAAGTAAACGTCACAGATCCTGAGCACACTCAGTT323340.16806798691570918No Hit
TACGAGATCACCACCCCAACGACCGGCAGCAGTCGCAGAGCACTGCAAGC322180.16746503378642663No Hit
TAATCAGCGCGAGCTGATGACTCGCGCTTACTAGGAATTCCTCGTTTAAG320430.16655540622069862No Hit
ACTTCCTCTAAATGATCAAGTTTGACCAACTTCCCGGCATCCGCCGCTCT314590.16351984908706918No Hit
CTGCGTTATCATTTGACAGATGTGCCGCCCCAGCCAAACTCCCAACCTGA305760.15893012828399591No Hit
AGCCTCCACCAGAGTTTCCTCTGGCTTCACCCTATTCAGGCATAGTTCAC305390.158737807027242No Hit
AAAATTGCAATGATCTATCCCCAGCACGACGATGTTTAAAAGATTACCCA300180.15602971581727465No Hit
TGACGGTGTCTTGGGCTTGGATCCTCCCGTCCGAAGACGAGAGTTAAGTC299210.15552552225227112No Hit
CTGCTGGCACCAGACTTGCCCTCCAATGGATCCTCGTTAAAGGATTTAAG293820.15272386934982887No Hit
TTTGGCACTTTAACTTCGCGTTCGGTTCATCCCGCATCGCCAGTTCTGCT288000.14969870795980778No Hit
ATGACCGCCAACGTCCTTAGCACATGGTACGAGATCACCACCCCAACGAC283780.14750520605845224No Hit
CCCGAGCCGCGAGAGAACCGGGCTGTAACAGTCCGAACGCGAGCGTCCGG283430.14732328054530666No Hit
GCCGCGAGAGAACCGGGCTGTAACAGTCCGAACGCGAGCGTCCGGACCTA281890.146522808287466No Hit
AAATTGCAATGATCTATCCCCAGCACGACGATGTTTAAAAGATTACCCAC281220.1461745508765873No Hit
ATAGTAGATAGGGACAGTGGGAATCTCGTTAATCCATTCATGCGCGTCAC281190.1461589572611748No Hit
ACATTAGAGTTTGGTATTGGCAGTGCCTTGGGAGTTTTTGTCTCCCTTGG280260.14567555518338793No Hit
CTGCAAAGGATTTATCTCCAGCAACATTAGAGTTTGGTATTGGCAGTGCC278410.14471394889961833No Hit
TGGTACGAGATCACCACCCCAACGACCGGCAGCAGTCGCAGAGCACTGCA274430.14264519592156266No Hit
GAGATCACCACCCCAACGACCGGCAGCAGTCGCAGAGCACTGCAAGCAGT267450.139017081402259No Hit
GTTCTAAGTTCGTTGTTCGTTGCCTCCCGATTTGACCTCGCGGCCATAGC265690.1381022559647268No Hit
CAACCATGGTAAGCCACTACCTTACCATCGACAGTTGATAGGGCAGAAAT261270.13580479662728812No Hit
CCAGTTCTAAGTTCGTTGTTCGTTGCCTCCCGATTTGACCTCGCGGCCAT260590.13545134134460524No Hit
GATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCTAATTC257390.13378802236727402No Hit
CGTTGACTCGCGTCCAAGAGAAGTGCGACGGTCGGAAGCGACGGGCCCGA257150.1336632734439742No Hit
ACCCGACTCTTCGAAAGCACATCGTGAGCGGCCGGCCGAGCCACAGACGG256950.133559316007891No Hit
GGCAGGGACGTAATCAGCGCGAGCTGATGACTCGCGCTTACTAGGAATTC253060.13153734387607274No Hit
ACCGGACACGAAAATCATTGTCTCCCGCGGATTTTCAAGGGCCGTCGAGA252660.13132942900390634No Hit
GCCTGACACCCACCCCGACGGCGAAGTATAAGTGCGACGCTTGAGCGCCA251970.13097077584941932No Hit
TTCTAGCACGGATTCTGACTTAGAGGCGTTCAGTCATAATCCAACAGATG249920.12990521212956652No Hit
AGCCGCGAGAGAACCGGGCTGTAACAGTCCGAACGCGAGCGTCCGGACCT243990.12682287414969964No Hit
CCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCT242410.12600161040464236No Hit
GAACATCTAAGGGCATCACAGACCTGTTATTGCCTTCCTGACTTTGGTTG241440.12549741683963886No Hit
AAGAGGCATGTGCTGCATCCGCCTCGGTCGGGCCCGAGCCGCGAGAGAAC237350.12337148727173741No Hit
TACTCAAACCTTCCTAAGAGTAGAATAGGTCGGTCAACGATGCGTCCGGC237030.12320515537400428No Hit
GGATTCTGACTTAGAGGCGTTCAGTCATAATCCAACAGATGGTAGCTTCG236980.12317916601498348No Hit
TTGGCACTTTAACTTCGCGTTCGGTTCATCCCGCATCGCCAGTTCTGCTT236470.12291407455297133No Hit
CAAAGTTCTCATTTGAATATTTGCTACTACCACCAAGATCTGCACCAGAG232560.12088170667754476No Hit
GAAGTCTTCTATTCTTGTTCGCCTAAGGTTATCTGGAGGTAATTATCGTT231550.12035672162532463No Hit
TACATTGTTCTATCAACCAGAGGCTGCTCACCTCGGAGACCTGCTGCGGT230640.11988371529114605No Hit
