FastQCFastQC Report
Mon 24 Feb 2025
POR-216-TP4_R1_001.fastp-trim.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamePOR-216-TP4_R1_001.fastp-trim.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21204544
Total Bases2.8 Gbp
Sequences flagged as poor quality0
Sequence length15-135
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACATGGTACGAGATCACCACCCCAACGACCGGCAGCAGTCGCAGAGCAC1855560.8750765873578795No Hit
CTCCAGCAACATTAGAGTTTGGTATTGGCAGTGCCTTGGGAGTTTTTGTC1087950.5130739901787089No Hit
GAGGCCGTTTCACCCAGGCTCGCGCCAAAGGCTGCGTCACGACCACTGCG1013090.47777023641725097No Hit
TCTAAGTTCGTTGTTCGTTGCCTCCCGATTTGACCTCGCGGCCATAGCTG954840.45029970934531766No Hit
TTTATCTCCAGCAACATTAGAGTTTGGTATTGGCAGTGCCTTGGGAGTTT849470.40060753015957334No Hit
TTTGGTTTCCCATAAGGTGCCAAAGGGGTCATCCAATAACACCCTCTGAT847020.3994521174329427No Hit
TAATCATTACTAAGGTTCCAGAAACCAACAAAATAGAACCAAAGTCCTAT805060.3796639060005252No Hit
GCACATGGTACGAGATCACCACCCCAACGACCGGCAGCAGTCGCAGAGCA737040.34758587593300755No Hit
AGTTCTAAGTTCGTTGTTCGTTGCCTCCCGATTTGACCTCGCGGCCATAG680000.3206859812689205No Hit
AGATTTCTGTTCTCATTGAGCTCACCTTAGGACACCTGCGTTATCATTTG673850.31778565952656185No Hit
CAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCACAAGCCAGTTATCCC591100.27876100518832186No Hit
AAGTTCGTTGTTCGTTGCCTCCCGATTTGACCTCGCGGCCATAGCTGGGC559080.263660468246806No Hit
ATGGTACGAGATCACCACCCCAACGACCGGCAGCAGTCGCAGAGCACTGC554390.26144867817011297No Hit
CTAATTTTTTCAAAGTAAACGTCACAGATCCTGAGCACACTCAGTTAAGA523770.247008377072386No Hit
CACTCTAATTTTTTCAAAGTAAACGTCACAGATCCTGAGCACACTCAGTT499810.23570891220296933No Hit
TTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAATGG494770.23333206316532906No Hit
TCTAAATGATCAAGTTTGACCAACTTCCCGGCATCCGCCGCTCTGTTGCC492770.23238886910277343No Hit
GTCGGGCCCGAGCCGCGAGAGAACCGGGCTGTAACAGTCCGAACGCGAGC476680.22480087286951325No Hit
CAGAGGCCGTTTCACCCAGGCTCGCGCCAAAGGCTGCGTCACGACCACTG462910.21830698174881763No Hit
TCACTGCCAGTTCTAAGTTCGTTGTTCGTTGCCTCCCGATTTGACCTCGC449150.21181780659843474No Hit
CTCTAAATGATCAAGTTTGACCAACTTCCCGGCATCCGCCGCTCTGTTGC438100.2066066594028148No Hit
ATCATTACTAAGGTTCCAGAAACCAACAAAATAGAACCAAAGTCCTATTC435860.20555028205275247No Hit
AAAATTGCAATGATCTATCCCCAGCACGACGATGTTTAAAAGATTACCCA430470.203008374054165No Hit
AATGATCAAGTTTGACCAACTTCCCGGCATCCGCCGCTCTGTTGCCAGAG426670.20121630533530926No Hit
GTTAAAGGATTTAAGTTGTACTCATTCCAATTACAAGACTTGTAAAAGCC426320.20105124637436203No Hit
GTTGTTCGTTGCCTCCCGATTTGACCTCGCGGCCATAGCTGGGCCAATCC419470.19782080671010893No Hit
ACTTCCCCCGGAACCCAAAAACTTTGGTTTCCCATAAGGTGCCAAAGGGG413660.19508082795838477No Hit
GCACCAGAGGCCGTTTCACCCAGGCTCGCGCCAAAGGCTGCGTCACGACC387090.182550494837333No Hit
ACATGGTACGAGATCACCACCCCAACGACCGGCAGCAGTCGCAGAGCACT379720.17907482471681543No Hit
GATTTCTGTTCTCATTGAGCTCACCTTAGGACACCTGCGTTATCATTTGA378070.178296689615207No Hit
CATGGTACGAGATCACCACCCCAACGACCGGCAGCAGTCGCAGAGCACTG372780.17580194131974733No Hit
CCCGAGCCGCGAGAGAACCGGGCTGTAACAGTCCGAACGCGAGCGTCCGG368680.17386839349150823No Hit
GATTACCCACACCTCTCGGTGAAGGAGACACTCTCTGACATCGTCAGTGT365980.1725950815070581No Hit
