FastQCFastQC Report
Mon 24 Feb 2025
POR-236-TP1_R2_001.fastp-trim.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamePOR-236-TP1_R2_001.fastp-trim.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20954901
Total Bases2.7 Gbp
Sequences flagged as poor quality0
Sequence length15-135
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTAGGGTGCTTGAAACCCAGAGGCAGAATGAAAGTGAAGACGGCTTC979180.46727970702414673No Hit
GAGTCTTAGGGTGCTTGAAACCCAGAGGCAGAATGAAAGTGAAGACGGCT766330.36570442399131353No Hit
GACGAACTACTGCGAAAGCATTTGCCAAGAATGTTTTCATTAATCAAGAA757960.36171013167754884No Hit
TGAGAGCTCTTTCTTGATTCTATGGGTGGTGGTGCATGGCCGTTCTTAGT697910.33305335109910567No Hit
GAACTTTGAAGACCGAAGTGGAGAAAGGTTCCATGTGAACAGCAGTTGGA697720.3329626801863679No Hit
ATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTATAAGCACTAGTA591070.2820676652206565No Hit
CTTTCTTGATTCTATGGGTGGTGGTGCATGGCCGTTCTTAGTTGGTGGAG558120.26634342009060313No Hit
AATGCGGGTTCTGCCCGGTGTCTTTGGTGATTCATAGTAACTGATCGAAT529640.2527523274865388No Hit
CAGAATGAAAGTGAAGACGGCTTCGGCCGCTGAGGCGAGATTCCGACTCT518700.247531591774163No Hit
GTCTCAAAGATTAAGCCATGCATGTCTAAGTATAAGCACTAGTACTGTGA461550.22025873565329657No Hit
TGAGGATTGACAGATTGAGAGCTCTTTCTTGATTCTATGGGTGGTGGTGC455120.21719024108011772No Hit
GCTTGAAACCCAGAGGCAGAATGAAAGTGAAGACGGCTTCGGCCGCTGAG439420.2096979603959952No Hit
CCGCGGTAATTCCAGCTCCAATAGCGTATATTAAAGTTGTTGCAGTTAAA398710.19027052430359848No Hit
GATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGAAGGCAG391400.1867820802398446No Hit
CTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTATAAGCACTAGTACTG375660.17927071094251412No Hit
GAGTTAAAAAGTACGTGAAACCGTTTGAAGGGAAACGAATGGACTCAGCA373120.17805858400380895No Hit
AGTGAGGATTGACAGATTGAGAGCTCTTTCTTGATTCTATGGGTGGTGGT368240.17572977319243838No Hit
GCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTATAAGCACTAGTACT352250.16809910006255815No Hit
TGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTATAAGCACTAGTAC342010.16321241508132153No Hit
ATTAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTCAGTAACGGCG341980.16319809862141557No Hit
AATTCCGTTAACGAACGAGACCTTAACCTGCTAAATAGTTACGCGAATCT340760.16261589591857295No Hit
TGGAGTGATTTGTCTGGTTAATTCCGTTAACGAACGAGACCTTAACCTGC328230.1566363878311809No Hit
TCTCAAAGATTAAGCCATGCATGTCTAAGTATAAGCACTAGTACTGTGAA318560.15202171558815764No Hit
AGTTAAAAAGTACGTGAAACCGTTTGAAGGGAAACGAATGGACTCAGCAA294440.14051128182376046No Hit
GTCTAAGTATAAGCACTAGTACTGTGAAACTGCGAATGGCTCATTAAATC287890.13738552141095775No Hit
