FastQCFastQC Report
Wed 30 Apr 2025
2E1_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename2E1_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16734587
Total Bases2.5 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC71

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAAAATCTTTGCTCTGAAGTGGAAAGATCGGAAGAGCACACGTCTGAAC340322420.336468417176953Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTGGGGGG14197898.484159184806892TruSeq Adapter, Index 5 (97% over 45bp)
TTGCTACGATCTTCTGAGATTAAGCCTTTGTTCTAAGATTTGTAGATCGG1331770.7958188630529096No Hit
TGAAAATCTTTGCTCTGAAGTGGAAGATCGGAAGAGCACACGTCTGAACT1060960.6339923417291385Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GTATAATTGTGAGGTGTGTCTTTTCTTAAAGCAGATCGGAAGAGCACACG949120.5671606953909291No Hit
GTTGCTACGATCTTCTGAGATTAAGCCTTTGTTCTAAGATTTGTAGATCG682940.40810089905415653No Hit
TTGGAAGGGATTTGAATTAGATCGGAAGAGCACACGTCTGAACTCCAGTC682860.40805309387079586Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TTGACCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGAT504850.30168058524539626TruSeq Adapter, Index 5 (100% over 41bp)
GGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTGGGGGGGGGGG450300.2690834258413428TruSeq Adapter, Index 5 (97% over 41bp)
AACTTTTGACGGTGGATCTCTTGGCTCACGTATCGATGAAGAACGCAGCC423930.2533256422760837No Hit
CAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTGGGGG353780.21140647211670055TruSeq Adapter, Index 5 (97% over 45bp)
CTTGCGAGCTGTTGCCAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA343140.20504838272973214Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TATTAATGCTGTAGGTGAAAGACAAGATCGGAAGAGCACACGTCTGAACT333800.1994671275723745Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GATCGGCTCATTAATTCATGGAAGATCGGAAGAGCACACGTCTGAACTCC332860.1989054166678867Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GTGGAGCCGGCCTGGGCTGAGATCGGAAGAGCACACGTCTGAACTCCAGT319160.19071877901737283Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TTGTTTAAATATTCTGTTGATGAACTCAGCAGATCGGAAGAGCACACGTC302460.180739446990834No Hit
CTCGGGCTGAGACTTGAAGCGGGTAGATCGGAAGAGCACACGTCTGAACT287950.1720687818587934Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTCGGGCTGAGACTTGAAGCGGAGATCGGAAGAGCACACGTCTGAACTCC269610.161109443573361Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTGTTTATGCCGTGAGGATTGGAGGAAAAAGATCGGAAGAGCACACGTCT265440.15861759839068632Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCTACGATCTTCTGAGATTAAGCCTTTGTTCTAAGATTTGTAGATCGGAA260360.15558196924728407No Hit
GAGGTCCGGACGGAGGAGGGTTATCCACAGATCGGAAGAGCACACGTCTG256080.1530243919374885Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGAAAATCTTTGCTCTGAAGTGGTAAGATCGGAAGAGCACACGTCTGAAC253050.15121377061770333Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCACTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT251490.15028156954217034No Hit
TGACTGTTAGTGAGGAATGTGAAATTGTCCTCTGATGATAAGATCGGAAG241830.14450909365137005No Hit
TAGTTCAGTAGAGCGTTTAGATCGGAAGAGCACACGTCTGAACTCCAGTC240180.14352311174455634Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TAAAAAACTCGTCGACGTGAGATCGGAAGAGCACACGTCTGAACTCCAGT238590.14257298372526314Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TATTAATGCTGTAGGTGAAAGACCAAAAAAAAGATCGGAAGAGCACACGT232430.13889198460649194No Hit
CATCACGAATTTCCCTGCTCTAGATCGGAAGAGCACACGTCTGAACTCCA206730.12353456945187831Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGAAATGCGTAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAG205640.12288322382858925TruSeq Adapter, Index 5 (100% over 37bp)
TCTCGGGCTGAGACTTGAAGCGGGTAGATCGGAAGAGCACACGTCTGAAC195820.11701513757106763Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TAGAATGTTTACTCGTGAAGGAAAAAAAAAAGATCGGAAGAGCACACGTC190410.11378231204630268No Hit
CTGGCAACCTACCGCTAGTGGATGTGTAGATCGGAAGAGCACACGTCTGA187950.11231230265796222Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGTATAATTGTGAGGTGTGTCTTTTCTTAAAGCAGATCGGAAGAGCACAC184510.11025667977345363No Hit
ACTAACTTGACCTCATTAGATCGGAAGAGCACACGTCTGAACTCCAGTCA182130.10883447556847385Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GAGGTCCGGACTTGGGGAGGGTTATCTACTAGATCGGAAGAGCACACGTC181710.10858349835583035No Hit
CACTAACTTGACCTCATTAGATCGGAAGAGCACACGTCTGAACTCCAGTC178220.10649799723172135Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TAGTGATAGCGTCTTGGCTCAAAAAAAGATCGGAAGAGCACACGTCTGAA175050.10460371684105499Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CTTGATGTTTGAGCTGAGCAGAAGCTACTTGACAGATCGGAAGAGCACAC173640.10376115048432327No Hit
TGACTGTTAGTGAGGAATGTGAAATTGTCCTCTGATGATAGATCGGAAGA173300.10355797845504044No Hit
TGAAAATCTTTGCTCTGAAGTGGGAAGATCGGAAGAGCACACGTCTGAAC170290.10175930843109543Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GAGACTTGAAGCGGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC169210.10111393845572646Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)