FastQCFastQC Report
Wed 30 Apr 2025
2E1_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename2E1_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16734587
Total Bases2.5 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC69

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCCACTTCAGAGCAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA344890020.609412111574667Illumina DpnII expression Sequencing Primer (100% over 25bp)
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA14422388.618306504964837Illumina RNA PCR Primer (100% over 45bp)
ACAAATCTTAGAACAAAGGCTTAATCTCAGAAGATCGTAGCAAGATCGTC1342860.8024458565962816No Hit
TCCACTTCAGAGCAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAAC1080720.6458002220192228Illumina DpnII expression Sequencing Primer (100% over 26bp)
GCTTTAAGAAAAGACACACCTCACAATTATACGATCGTCGGACTGTAGAA961200.5743792780783894No Hit
AATTCAAATCCCTTCCAAGATCGTCGGACTGTAGAACTCTGAACGTGTAG695010.4153135060936969Illumina DpnII expression Sequencing Primer (96% over 30bp)
ACAAATCTTAGAACAAAGGCTTAATCTCAGAAGATCGTAGCAACGATCGT691030.41293519822150376No Hit
TCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATCATT564970.33760618054093594Illumina RNA PCR Primer (100% over 50bp)
GAGGTCAAGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGG508590.3039154775675073Illumina RNA PCR Primer (100% over 37bp)
CTGGCTGCGTTCTTCATCGATACGTGAGCCAAGAGATCCACCGTCAAAAG370630.22147543886204066No Hit
GGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGT352100.2104025632661266Illumina RNA PCR Primer (100% over 44bp)
TGGCAACAGCTCGCAAGGATCGTCGGACTGTAGAACTCTGAACGTGTAGA347040.20737888541856453Illumina DpnII expression Sequencing Primer (96% over 30bp)
TGTCTTTCACCTACAGCATTAATAGATCGTCGGACTGTAGAACTCTGAAC336970.20136140796304086Illumina DpnII expression Sequencing Primer (100% over 26bp)
TCCATGAATTAATGAGCCGATCGATCGTCGGACTGTAGAACTCTGAACGT336900.2013195784276003Illumina DpnII expression Sequencing Primer (96% over 28bp)
CAGCCCAGGCCGGCTCCACGATCGTCGGACTGTAGAACTCTGAACGTGTA326440.19506905070319333Illumina DpnII expression Sequencing Primer (96% over 30bp)
GCTGAGTTCATCAACAGAATATTTAAACAAGATCGTCGGACTGTAGAACT305450.18252616571893887No Hit
ACCCGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCTGAAC289510.1730009829343264Illumina DpnII expression Sequencing Primer (100% over 26bp)
CCGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCTGAACGT273480.16342201931843314Illumina DpnII expression Sequencing Primer (96% over 28bp)
TTTTCCTCCAATCCTCACGGCATAAACAAGATCGTCGGACTGTAGAACTC270020.16135444513808436Illumina DpnII expression Sequencing Primer (100% over 21bp)
ACAAATCTTAGAACAAAGGCTTAATCTCAGAAGATCGTAGCGATCGTCGG263160.15725515066490736No Hit
GTGGATAACCCTCCTCCGTCCGGACCTCGATCGTCGGACTGTAGAACTCT256800.1534546385877345Illumina DpnII expression Sequencing Primer (100% over 22bp)
TACCACTTCAGAGCAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA256550.15330524738973242Illumina DpnII expression Sequencing Primer (100% over 25bp)
GGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAAC249670.14919400162071522No Hit
TATCATCAGAGGACAATTTCACATTCCTCACTAACAGTCAGATCGTCGGA245970.14698301189028448No Hit
AAACGCTCTACTGAACTAGATCGTCGGACTGTAGAACTCTGAACGTGTAG243980.14579385795418792Illumina DpnII expression Sequencing Primer (96% over 30bp)
CACGTCGACGAGTTTTTTAGATCGTCGGACTGTAGAACTCTGAACGTGTA242600.14496921854121647Illumina DpnII expression Sequencing Primer (96% over 30bp)
TTTTTTTGGTCTTTCACCTACAGCATTAATAGATCGTCGGACTGTAGAAC233320.1394238172713793No Hit
CTACGCATTTCAGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCG209980.12547665502590533Illumina RNA PCR Primer (100% over 33bp)
AGAGCAGGGAAATTCGTGATGGATCGTCGGACTGTAGAACTCTGAACGTG208340.1244966487670117Illumina DpnII expression Sequencing Primer (96% over 29bp)
ACCCGCTTCAAGTCTCAGCCCGAGAGATCGTCGGACTGTAGAACTCTGAA196630.11749916505259436Illumina DpnII expression Sequencing Primer (100% over 25bp)
ACACATCCACTAGCGGTAGGTTGCCAGGATCGTCGGACTGTAGAACTCTG189040.11296364828125129Illumina DpnII expression Sequencing Primer (100% over 23bp)
TTTTTTTTTCCTTCACGAGTAAACATTCTAGATCGTCGGACTGTAGAACT188740.11278437884364878No Hit
GCTTTAAGAAAAGACACACCTCACAATTATACTGATCGTCGGACTGTAGA186340.11135022334282883No Hit
AGTAGATAACCCTCCCCAAGTCCGGACCTCGATCGTCGGACTGTAGAACT183570.1096949688689658No Hit
AATGAGGTCAAGTTAGTGATCGTCGGACTGTAGAACTCTGAACGTGTAGA182990.10934838128960099Illumina DpnII expression Sequencing Primer (96% over 30bp)
AATGAGGTCAAGTTAGTGGATCGTCGGACTGTAGAACTCTGAACGTGTAG179470.10724495322173173Illumina DpnII expression Sequencing Primer (96% over 30bp)
TTTTTTGAGCCAAGACGCTATCACTAGATCGTCGGACTGTAGAACTCTGA177350.10597811586267411Illumina DpnII expression Sequencing Primer (100% over 24bp)
ATCATCAGAGGACAATTTCACATTCCTCACTAACAGTCAGATCGTCGGAC175820.1050638417309014No Hit
TCCCACTTCAGAGCAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA175560.10490847488497923Illumina DpnII expression Sequencing Primer (100% over 25bp)
GTCAAGTAGCTTCTGCTCAGCTCAAACATCAAGGATCGTCGGACTGTAGA174090.10403005464072702No Hit
ACCCGCTTCAAGTCTCGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT172310.10296638931095223Illumina DpnII expression Sequencing Primer (96% over 30bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)