FastQCFastQC Report
Wed 30 Apr 2025
2H1_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename2H1_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15270
Total Bases2.2 Mbp
Sequences flagged as poor quality0
Sequence length150
%GC71

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTGGGGGG424527.79960707269155TruSeq Adapter, Index 8 (97% over 45bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTAGGGGG5073.320235756385069TruSeq Adapter, Index 8 (97% over 45bp)
TGAAAATCTTTGCTCTGAAGTGGAAAGATCGGAAGAGCACACGTCTGAAC3312.1676489849377867Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTGGGGGGGGGGG2491.630648330058939TruSeq Adapter, Index 8 (97% over 41bp)
GCACTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT1180.7727570399476097No Hit
CAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTGGGGG990.6483300589390962TruSeq Adapter, Index 8 (97% over 45bp)
TGAGGTAGTAGGTTGTATGGTTAGATCGGAAGAGCACACGTCTGAACTCC900.5893909626719057Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCACTGGTGGTTCAGTGGTAGAATTCTCAGATCGGAAGAGCACACGTCTG900.5893909626719057Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGAGGTAGTAGATTGTATAGTTAGATCGGAAGAGCACACGTCTGAACTCC850.5566470203012442Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTGGGGTG800.5239030779305829TruSeq Adapter, Index 8 (97% over 45bp)
TGAGGTAGTAGTTTGTGCTGTTAGATCGGAAGAGCACACGTCTGAACTCC730.4780615586116569Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCAGTGCACTACAGAACTTTGTAGATCGGAAGAGCACACGTCTGAACTCC480.3143418467583497Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGCTGTTTGGTCTAGTGGTATGATTCTAGATCGGAAGAGCACACGTCTGA470.30779305828421744Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTCAAGTAATCCAGGATAGGCTAGATCGGAAGAGCACACGTCTGAACTCC420.275049115913556Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCACTGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTC420.275049115913556No Hit
TGGGTGACCGCCTGGGAATACCCCGTGTCGTAGGCTTAGATCGGAAGAGC410.2685003274394237No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCAGATCGGAAGAGCACACGTCTGA370.24230517354289455Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCGGGGGGG350.22920759659463003TruSeq Adapter, Index 8 (97% over 42bp)
TTGACCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAAT330.21611001964636545TruSeq Adapter, Index 8 (100% over 41bp)
TGAGGTAGTAGGTTGTATAGTTAGATCGGAAGAGCACACGTCTGAACTCC290.1899148657498363Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTCACAGTGGCTAAGTTCTGCAGATCGGAAGAGCACACGTCTGAACTCCA280.183366077275704Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GCACTGGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGT250.16371971185330714No Hit
AGCTGGTGTTGTGAATCAGGCCGTAGATCGGAAGAGCACACGTCTGAACT230.15062213490504256Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TACCCTGTAGATCCGAATTTGTAGATCGGAAGAGCACACGTCTGAACTCC230.15062213490504256Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGAGGTAGTAGATTGTATAGTAGATCGGAAGAGCACACGTCTGAACTCCA230.15062213490504256Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGAAAATCTTTCTTCTGAGGCGGAAAGATCGGAAGAGCACACGTCTGAAC220.1440733464309103Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTTGGCACTAGCACATTTTTGCTAGATCGGAAGAGCACACGTCTGAACTC210.137524557956778Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGAAATGATGGCAAATCATCTTTCGGGACTGACCTGAAATGAAGAGAATA200.13097576948264572No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTGGGGGT190.12442698100851343TruSeq Adapter, Index 8 (97% over 45bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATTTGGGGGG190.12442698100851343TruSeq Adapter, Index 8 (97% over 43bp)
TGTAAACATCCTCGACTGGAAGAGATCGGAAGAGCACACGTCTGAACTCC180.11787819253438114Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTGGGGGGGCGGG180.11787819253438114TruSeq Adapter, Index 8 (97% over 41bp)
TGAGATGAAGCACTGTAGCTCTAGATCGGAAGAGCACACGTCTGAACTCC180.11787819253438114Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTAGGGGGGGGGG180.11787819253438114TruSeq Adapter, Index 8 (97% over 41bp)
CTTGTCGTTACGATCGGCTGAGATTCTTCCTTGGTTCTACGATCCAAGAT170.11132940406024885No Hit
TGAGGTAGTAGTTTGTGCTGTAGATCGGAAGAGCACACGTCTGAACTCCA170.11132940406024885Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTAGGGTG170.11132940406024885TruSeq Adapter, Index 8 (97% over 45bp)
GGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTGGGGGGGGGGT170.11132940406024885TruSeq Adapter, Index 8 (97% over 41bp)
TGAGATGAAGCACTGTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCA160.10478061558611657Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ATGGGTGACCGCCTGGGAATACCCCGTGTCGTAGGCTTAGATCGGAAGAG160.10478061558611657No Hit
TCCCGTAGATCCGAACTTGTGGAGATCGGAAGAGCACACGTCTGAACTCC160.10478061558611657Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)