FastQCFastQC Report
Wed 30 Apr 2025
2D3_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename2D3_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3037939
Total Bases455.6 Mbp
Sequences flagged as poor quality0
Sequence length150
%GC77

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA173317657.05104677875362Illumina RNA PCR Primer (100% over 45bp)
TCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATCATT45988815.138157810278614Illumina RNA PCR Primer (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG114800.37788777194012124No Hit
CATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA106460.3504349494838441Illumina RNA PCR Primer (100% over 45bp)
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGTCGTA81500.2682739844348422Illumina RNA PCR Primer (97% over 45bp)
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCAGTA80260.264192269825036Illumina RNA PCR Primer (97% over 45bp)
CACGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCC58170.19147849907453704Illumina RNA PCR Primer (100% over 42bp)
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTTGCCGTA51740.17031283379949366Illumina RNA PCR Primer (97% over 45bp)
CACGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGC50870.16744905016196834Illumina RNA PCR Primer (100% over 41bp)
GGTCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA48770.16053646896794174Illumina RNA PCR Primer (100% over 45bp)
GATCGTCGGACTGTAAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTAT47000.1547101505329765Illumina RNA PCR Primer (100% over 35bp)
GACCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA44810.14750131585920587Illumina RNA PCR Primer (100% over 45bp)
GATCATCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA43960.14470336632829034Illumina RNA PCR Primer (100% over 45bp)
GATCGTCGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTAT42220.1389757990532397Illumina RNA PCR Primer (97% over 44bp)
GATCGTCGGACTGTAGACTCTGAACGTGTAGATCTCGGTGGTCGCCGTAT37870.12465688086561316Illumina RNA PCR Primer (97% over 35bp)
AGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGT36660.12067391741572164Illumina RNA PCR Primer (100% over 44bp)
GATCGTCGGACTGAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTAT34550.11372841916839015Illumina RNA PCR Primer (100% over 37bp)
TATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA33800.1112596401705235Illumina RNA PCR Primer (100% over 45bp)
AATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA31510.10372163496370401Illumina RNA PCR Primer (100% over 45bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)