FastQCFastQC Report
Wed 30 Apr 2025
2D3_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename2D3_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3037939
Total Bases455.6 Mbp
Sequences flagged as poor quality0
Sequence length150
%GC81

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCATCTGGGGGG131059543.140925476120486TruSeq Adapter, Index 20 (97% over 43bp)
GGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCATCTGGGGGGGGGGG2385077.850947632589069TruSeq Adapter, Index 20 (97% over 39bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCATCTAGGGGG2166307.130821257438019TruSeq Adapter, Index 20 (97% over 43bp)
GGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCATCTAGGGGGGGGGG284880.9377410145496667TruSeq Adapter, Index 20 (97% over 39bp)
AGATCGGAGAGCACACGTCTGAACTCCAGTCACGTGGCCATCTGGGGGGG160640.5287795442897306TruSeq Adapter, Index 20 (97% over 36bp)
AGATCGGAAGAGCACACGTCTAAACTCCAGTCACGTGGCCATCTGGGGGG155490.511827261837713TruSeq Adapter, Index 20 (97% over 39bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCATCTGGGGTG155270.5111030866650055TruSeq Adapter, Index 20 (97% over 43bp)
GGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCATCTGGGGGGGGGGT149850.4932620437737558TruSeq Adapter, Index 20 (97% over 39bp)
GGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCATCTGGGGGGGGGTG130560.42976504794862574TruSeq Adapter, Index 20 (97% over 39bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCATATGGGGGG110920.3651159552578245TruSeq Adapter, Index 20 (97% over 41bp)
AGATCGGAAAGCACACGTCTGAACTCCAGTCACGTGGCCATCTGGGGGGG98420.3239696386267137TruSeq Adapter, Index 20 (96% over 32bp)
AGATCGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCATCTGGGGGGG95680.3149503660211742TruSeq Adapter, Index 20 (97% over 35bp)
GGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCATCTGGGGGGGTGGG92770.30537150350945164TruSeq Adapter, Index 20 (97% over 39bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCATCGGGGGGG91310.3005656137269379TruSeq Adapter, Index 20 (97% over 42bp)
GGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCATCTGGGGGGGCGGG83010.2732444594838804TruSeq Adapter, Index 20 (97% over 39bp)
AGATCGGAAGGCACACGTCTGAACTCCAGTCACGTGGCCATCTGGGGGGG66960.22041258892953414TruSeq Adapter, Index 20 (96% over 33bp)
AGATCGGAAGACACACGTCTGAACTCCAGTCACGTGGCCATCTGGGGGGG64760.21317083720245864TruSeq Adapter, Index 20 (96% over 29bp)
AGATCGGAAGAGCACACGTCTGAACTCAGTCACGTGGCCATCTGGGGGGG63700.20968162955214042Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
GGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCATCTGGGGGGTGGGG63330.20846369857985955TruSeq Adapter, Index 20 (97% over 39bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCATCTGGTGGG62130.20451365218327294TruSeq Adapter, Index 20 (97% over 43bp)
AGATCGGAAGAGCAACGTCTGAACTCCAGTCACGTGGCCATCTGGGGGGG57630.18970097819607307TruSeq Adapter, Index 20 (96% over 29bp)
GGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCATCTGGGGGGGAGGG53350.17561247938158073TruSeq Adapter, Index 20 (97% over 39bp)
AGATCGGAAGAGCACAGTCTGAACTCCAGTCACGTGGCCATCTGGGGGGG52260.17202452057134787TruSeq Adapter, Index 20 (96% over 27bp)
AGATCGGAAGAGACACGTCTGAACTCCAGTCACGTGGCCATCTGGGGGGG51730.17027991674618878TruSeq Adapter, Index 20 (96% over 31bp)
AGATCGGAAAAGCACACGTCTGAACTCCAGTCACGTGGCCATCTGGGGGG51320.16893031756068835TruSeq Adapter, Index 20 (97% over 39bp)
AGATCGGAAGAGCACACATCTGAACTCCAGTCACGTGGCCATCTGGGGGG50670.16679070909587057TruSeq Adapter, Index 20 (97% over 39bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCATCTAGGGTG49620.16333441849885727TruSeq Adapter, Index 20 (97% over 43bp)
AGATCGGAAGAACACACGTCTGAACTCCAGTCACGTGGCCATCTGGGGGG48780.16056938602124665TruSeq Adapter, Index 20 (97% over 39bp)
AGATCAGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCATCTGGGGGG48760.16050355191463686TruSeq Adapter, Index 20 (97% over 39bp)
AGATCGGAAGAGCACCGTCTGAACTCCAGTCACGTGGCCATCTGGGGGGG47650.15684975899779421TruSeq Adapter, Index 20 (96% over 25bp)
AGATCGGAAGAGCCACGTCTGAACTCCAGTCACGTGGCCATCTGGGGGGG45500.14977259253724318TruSeq Adapter, Index 20 (96% over 27bp)
GTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCATCTGGG42720.14062165171848415TruSeq Adapter, Index 20 (97% over 43bp)
AGATCGGAAGAGCACACTCTGAACTCCAGTCACGTGGCCATCTGGGGGGG42000.13825162388053217RNA PCR Primer, Index 20 (95% over 24bp)
AGATCGAAAGAGCACACGTCTGAACTCCAGTCACGTGGCCATCTGGGGGG41570.13683619058842195TruSeq Adapter, Index 20 (97% over 39bp)
CGTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCATCTGG38020.1251506366651865TruSeq Adapter, Index 20 (97% over 43bp)
GGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCATCTGGGGTGGGGGG36950.12162851196156341TruSeq Adapter, Index 20 (97% over 39bp)
AGATCGGAAGAGCACACGTCTGAACTCCAATCACGTGGCCATCTGGGGGG36370.11971932286987987TruSeq Adapter, Index 20 (97% over 39bp)
AAATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCATCTGGGGGG35500.11685553923235457TruSeq Adapter, Index 20 (97% over 39bp)
AGATCGGAAGAGCACACGTTGAACTCCAGTCACGTGGCCATCTGGGGGGG34330.1130042439956826RNA PCR Primer, Index 20 (95% over 24bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCATCTGGGAGG32330.10642083333470488TruSeq Adapter, Index 20 (97% over 43bp)
TAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCATCTGGGGG31720.10441289308310668TruSeq Adapter, Index 20 (97% over 43bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)