FastQCFastQC Report
Mon 24 Feb 2025
POR-236-TP1_R1_001.fastp-trim.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamePOR-236-TP1_R1_001.fastp-trim.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20954901
Total Bases2.7 Gbp
Sequences flagged as poor quality0
Sequence length15-135
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACATGGTACGAGATCACCACCCCAACGACCGGCAGCAGTCGCAGAGCAC1421910.6785572501631002No Hit
CTCCAGCAACATTAGAGTTTGGTATTGGCAGTGCCTTGGGAGTTTTTGTC947550.45218538612995596No Hit
TTTATCTCCAGCAACATTAGAGTTTGGTATTGGCAGTGCCTTGGGAGTTT745360.3556972185170429No Hit
TTTGGTTTCCCATAAGGTGCCAAAGGGGTCATCCAATAACACCCTCTGAT734620.3505719258707068No Hit
TCTAAGTTCGTTGTTCGTTGCCTCCCGATTTGACCTCGCGGCCATAGCTG732660.34963658382351703No Hit
TAATCATTACTAAGGTTCCAGAAACCAACAAAATAGAACCAAAGTCCTAT656550.31331572504207966No Hit
GAGGCCGTTTCACCCAGGCTCGCGCCAAAGGCTGCGTCACGACCACTGCG637670.30430589960792465No Hit
TCTAAATGATCAAGTTTGACCAACTTCCCGGCATCCGCCGCTCTGTTGCC593650.2832988807725696No Hit
GCACATGGTACGAGATCACCACCCCAACGACCGGCAGCAGTCGCAGAGCA552110.2634753559561078No Hit
AGTTCTAAGTTCGTTGTTCGTTGCCTCCCGATTTGACCTCGCGGCCATAG533630.2546564166540324No Hit
CTCTAAATGATCAAGTTTGACCAACTTCCCGGCATCCGCCGCTCTGTTGC523490.24981745320581566No Hit
AATGATCAAGTTTGACCAACTTCCCGGCATCCGCCGCTCTGTTGCCAGAG518900.24762703484020276No Hit
AGATTTCTGTTCTCATTGAGCTCACCTTAGGACACCTGCGTTATCATTTG509110.24295509675755564No Hit
AAAATTGCAATGATCTATCCCCAGCACGACGATGTTTAAAAGATTACCCA466870.22279752120995464No Hit
TTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAATGG449380.2144510250847761No Hit
CAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCACAAGCCAGTTATCCC445480.21258988529700043No Hit
CACTCTAATTTTTTCAAAGTAAACGTCACAGATCCTGAGCACACTCAGTT436220.20817087133935874No Hit
AAGTTCGTTGTTCGTTGCCTCCCGATTTGACCTCGCGGCCATAGCTGGGC433460.20685375702800984No Hit
ATGGTACGAGATCACCACCCCAACGACCGGCAGCAGTCGCAGAGCACTGC430450.20541733888411115No Hit
ACTTCCTCTAAATGATCAAGTTTGACCAACTTCCCGGCATCCGCCGCTCT424200.20243474307036813No Hit
CTAATTTTTTCAAAGTAAACGTCACAGATCCTGAGCACACTCAGTTAAGA421940.20135623642411862No Hit
CTAAATGATCAAGTTTGACCAACTTCCCGGCATCCGCCGCTCTGTTGCCA413450.1973046782707301No Hit
GTTAAAGGATTTAAGTTGTACTCATTCCAATTACAAGACTTGTAAAAGCC401060.19139198032956586No Hit
TCACTGCCAGTTCTAAGTTCGTTGTTCGTTGCCTCCCGATTTGACCTCGC359360.17149210106027227No Hit
ACTTCCCCCGGAACCCAAAAACTTTGGTTTCCCATAAGGTGCCAAAGGGG359300.17146346814046032No Hit
AAATTGCAATGATCTATCCCCAGCACGACGATGTTTAAAAGATTACCCAC357960.17082399959799383No Hit
ATCATTACTAAGGTTCCAGAAACCAACAAAATAGAACCAAAGTCCTATTC353380.1686383533856829No Hit
GATTACCCACACCTCTCGGTGAAGGAGACACTCTCTGACATCGTCAGTGT324090.15466071636415749No Hit