TTGGCATTTGCACTTGCCTGAAAAGACAATGGTGCGAAGCTACCATCTGT281130.13415954577881328No Hit
ATAGGGTGAAGCCAGAGGAAACTCTGGTGGAGGCTCGTAGCGATTCTGAC281000.13409750778588742No Hit
AGAATGAAAGTGAAGACGGCTTCGGCCGCTGAGGCGAGATTCCGACTCTC278970.13312876066558366No Hit
CGCAATGTGATTTCTGCCCAGTGCTCTGAATGTCAAAGTGAAGAAATTCA277890.13261336810896887No Hit
TAAAAAGTACGTGAAACCGTTTGAAGGGAAACGAATGGACTCAGCAATGC267590.12769805020792033No Hit
TTGATTCTATGGGTGGTGGTGCATGGCCGTTCTTAGTTGGTGGAGTGATT265460.12668158155459672No Hit
GGGATAACTGGCTTGTGGCAGCCAAGCGTTCATAGCGACGTTGCTTTTTG263650.12581782180693674No Hit
GCCGCTGAGGCGAGATTCCGACTCTCGTAGCCGGACGCATCGTTGACCGA257160.12272069431394593No Hit
TGGGTCTCTCGGGCTGAGACTTGAAGCGGGTGGAGCCGGCCTGGGCTGGC255530.12194283332572174No Hit
TGAATTTAAGCATATTAATAAGCGGAGGAAAAGAAACTAACAAGGATTCC252490.12049209872191714No Hit
TTTACAAGTCTTGTAATTGGAATGAGTACAACTTAAATCCTTTAACGAGG250050.11932769331623186No Hit
GATTCCCTCAGTAACGGCGAGTGAAGCGGGAATAGCTCAAATTTGAAATC246630.11769561688695165No Hit
TCCGTTAACGAACGAGACCTTAACCTGCTAAATAGTTACGCGAATCTCGA244660.11675550268645984No Hit
GATTTGTCTGGTTAATTCCGTTAACGAACGAGACCTTAACCTGCTAAATA242420.11568654034681435No Hit
AGTGCCAAAGTCGACGCTCATCAGACCCCACAAAAGGTGTTGGTTGCTAT241660.11532385669586318No Hit
CTGATGAAGTGTTGTTGCAATAGTAATTCTGCTCAGTACGAGAGGAACCG241630.11530954023595721No Hit
AAAGTACGTGAAACCGTTTGAAGGGAAACGAATGGACTCAGCAATGCGCC240820.11492299581849612No Hit
TGTCTCAAAGATTAAGCCATGCATGTCTAAGTATAAGCACTAGTACTGTG239430.11425966650951966No Hit
CATAGTGAGGATTGACAGATTGAGAGCTCTTTCTTGATTCTATGGGTGGT238070.11361065366044917No Hit
TTACGAAAGACGAACTACTGCGAAAGCATTTGCCAAGAATGTTTTCATTA236840.11302367880430456No Hit
CCAAAGTCGACGCTCATCAGACCCCACAAAAGGTGTTGGTTGCTATAGAC228170.10888622189148017No Hit
TAACGGCGAGTGAAGCGGGAATAGCTCAAATTTGAAATCTCCGACGCTTG219300.10465332191261605No Hit
TTAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTCAGTAACGGCGA219020.10451970162016035No Hit
GCCCAGTGCTCTGAATGTCAAAGTGAAGAAATTCAACCAAGCGCGGGTAA218640.10433835979468478No Hit
CCATTACTACTTGGATAACCGTAGTAATTCTAGAGCTAATACATGCGAAA217700.10388977738429785No Hit
GACAGGTTAGTTTTACCCTACTGATGAAGTGTTGTTGCAATAGTAATTCT213700.10198091606350229No Hit
GTATAAGCACTAGTACTGTGAAACTGCGAATGGCTCATTAAATCAGTTAT212790.10154665011302129No Hit
GCTTGACTCTAGTCTGACCTTGTGAAAAGACATGAGAGGTGTAGAATAAG211150.10076401697149513No Hit
AAGCCATGCATGTCTAAGTATAAGCACTAGTACTGTGAAACTGCGAATGG209560.10000524459647889No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)