TTTAAAAGATTACCCACACCTCTCGGTGAAGGAGACACTCTCTGACATCG360190.16986453469595952No Hit
CACGTCAGTATTTAGCTTTAGATGGAGTTTACCACCATTTTTGGGCTGCA359820.1696900437943867No Hit
AGCCTCCACCAGAGTTTCCTCTGGCTTCACCCTATTCAGGCATAGTTCAC358970.16928918631780057No Hit
CTAAATGATCAAGTTTGACCAACTTCCCGGCATCCGCCGCTCTGTTGCCA349060.16461565973783732No Hit
ACTTCCTCTAAATGATCAAGTTTGACCAACTTCCCGGCATCCGCCGCTCT347740.1639931516565506No Hit
TCGCTATCGGTCTCACGTCAGTATTTAGCTTTAGATGGAGTTTACCACCA335300.15812648458745446No Hit
GATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCTAATTC334500.1577492069624322No Hit
CCGAAGACGAGAGTTAAGTCCAGAACGTGGACCTTGCGGTCCGCTTCCAC329570.15542423359823251No Hit
TAGATGGAGTTTACCACCATTTTTGGGCTGCATTCCCGAACAACCCGACT328480.1549101928341397No Hit
TTAATCATTACTAAGGTTCCAGAAACCAACAAAATAGAACCAAAGTCCTA325460.15348596979968068No Hit
TCGGTTGCGTTGCCGCACTTGCCACCCACGAGGAGTGGCAATATCCGTGT324730.15314170396684784No Hit
CAACCATGGTAAGCCACTACCTTACCATCGACAGTTGATAGGGCAGAAAT321570.15165145734800994No Hit
AAATTGCAATGATCTATCCCCAGCACGACGATGTTTAAAAGATTACCCAC315970.14901051397285411No Hit
TTACCTGATAAAACTGTACACTGAGTTCCAGCTATCCTGAGGGAAACTTC313420.14780794154309568No Hit
CTACATTGTTCTATCAACCAGAGGCTGCTCACCTCGGAGACCTGCTGCGG312830.14752969929464174No Hit
ACCCGACTCTTCGAAAGCACATCGTGAGCGGCCGGCCGAGCCACAGACGG312570.14740708406650951No Hit
CTGCTGGCACCAGACTTGCCCTCCAATGGATCCTCGTTAAAGGATTTAAG312300.14727975286806452No Hit
TTGCCGCACTTGCCACCCACGAGGAGTGGCAATATCCGTGTTCCGGTTCG307550.14503966696949483No Hit
GTCTTCTATTCTTGTTCGCCTAAGGTTATCTGGAGGTAATTATCGTTGCT304840.14376163901473193No Hit
GTCCAAGAGAAGTGCGACGGTCGGAAGCGACGGGCCCGAGACCGCGGTCC303030.14290804838811907No Hit
TTCTAGCACGGATTCTGACTTAGAGGCGTTCAGTCATAATCCAACAGATG302570.14269111375373128No Hit
CTGCGTTATCATTTGACAGATGTGCCGCCCCAGCCAAACTCCCAACCTGA302190.1425119068818457No Hit
TTAACTTCGCGTTCGGTTCATCCCGCATCGCCAGTTCTGCTTACCAAAAA297590.14034256053796773No Hit
GTTTCCTCTGGCTTCACCCTATTCAGGCATAGTTCACCATCTTTCGGGTC295410.13931447900978206No Hit
CCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCT294160.1387249827206848No Hit
ATGACCGCCAACGTCCTTAGCACATGGTACGAGATCACCACCCCAACGAC286330.1350323779657794No Hit
GCCTGACACCCACCCCGACGGCGAAGTATAAGTGCGACGCTTGAGCGCCA285520.13465038437044438No Hit
GTTCTAAGTTCGTTGTTCGTTGCCTCCCGATTTGACCTCGCGGCCATAGC284880.13434856227042657No Hit
ATAGTAGATAGGGACAGTGGGAATCTCGTTAATCCATTCATGCGCGTCAC281770.13288189550315252No Hit
GAAGTCTTCTATTCTTGTTCGCCTAAGGTTATCTGGAGGTAATTATCGTT280330.13220279577811248No Hit
CCTGAGGGAAACTTCGGAGGGAACCAGCTACTAGACAGTTCGATTAGTCT278710.1314388085874424No Hit
CGTTGACTCGCGTCCAAGAGAAGTGCGACGGTCGGAAGCGACGGGCCCGA271130.1278641030903565No Hit
ACTTTGGCACTTTAACTTCGCGTTCGGTTCATCCCGCATCGCCAGTTCTG271020.12781222741691592No Hit
GCCCGAGCCGCGAGAGAACCGGGCTGTAACAGTCCGAACGCGAGCGTCCG268800.12676528200747914No Hit
TTTGGCACTTTAACTTCGCGTTCGGTTCATCCCGCATCGCCAGTTCTGCT267470.12613805795587965No Hit
GCACTTTAACTTCGCGTTCGGTTCATCCCGCATCGCCAGTTCTGCTTACC266730.12578907615273405No Hit
CATCGCCAGTTCTGCTTACCAAAAATGGCCCACTAGGCACTCTCATTCGT262360.12372819712604996No Hit