TAAAACTGTACACTGAGTTCCAGCTATCCTGAGGGAAACTTCGGAGGGAA229530.1193067515208843No Hit
GTCGGGCCCGAGCCGCGAGAGAACCGGGCTGTAACAGTCCGAACGCGAGC229410.11924437705923437No Hit
CATCGCCAGTTCTGCTTACCAAAAATGGCCCACTAGGCACTCTCATTCGT229090.11907804516150125No Hit
ACGTAATCAGCGCGAGCTGATGACTCGCGCTTACTAGGAATTCCTCGTTT227420.11821000057020653No Hit
ATTTGACGATCGATTTGCACGTCAGAATCGCTACGAGCCTCCACCAGAGT227340.11816841759577326No Hit
ACTTTGGCACTTTAACTTCGCGTTCGGTTCATCCCGCATCGCCAGTTCTG226950.11796570059541102No Hit
TATTCATACTGAAAATCAAAATCAAGTGAGCTTTTACCCTTTTGTTCTAC225450.11718601982478702No Hit
TAGATGGAGTTTACCACCATTTTTGGGCTGCATTCCCGAACAACCCGACT221250.11500291366703982No Hit
TCTATTCTTGTTCGCCTAAGGTTATCTGGAGGTAATTATCGTTGCTTTCT219940.11432199246069487No Hit
CGTCGTCCGGCTTCAATTAAGCAAGTCGGACATCTTACCAATTTAAAGTT218170.11340196915135856No Hit
CCTGATAAAACTGTACACTGAGTTCCAGCTATCCTGAGGGAAACTTCGGA217230.1129133692017675No Hit
CTATTTTGCCGACTTCCCTTACCTACATTGTTCTATCAACCAGAGGCTGC217150.11287178622733424No Hit
CTATAGCAACCAACACCTTTTGTGGGGTCTGATGAGCGTCGACTTTGGCA217080.11283540112470511No Hit
TTACCTGATAAAACTGTACACTGAGTTCCAGCTATCCTGAGGGAAACTTC216330.1124455607393931No Hit
ATGATCAAGTTTGACCAACTTCCCGGCATCCGCCGCTCTGTTGCCAGAGT216060.11230521820068079No Hit
GACGATCGATTTGCACGTCAGAATCGCTACGAGCCTCCACCAGAGTTTCC215720.11212849055933934No Hit
GGCCGGAAGGACGGGACCCTAGCCACAAGAGGCATGTGCTGCATCCGCCT211620.10999736311963376No Hit
TCGTCCGGCTTCAATTAAGCAAGTCGGACATCTTACCAATTTAAAGTTTG211250.10980504186287983No Hit
CAGCAACATTAGAGTTTGGTATTGGCAGTGCCTTGGGAGTTTTTGTCTCC210990.10966989719597166No Hit
GCCTTCCTGACTTTGGTTGGATACCAACAGTCCCTCTAAGAAGTTTACCA210800.10957113763169263No Hit
AATTCTCCGTCACCCGTTGCAACCATGGTAAGCCACTACCTTACCATCGA210560.1094463887083928No Hit
CTCACGACGGTCTAAACCCAGCTCACGTTCCCTATTGGTGGGTGAACAAT208960.1086147292197272No Hit
TTCGGTTGCGTTGCCGCACTTGCCACCCACGAGGAGTGGCAATATCCGTG208650.10845359519379824No Hit
TGCCCTTCCGTCAATTCCTTTAAGTTTCAGCTTTGCAACCATACTTCCCC208540.10839641860395247No Hit
AACCTTTCTCCACTTCGGTCTTCAAAGTTCTCATTTGAATATTTGCTACT208020.10812612927013615No Hit
CGTTGCCGCACTTGCCACCCACGAGGAGTGGCAATATCCGTGTTCCGGTT207810.1080169739622488No Hit
TACTTCCCCCGGAACCCAAAAACTTTGGTTTCCCATAAGGTGCCAAAGGG205830.10698779534502512No Hit
CGCGAGCTGATGACTCGCGCTTACTAGGAATTCCTCGTTTAAGATCAAAA204650.10637444647213423No Hit
TTCTCATTTGAATATTTGCTACTACCACCAAGATCTGCACCAGAGGCCGT204000.10603658480486385No Hit
CATTAGAGTTTGGTATTGGCAGTGCCTTGGGAGTTTTTGTCTCCCTTGGC203760.105911835881564No Hit
CAGAACATCTAAGGGCATCACAGACCTGTTATTGCCTTCCTGACTTTGGT202580.10529848700867313No Hit
TCCCGTCCGAAGACGAGAGTTAAGTCCAGAACGTGGACCTTGCGGTCCGC202550.10528289339326063No Hit
TTTCAGCTTTGCAACCATACTTCCCCCGGAACCCAAAAACTTTGGTTTCC200500.10421732967340784No Hit
TATCTCCAGCAACATTAGAGTTTGGTATTGGCAGTGCCTTGGGAGTTTTT199850.10387946800613744No Hit
CTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTCAGCTTTGCAACCATA199770.10383788503170416No Hit
ATACTTCCCCCGGAACCCAAAAACTTTGGTTTCCCATAAGGTGCCAAAGG199060.1034688361336088No Hit
CGGGGCCTGACACCCACCCCGACGGCGAAGTATAAGTGCGACGCTTGAGC196630.10220575328519792No Hit
CCCACTTATTCTACACCTCTCATGTCTTTTCACAAGGTCAGACTAGAGTC196020.10188868310514415No Hit
CAGACTTGCCCTCCAATGGATCCTCGTTAAAGGATTTAAGTTGTACTCAT193640.10065158961575409No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)