FastQCFastQC Report
Wed 30 Apr 2025
2F2_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename2F2_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences106837
Total Bases16 Mbp
Sequences flagged as poor quality0
Sequence length150
%GC70

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCATCTAGGGGG40833.8217097073111375TruSeq Adapter, Index 14 (97% over 43bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCATCTGGGGGG31402.939056693842021TruSeq Adapter, Index 14 (97% over 43bp)
TGAAAATCTTTGCTCTGAAGTGGAAAGATCGGAAGAGCACACGTCTGAAC30522.8566882259891235Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GATCGGCTCATTAATTCATGGAAGATCGGAAGAGCACACGTCTGAACTCC5490.5138669187640985Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCATCTGGGGG4830.4520905678744256TruSeq Adapter, Index 14 (97% over 43bp)
CAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCATCTAGGGG4200.39312223293428306TruSeq Adapter, Index 14 (97% over 43bp)
CTCGGGCTGAGACTTGAAGCGGAGATCGGAAGAGCACACGTCTGAACTCC4070.38095416381965047Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTCGGGCTGAGACTTGAAGCGGGTAGATCGGAAGAGCACACGTCTGAACT3820.3575540309068956Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTTGCGAGCTGTTGCCAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA3730.3491299830583038Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TGAAATGCGTAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTT3550.3322818873611202TruSeq Adapter, Index 14 (100% over 37bp)
GTGTCTTCACGAGCTTGCTCTGGAAGATCGGAAGAGCACACGTCTGAACT3190.2985856959667531Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GCACTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT3060.2864176268521205No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCATCTGGGGGGGGGGG2950.27612156837050833TruSeq Adapter, Index 14 (97% over 39bp)
GTGGAGCCGGCCTGGGCTGAGATCGGAAGAGCACACGTCTGAACTCCAGT2820.26395349925587575Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GTATTGTAAGTGCTGAGAGTAGATCGGAAGAGCACACGTCTGAACTCCAG2510.23493733444405965Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CATCTTGCTGAAGAACGTATCTGCAAGATCGGAAGAGCACACGTCTGAAC2310.2162172281138557Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCATCTAGTGGG2280.21340921216432507TruSeq Adapter, Index 14 (97% over 43bp)
GTGACCTCTGGCAGCATGAATGTGCACAACTCTGCCAGATCGGAAGAGCA2260.21153720153130473No Hit
CGGGCTGAGACTTGAAGCGGGTAGATCGGAAGAGCACACGTCTGAACTCC2210.20685717494875372Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCATCTAGGGGGGGGGG2080.19468910583412113TruSeq Adapter, Index 14 (97% over 39bp)
TTGCGAGCTGTTGCCAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC2010.18813706861854976Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GTGTGCTCAGGATCTGTGACAGATCGGAAGAGCACACGTCTGAACTCCAG1890.17690500482042737Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GATAAATTGTAAACTCCAAGATCGGAAGAGCACACGTCTGAACTCCAGTC1830.1712889729213662Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TGATCGGCTCATTAATTCATGGAAGATCGGAAGAGCACACGTCTGAACTC1690.15818489849022344Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CTCGGGCTGAGACTTGAAGCGAGATCGGAAGAGCACACGTCTGAACTCCA1690.15818489849022344Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGGTCTGTGATGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGT1580.14788884000861124TruSeq Adapter, Index 13 (100% over 36bp)
GAGACTTGAAGCGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAG1560.14601682937559085TruSeq Adapter, Index 13 (100% over 35bp)
TGAAAATCTTTGCTCTGAAGTGGAAGATCGGAAGAGCACACGTCTGAACT1490.13946479216001947Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GGAGCCGGCCTGGGCTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1450.13572077089397866Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCATCTAGGGTG1430.1338487602609583TruSeq Adapter, Index 14 (97% over 43bp)
TGAGGTAGTAGTTTGTGCTGTTAGATCGGAAGAGCACACGTCTGAACTCC1420.1329127549444481Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCATCTGGTGGG1400.13104074431142768TruSeq Adapter, Index 14 (97% over 43bp)
TCTCGGGCTGAGACTTGAAGCGGGTAGATCGGAAGAGCACACGTCTGAAC1400.13104074431142768Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGTGTGCTCAGGATCTGTGACAGATCGGAAGAGCACACGTCTGAACTCCA1340.12542471241236652Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GTATAATTGTGAGGTGTGTCTTTTCTTAAAGCAGATCGGAAGAGCACACG1290.1207446858298155No Hit
GGGGCTTGCGGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAG1290.1207446858298155TruSeq Adapter, Index 13 (100% over 35bp)
ACTAACTTGACCTCATTAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1260.11793666988028492Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GATCGGCTCATTAATTCATGAGATCGGAAGAGCACACGTCTGAACTCCAG1250.11700066456377471Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TTGACCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCAT1230.11512865393075433TruSeq Adapter, Index 14 (97% over 41bp)
GAGACTTGAAGCGGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC1180.11044862734820335Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACAGATCGGAAGAGCACACG1160.10857661671518294No Hit
CAGGTCTGTGATGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAG1140.10670460608216253TruSeq Adapter, Index 13 (100% over 35bp)
TCTCGGGCTGAGACTTGAAGCGGAGATCGGAAGAGCACACGTCTGAACTC1130.10576860076565237Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GTGTGCTCAGGATCTGTGAAGATCGGAAGAGCACACGTCTGAACTCCAGT1100.10296058481612175Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GTGATAGATCTTTTGGAGAAGATCGGAAGAGCACACGTCTGAACTCCAGT1090.10202457949961155Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TGAGGTAGTAGGTTGTATGGTTAGATCGGAAGAGCACACGTCTGAACTCC1080.10108857418310135Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATC1070.10015256886659117No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)