GGCATCGTTTATGGTTAGAACTAGGACGGTATCTGATCGTCTTCGATCCT260630.12291233426193933No Hit
GGTGGGTGAACAATCCATCACTTGGCGAATTCTGCTTCGCAATGATAGGA258640.12197385616969644No Hit
GGCAGGGACGTAATCAGCGCGAGCTGATGACTCGCGCTTACTAGGAATTC257220.12130418838528195No Hit
TGACGGTGTCTTGGGCTTGGATCCTCCCGTCCGAAGACGAGAGTTAAGTC256430.12093162673057246No Hit
TACTTCCCCCGGAACCCAAAAACTTTGGTTTCCCATAAGGTGCCAAAGGG255770.1206203726899291No Hit
ATACTTCCCCCGGAACCCAAAAACTTTGGTTTCCCATAAGGTGCCAAAGG254000.11978564594456735No Hit
TACGAGATCACCACCCCAACGACCGGCAGCAGTCGCAGAGCACTGCAAGC252300.11898393099139505No Hit
CAAAGTTCTCATTTGAATATTTGCTACTACCACCAAGATCTGCACCAGAG252200.11893677128826728No Hit
CATCGTTTATGGTTAGAACTAGGACGGTATCTGATCGTCTTCGATCCTCT249780.11779550647257493No Hit
CTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTCAGCTTTGCAACCATA244640.11537149773180692No Hit
GACGATCGATTTGCACGTCAGAATCGCTACGAGCCTCCACCAGAGTTTCC243410.1147914333833352No Hit
TCGACTTTGGCACTTTAACTTCGCGTTCGGTTCATCCCGCATCGCCAGTT241850.1140557420145418No Hit
ATTTGACGATCGATTTGCACGTCAGAATCGCTACGAGCCTCCACCAGAGT241850.1140557420145418No Hit
CGTTGCCGCACTTGCCACCCACGAGGAGTGGCAATATCCGTGTTCCGGTT241100.11370204424108342No Hit
TGCCCTTCCGTCAATTCCTTTAAGTTTCAGCTTTGCAACCATACTTCCCC239160.11278714600040443No Hit
CCAGTTCTAAGTTCGTTGTTCGTTGCCTCCCGATTTGACCTCGCGGCCAT238690.11256549539570386No Hit
CGTACTTTTTAACTCTCTTTTCAAAGTTCTTTTCATCTTTCCCTCACGGT236900.11172133670971655No Hit
AGCAACATTAGAGTTTGGTATTGGCAGTGCCTTGGGAGTTTTTGTCTCCC236750.1116505971550249No Hit
TACTCAAACCTTCCTAAGAGTAGAATAGGTCGGTCAACGATGCGTCCGGC236010.11130161535187931No Hit
ACGTAATCAGCGCGAGCTGATGACTCGCGCTTACTAGGAATTCCTCGTTT235220.11092905369716982No Hit
GAACATCTAAGGGCATCACAGACCTGTTATTGCCTTCCTGACTTTGGTTG233520.11012733874399751No Hit
ATAGAACCAAAGTCCTATTCCATTATTCCATGCTTATGTATTCAAGCGCT231930.10937749946426578No Hit
TTCTCATTTGAATATTTGCTACTACCACCAAGATCTGCACCAGAGGCCGT231460.10915584885956518No Hit
CGTCAGTATTTAGCTTTAGATGGAGTTTACCACCATTTTTGGGCTGCATT230530.1087172636204768No Hit
AGCTTTAGATGGAGTTTACCACCATTTTTGGGCTGCATTCCCGAACAACC228970.10798157225168342No Hit
ATCTGCACCAGAGGCCGTTTCACCCAGGCTCGCGCCAAAGGCTGCGTCAC225630.10640643816721548No Hit
CCAGCAACATTAGAGTTTGGTATTGGCAGTGCCTTGGGAGTTTTTGTCTC224300.10577921411561597No Hit
TTGAATATTTGCTACTACCACCAAGATCTGCACCAGAGGCCGTTTCACCC223570.10543494828278316No Hit
CAACCTGACGGTGTCTTGGGCTTGGATCCTCCCGTCCGAAGACGAGAGTT222930.10513312618276535No Hit
CTATCGGTCTCACGTCAGTATTTAGCTTTAGATGGAGTTTACCACCATTT222270.10482187214212199No Hit
GCACATCGTGAGCGGCCGGCCGAGCCACAGACGGGGTTGTCACCCTCTAT222080.1047322687061792No Hit
CTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCTA221810.10460493750773418No Hit
AATTCTCCGTCACCCGTTGCAACCATGGTAAGCCACTACCTTACCATCGA216860.10227053220290896No Hit
CGAGCCGCGAGAGAACCGGGCTGTAACAGTCCGAACGCGAGCGTCCGGAC215260.10151597695286445No Hit
TGGCGAATTCTGCTTCGCAATGATAGGAAGAGCCGACATCGAAGGATCAA214490.10115284723878051No Hit
TTTGTATTCATACTGAAAATCAAAATCAAGTGAGCTTTTACCCTTTTGTT213160.10052562318718102No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)