GTTGTTCGTTGCCTCCCGATTTGACCTCGCGGCCATAGCTGGGCCAATCC323290.15427894409999837No Hit
TTTAAAAGATTACCCACACCTCTCGGTGAAGGAGACACTCTCTGACATCG306010.14603266319416158No Hit
GGCAGGGACGTAATCAGCGCGAGCTGATGACTCGCGCTTACTAGGAATTC296070.14128914281198465No Hit
CTACATTGTTCTATCAACCAGAGGCTGCTCACCTCGGAGACCTGCTGCGG292560.13961411700298657No Hit
CATGGTACGAGATCACCACCCCAACGACCGGCAGCAGTCGCAGAGCACTG289700.13824928115861773No Hit
CTGCTGGCACCAGACTTGCCCTCCAATGGATCCTCGTTAAAGGATTTAAG286030.13649790089678782No Hit
ACATGGTACGAGATCACCACCCCAACGACCGGCAGCAGTCGCAGAGCACT285650.13631655907131224No Hit
CAGAGGCCGTTTCACCCAGGCTCGCGCCAAAGGCTGCGTCACGACCACTG285450.13622111600527245No Hit
GATTTCTGTTCTCATTGAGCTCACCTTAGGACACCTGCGTTATCATTTGA281580.13437429267740278No Hit
GATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCTAATTC281470.1343217989910809No Hit
CAACCATGGTAAGCCACTACCTTACCATCGACAGTTGATAGGGCAGAAAT278030.13268017825519673No Hit
ACGTAATCAGCGCGAGCTGATGACTCGCGCTTACTAGGAATTCCTCGTTT268420.12809413893198543No Hit
CCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCT268380.12807505031877747No Hit
TTAATCATTACTAAGGTTCCAGAAACCAACAAAATAGAACCAAAGTCCTA260530.12432890997671618No Hit
CCGAAGACGAGAGTTAAGTCCAGAACGTGGACCTTGCGGTCCGCTTCCAC254570.12148470660873081No Hit
TAATCAGCGCGAGCTGATGACTCGCGCTTACTAGGAATTCCTCGTTTAAG245800.11729952816288657No Hit
TCGGTTGCGTTGCCGCACTTGCCACCCACGAGGAGTGGCAATATCCGTGT245760.11728043954967861No Hit
CCCGAGCCGCGAGAGAACCGGGCTGTAACAGTCCGAACGCGAGCGTCCGG243560.11623056582324107No Hit
GCACCAGAGGCCGTTTCACCCAGGCTCGCGCCAAAGGCTGCGTCACGACC238870.11399242592460827No Hit
GAACATCTAAGGGCATCACAGACCTGTTATTGCCTTCCTGACTTTGGTTG237790.11347703336799349No Hit
TTGCCGCACTTGCCACCCACGAGGAGTGGCAATATCCGTGTTCCGGTTCG236230.11273257745288322No Hit
TGCCCTTCCGTCAATTCCTTTAAGTTTCAGCTTTGCAACCATACTTCCCC232820.11110527317690501No Hit
AGCCTCCACCAGAGTTTCCTCTGGCTTCACCCTATTCAGGCATAGTTCAC230560.11002676653065553No Hit
GTTCTAAGTTCGTTGTTCGTTGCCTCCCGATTTGACCTCGCGGCCATAGC229970.10974520948583819No Hit
ATAGTAGATAGGGACAGTGGGAATCTCGTTAATCCATTCATGCGCGTCAC229010.10928708276884726No Hit
CTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTCAGCTTTGCAACCATA227840.10872874083251455No Hit
GTCTTCTATTCTTGTTCGCCTAAGGTTATCTGGAGGTAATTATCGTTGCT225220.10747843666739346No Hit
TACTTCCCCCGGAACCCAAAAACTTTGGTTTCCCATAAGGTGCCAAAGGG224730.107244601155596No Hit
AAGGGCAGGGACGTAATCAGCGCGAGCTGATGACTCGCGCTTACTAGGAA222620.10623767680887637No Hit
GAAGTCTTCTATTCTTGTTCGCCTAAGGTTATCTGGAGGTAATTATCGTT218820.10442425855412059No Hit
ATACTTCCCCCGGAACCCAAAAACTTTGGTTTCCCATAAGGTGCCAAAGG214340.10228633387482956No Hit
CTGCGTTATCATTTGACAGATGTGCCGCCCCAGCCAAACTCCCAACCTGA213650.10195705529699234No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)