FastQCFastQC Report
Wed 30 Apr 2025
2H1_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename2H1_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15270
Total Bases2.2 Mbp
Sequences flagged as poor quality0
Sequence length150
%GC70

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA496332.501637197118534Illumina RNA PCR Primer (100% over 45bp)
TCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATCATT4613.0189914865749836Illumina RNA PCR Primer (100% over 50bp)
TTCCACTTCAGAGCAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA3342.1872953503601833Illumina DpnII expression Sequencing Primer (100% over 25bp)
GGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAAC1140.7465618860510805No Hit
GGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGT1080.7072691552062869Illumina RNA PCR Primer (100% over 44bp)
GAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAACTCT960.6286836935166994Illumina DpnII expression Sequencing Primer (100% over 22bp)
AACCATACAACCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT850.5566470203012442Illumina DpnII expression Sequencing Primer (96% over 28bp)
AACTATACAATCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT790.5173542894564506Illumina DpnII expression Sequencing Primer (96% over 28bp)
AACAGCACAAACTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT750.4911591355599214Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGAATCATACCACTAGACCAAACAGCCGATCGTCGGACTGTAGAACTCTG470.30779305828421744Illumina DpnII expression Sequencing Primer (100% over 23bp)
ACAAAGTTCTGTAGTGCACTGAGATCGTCGGACTGTAGAACTCTGAACGT460.3012442698100851Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGCCTATCCTGGATTACTTGAAGATCGTCGGACTGTAGAACTCTGAACGT420.275049115913556Illumina DpnII expression Sequencing Primer (96% over 28bp)
GGCGAGAATTCTACCACTGAACCACAGTGCGATCGTCGGACTGTAGAACT410.2685003274394237No Hit
AAGCCTACGACACGGGGTATTCCCAGGCGGTCACCCAGATCGTCGGACTG390.2554027504911591No Hit
GAGAATTCTACCACTGAACCACAGTGCGATCGTCGGACTGTAGAACTCTG360.2357563850687623Illumina DpnII expression Sequencing Primer (100% over 23bp)
GATCAGCAAGAGTATTCTCTTCATTTCAGGTCAGTCCCGAAAGATGATTT360.2357563850687623No Hit
GAGGTCAAGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGG340.2226588081204977Illumina RNA PCR Primer (100% over 37bp)
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCAGTA310.20301244269810087Illumina RNA PCR Primer (97% over 45bp)
GCAGAACTTAGCCACTGTGAAGATCGTCGGACTGTAGAACTCTGAACGTG300.19646365422396855Illumina DpnII expression Sequencing Primer (96% over 29bp)
AACTATACAACCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGT280.183366077275704Illumina DpnII expression Sequencing Primer (96% over 28bp)
CATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA260.17026850032743943Illumina RNA PCR Primer (100% over 45bp)
ACAAATTCGGATCTACAGGGTAGATCGTCGGACTGTAGAACTCTGAACGT240.15717092337917485Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAAC240.15717092337917485No Hit
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGTCGTA240.15717092337917485Illumina RNA PCR Primer (97% over 45bp)
ACTATACAATCTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGTG230.15062213490504256Illumina DpnII expression Sequencing Primer (96% over 29bp)
AGCAAAAATGTGCTAGTGCCAAAGATCGTCGGACTGTAGAACTCTGAACG230.15062213490504256Illumina Small RNA Adapter 1 (100% over 26bp)
TTCCGCCTCAGAAGAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA220.1440733464309103Illumina DpnII expression Sequencing Primer (100% over 25bp)
ACGGCCTGATTCACAACACCAGCTGATCGTCGGACTGTAGAACTCTGAAC220.1440733464309103Illumina DpnII expression Sequencing Primer (100% over 26bp)
TATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA190.12442698100851343Illumina RNA PCR Primer (100% over 45bp)
CTTCCAGTCGAGGATGTTTACAGATCGTCGGACTGTAGAACTCTGAACGT190.12442698100851343Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGAGCTACAGTGCTTCATCTCAGATCGTCGGACTGTAGAACTCTGAACGT180.11787819253438114Illumina DpnII expression Sequencing Primer (96% over 28bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG170.11132940406024885No Hit
ACAGCACAAACTACTACCTCAGATCGTCGGACTGTAGAACTCTGAACGTG170.11132940406024885Illumina DpnII expression Sequencing Primer (96% over 29bp)
TGGATCGTAGAACCAAGGAAGAATCTCAGCCGATCGTAACGACAAGGATC160.10478061558611657No Hit
CATACAGCTAGATAACCAAAGAGATCGTCGGACTGTAGAACTCTGAACGT160.10478061558611657Illumina DpnII expression Sequencing Primer (96% over 28bp)
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTTGCCGTA160.10478061558611657Illumina RNA PCR Primer (97% over 45bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)