FastQCFastQC Report
Wed 30 Apr 2025
2H2_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename2H2_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20324284
Total Bases3 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC69

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAAAATCTTTGCTCTGAAGTGGAAAGATCGGAAGAGCACACGTCTGAAC276148913.587140388315772Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTGCTACGATCTTCTGAGATTAAGCCTTTGTTCTAAGATTTGTAGATCGG4897942.4098954728245285No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCATCTGGGGGG4704982.3149548589263955TruSeq Adapter, Index 16 (97% over 43bp)
GCACTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT3755411.8477452883457053No Hit
TTGTTTAAATATTCTGTTGATGAACTCAGCAGATCGGAAGAGCACACGTC2815591.3853329347297056No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCATCTAGGGGG2767141.3614944565820868TruSeq Adapter, Index 16 (97% over 43bp)
GTTGCTACGATCTTCTGAGATTAAGCCTTTGTTCTAAGATTTGTAGATCG2676221.3167597933585262No Hit
AACTTTTGACGGTGGATCTCTTGGCTCACGTATCGATGAAGAACGCAGCC1277220.6284206617069511No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTC881730.43383078095149624No Hit
GCTACGATCTTCTGAGATTAAGCCTTTGTTCTAAGATTTGTAGATCGGAA791060.38921912329113295No Hit
GGTGACCGCCTGGGAATACCCCGTGTTGTAGGCTTAGATCGGAAGAGCAC728040.35821188092038075No Hit
TGAAAATCTTTGCTCTGAAGTGGAAGATCGGAAGAGCACACGTCTGAACT710850.34975401839494075Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCAGGTCTAGGCTGGTTAGTTTAGATCGGAAGAGCACACGTCTGAACTCC680360.33475225990740926Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TATTAATGCTGTAGGTGAAAGACCAAAAAAAAGATCGGAAGAGCACACGT642690.31621778164485403No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGT621710.3058951547813443No Hit
GAGGTCCGGACGGTCGAGGGTTATCATCCTTAGATCGGAAGAGCACACGT602280.29633516240965735No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACG598030.29424406783530477No Hit
TGCTACGATCTTCTGAGATTAAGCCTTTGTTCTAAGATTTGTAGATCGGA486520.2393786664268222No Hit
CTTCGGTACGCCCCTCCCCGCCTTCATTGACCTCATTAGATCGGAAGAGC467380.22996136050844398No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCATCTGGGGGGGGGGG444940.2189203811558626TruSeq Adapter, Index 16 (97% over 39bp)
TAAAATGTAAAATATGACGCTCCTAAAAGGAGATCGGAAGAGCACACGTC414590.2039875057837216No Hit
GAGGTCCGGACTTGGGGAGGGTTATCTACTAGATCGGAAGAGCACACGTC410570.2020095763275105No Hit
TGAAAATCTTTGCTCTGAAGTGGGAAGATCGGAAGAGCACACGTCTGAAC362990.17859915753981787Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTGTTTAAATATTCTGTTGATGAACTCAGTAGATCGGAAGAGCACACGTC347070.17076616327541969No Hit
GTATAATTGTGAGGTGTGTCTTTTCTTAAAGCAGATCGGAAGAGCACACG323320.15908063477168494No Hit
TGAAAATCTTTGCTCTGAAGTGGAAAGATCGGAGAGCACACGTCTGAACT318280.15660084261762924No Hit
TTGCTACGATCTTCTGAGATTAAGCCTTTGTTCTAAGATTTGCAGATCGG313900.15444578515041416No Hit
TGTTGCTACGATCTTCTGAGATTAAGCCTTTGTTCTAAGATTTGTAGATC308800.15193647166119112No Hit
AGCACTGATGACTGTTCAGTTTTTCTGAATTAGATCGGAAGAGCACACGT286970.14119562588281093No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTC280400.1379630396819883No Hit
TCCCGTAGATCCGAACTTGTAGATCGGAAGAGCACACGTCTGAACTCCAG279300.13742181520392058Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GAGGTCCGGACGGAGGAGGGTTATCCACAGATCGGAAGAGCACACGTCTG277390.13648205270109393Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCATCTGGTGGG264310.13004640163461603TruSeq Adapter, Index 16 (97% over 43bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCATCTGGGGTG262690.1292493255850981TruSeq Adapter, Index 16 (97% over 43bp)
TATTAATGCTGTAGGTGAAAGACAAGATCGGAAGAGCACACGTCTGAACT251550.12376819768903052Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TGAAAATCTTTGCTCTGAAGTGGAAAGATCGGAAGAGCACACGTCTAAAC247920.12198215691140706Illumina Multiplexing PCR Primer 2.01 (96% over 25bp)
TGACTGTTAGTGAGGAATGTGAAATTGTCCTCTGATGATAAGATCGGAAG245910.12099319218330151No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCAGATCGGAAGAGCACACGTCTG225650.1110248213417998Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCATCTAGTGGG220700.10858931119049507TruSeq Adapter, Index 16 (97% over 43bp)
GCCTACGACCATACCACAGGGAGAACACCGGTTCTAGATCGGAAGAGCAC214830.10570114056662464No Hit
TGACTGTTAGTGAGGAATGTGAAATTGTCCTCTGATGATAGATCGGAAGA212340.10447600515718043No Hit
TGAAAATCTTTGCTCTGAAGTGGAAAGATCGGAAAGCACACGTCTGAACT210550.10359528532468842No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)