FastQCFastQC Report
Wed 30 Apr 2025
2D1_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename2D1_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16065514
Total Bases2.4 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC68

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCCACTTCAGAGCAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA405365525.23202805711663Illumina DpnII expression Sequencing Primer (100% over 25bp)
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA9690436.031820706141117Illumina RNA PCR Primer (100% over 45bp)
AGCAGACAACTACTCACGCTTAAGCACACGATCGTCGGACTGTAGAACTC3223032.006179198499345Illumina DpnII expression Sequencing Primer (100% over 21bp)
ACAAATCTTAGAACAAAGGCTTAATCTCAGAAGATCGTAGCAACGATCGT1797011.1185512022833506No Hit
ACAAATCTTAGAACAAAGGCTTAATCTCAGAAGATCGTAGCAAGATCGTC1779581.1077018762051436No Hit
AGACAACTACTCACGCTTAAGCACACGATCGTCGGACTGTAGAACTCTGA1527630.950875272338003Illumina DpnII expression Sequencing Primer (100% over 24bp)
TCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATCATT1480790.9217196536631197Illumina RNA PCR Primer (100% over 50bp)
TCCACTTCAGAGCAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAAC1020100.6349625664015481Illumina DpnII expression Sequencing Primer (100% over 26bp)
GGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAAC719120.44761717552267544No Hit
ACAAATCTTAGAACAAAGGCTTAATCTCAGAAGATCGTAGCGATCGTCGG628480.3911981901108175No Hit
AAACACAGCAGACAACTACTCACGCTTAAGCACACGATCGTCGGACTGTA498360.31020482755796047No Hit
CTGGCTGCGTTCTTCATCGATACGTGAGCCAAGAGATCCACCGTCAAAAG495620.3084993110086612No Hit
GAGGTCAAGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGG412130.2565308523586609Illumina RNA PCR Primer (100% over 37bp)
GCTTTAAGAAAAGACACACCTCACAATTATACGATCGTCGGACTGTAGAA411140.25591462557624983No Hit
TTTATAGTGTTCAGTAGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT407080.2533874733170691Illumina DpnII expression Sequencing Primer (96% over 30bp)
AAACTAACCAGCCTAGACCTGAGATCGTCGGACTGTAGAACTCTGAACGT402090.25028144135319913Illumina DpnII expression Sequencing Primer (96% over 28bp)
AAGGATGATAACCCTCGACCGTCCGGACCTCGATCGTCGGACTGTAGAAC314400.1956986872626671No Hit
ACAAATCTTAGAACAAAGGCTTAATCTCAGAAGATCGTAGCAGATCGTCG310980.19356990383251976No Hit
AGTAGATAACCCTCCCCAAGTCCGGACCTCGATCGTCGGACTGTAGAACT264770.16480642947371618No Hit
ACCCGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCTGAAC246940.15370812287736327Illumina DpnII expression Sequencing Primer (100% over 26bp)
TTCCACTTCAGAGAAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA245250.1526561801881969Illumina DpnII expression Sequencing Primer (100% over 25bp)
TCCCACTTCAGAGCAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA241150.15010412987720156Illumina DpnII expression Sequencing Primer (100% over 25bp)
TTTTTTTGGTCTTTCACCTACAGCATTAATAGATCGTCGGACTGTAGAAC234430.14592125717235066No Hit
AGCCGACAGTACCCTAAGCACTTCCCAACTAGTCCCCGATCGTCGGACTG233900.14559135798580736No Hit
TACCACTTCAGAGCAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA225620.1404374612601875Illumina DpnII expression Sequencing Primer (100% over 25bp)
GCAGACAACTACTCACGCTTAAGCACACGATCGTCGGACTGTAGAACTCT221120.13763643043104629Illumina DpnII expression Sequencing Primer (100% over 22bp)
ACAAGTTCGGATCTACGGGAGATCGTCGGACTGTAGAACTCTGAACGTGT219030.13633550722373403Illumina DpnII expression Sequencing Primer (96% over 30bp)
AATTCAGAAAAACTGAACAGTCATCAGTGCTGATCGTCGGACTGTAGAAC216580.13481050155009047No Hit
ACAAATCTTAGAACAAAGGCTTAATCTCAGAAGATCGTAGCAACAGATCG211830.13185385789710805No Hit
TGTCTTTCACCTACAGCATTAATAGATCGTCGGACTGTAGAACTCTGAAC211030.13135589686081628Illumina DpnII expression Sequencing Primer (100% over 26bp)
GGCAGACAACTACTCACGCTTAAGCACACGATCGTCGGACTGTAGAACTC203440.12663149152899808Illumina DpnII expression Sequencing Primer (100% over 21bp)
CCAAGACCACAAAGGTACCAGACATCGGAGATCGTCGGACTGTAGAACTC203190.1264758787051569Illumina DpnII expression Sequencing Primer (100% over 21bp)
TTCCACTTCAGAGCAAGGATTTTCAGATCGTCGGACTGTAGAACTCTGAA194280.12092983766345726Illumina DpnII expression Sequencing Primer (100% over 25bp)
GTCAAGTAGCTTCTGCTCAGCTCAAACATCAAGGATCGTCGGACTGTAGA188600.11739431430578567No Hit
AATGAGGTCAATGAAGGCGGGGAGGGGCGTACCGAAGGATCGTCGGACTG187440.1166722708031626No Hit
AGGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAA179760.11189184485476157No Hit
TGGCAACAGCTCGCAAGGATCGTCGGACTGTAGAACTCTGAACGTGTAGA176860.1100867360982039Illumina DpnII expression Sequencing Primer (96% over 30bp)
TTCCACTTCAGAGTAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA176400.10980040850233613Illumina DpnII expression Sequencing Primer (100% over 25bp)
CCGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCTGAACGT175460.10921530428469328Illumina DpnII expression Sequencing Primer (96% over 28bp)
CTCCACTTCAGAGCAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA175410.10918418171992506Illumina DpnII expression Sequencing Primer (100% over 25bp)
TTCCACCTCAGAGCAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA171630.10683131582344642Illumina DpnII expression Sequencing Primer (100% over 25bp)
CAGCCCAGGCCGGCTCCACGATCGTCGGACTGTAGAACTCTGAACGTGTA169260.10535610625343203Illumina DpnII expression Sequencing Primer (96% over 30bp)
CCACTTCAGAGCAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAACG168880.10511957476119344Illumina Small RNA Adapter 1 (100% over 26bp)
TTTTCCTCCAATCCTCACGGCATAAACAAGATCGTCGGACTGTAGAACTC166070.10337048662121859Illumina DpnII expression Sequencing Primer (100% over 21bp)
ACTTCAGAGCAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAACGTG161730.10066904799933571Illumina DpnII expression Sequencing Primer (96% over 29bp)
GTGGATAACCCTCCTCCGTCCGGACCTCGATCGTCGGACTGTAGAACTCT161350.10043251650709714Illumina DpnII expression Sequencing Primer (100% over 22bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)