FastQCFastQC Report
Wed 30 Apr 2025
2D1_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename2D1_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16065514
Total Bases2.4 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC71

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAAAATCTTTGCTCTGAAGTGGAAAGATCGGAAGAGCACACGTCTGAAC397886124.76647183526154Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTGGGGGG8812685.485464081634736TruSeq Adapter, Index 4 (97% over 45bp)
GTGTGCTTAAGCGTGAGTAGTTGTCTGCTAGATCGGAAGAGCACACGTCT3190971.9862234099699518Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTTGCTACGATCTTCTGAGATTAAGCCTTTGTTCTAAGATTTGTAGATCG1776151.1055668682620425No Hit
TTGCTACGATCTTCTGAGATTAAGCCTTTGTTCTAAGATTTGTAGATCGG1764601.0983775558005802No Hit
GTGTGCTTAAGCGTGAGTAGTTGTCTAGATCGGAAGAGCACACGTCTGAA1509320.939478189119875Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTGGGGGGGGGGG1157520.7204998234105675TruSeq Adapter, Index 4 (97% over 41bp)
TGAAAATCTTTGCTCTGAAGTGGAAGATCGGAAGAGCACACGTCTGAACT996190.6200797559293776Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GCACTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT721900.44934759012378933No Hit
AACTTTTGACGGTGGATCTCTTGGCTCACGTATCGATGAAGAACGCAGCC673890.41946370343332934No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTAGGGGG672700.4187229863918453TruSeq Adapter, Index 4 (97% over 45bp)
GCTACGATCTTCTGAGATTAAGCCTTTGTTCTAAGATTTGTAGATCGGAA621130.3866231730898868No Hit
GTGTGCTTAAGCGTGAGTAGTTGTCTGCTGTGTTTAGATCGGAAGAGCAC493380.3071050201070442No Hit
GTATAATTGTGAGGTGTGTCTTTTCTTAAAGCAGATCGGAAGAGCACACG405360.2523168570890418No Hit
TTGACCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAAT403820.2513582820941801TruSeq Adapter, Index 4 (100% over 41bp)
TACTGAACACTATAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC398370.2479659225344424Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCAGGTCTAGGCTGGTTAGTTTAGATCGGAAGAGCACACGTCTGAACTCC395130.24594918033746072Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GAGGTCCGGACGGTCGAGGGTTATCATCCTTAGATCGGAAGAGCACACGT311600.1939558236356459No Hit
TGCTACGATCTTCTGAGATTAAGCCTTTGTTCTAAGATTTGTAGATCGGA307990.19170877445937928No Hit
GAGGTCCGGACTTGGGGAGGGTTATCTACTAGATCGGAAGAGCACACGTC261150.16255315578449592No Hit
TGAAAATCTTTTCTCTGAAGTGGAAAGATCGGAAGAGCACACGTCTGAAC245200.15262505762342868Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CTCGGGCTGAGACTTGAAGCGGGTAGATCGGAAGAGCACACGTCTGAACT244370.15210842304827596Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TGAAAATCTTTGCTCTGAAGTGGGAAGATCGGAAGAGCACACGTCTGAAC233150.14512451951428382Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TATTAATGCTGTAGGTGAAAGACCAAAAAAAAGATCGGAAGAGCACACGT232540.14474482422411136No Hit
GGGGACTAGTTGGGAAGTGCTTAGGGTACTGTCGGCTAGATCGGAAGAGC231430.1440539032862565No Hit
TGAAAATCTTTGCTCTGAAGTGGTAAGATCGGAAGAGCACACGTCTGAAC221140.1376488794569536Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GTGTGCTTAAGCGTGAGTAGTTGTCTGCAGATCGGAAGAGCACACGTCTG218190.13581264813562766Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCCGTAGATCCGAACTTGTAGATCGGAAGAGCACACGTCTGAACTCCAG216390.13469223580397116Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AGCACTGATGACTGTTCAGTTTTTCTGAATTAGATCGGAAGAGCACACGT215240.13397641681430172No Hit
TGTTGCTACGATCTTCTGAGATTAAGCCTTTGTTCTAAGATTTGTAGATC209990.13070854751363697No Hit
TATTAATGCTGTAGGTGAAAGACAAGATCGGAAGAGCACACGTCTGAACT207910.12941384881927837Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GTGTGCTTAAGCGTGAGTAGTTGTCTGCCAGATCGGAAGAGCACACGTCT203310.12655057286060065Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCGATGTCTGGTACCTTTGTGGTCTTGGAGATCGGAAGAGCACACGTCT198230.1233885202801479Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGAAAATCCTTGCTCTGAAGTGGAAAGATCGGAAGAGCACACGTCTGAAC192970.12011442646652948Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CTTGATGTTTGAGCTGAGCAGAAGCTACTTGACAGATCGGAAGAGCACAC187600.11677186301042096No Hit
CTTCGGTACGCCCCTCCCCGCCTTCATTGACCTCATTAGATCGGAAGAGC183740.11436920101031314No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACG179180.11153082310345003No Hit
CTTGCGAGCTGTTGCCAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA173130.10776499276649351Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CTCGGGCTGAGACTTGAAGCGGAGATCGGAAGAGCACACGTCTGAACTCC171440.10671305007732712Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGAAAATCTTTACTCTGAAGTGGAAAGATCGGAAGAGCACACGTCTGAAC170780.10630223222238641Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TGAAAATCTTTGCTCTGAGGTGGAAAGATCGGAAGAGCACACGTCTGAAC166490.10363191616527176Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GTGGAGCCGGCCTGGGCTGAGATCGGAAGAGCACACGTCTGAACTCCAGT164660.10249283029475434Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TGAAAATCTTTGCTCTGAAGTGGAGATCGGAAGAGCACACGTCTGAACTC163830.10197619571960163Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TTGTTTATGCCGTGAGGATTGGAGGAAAAAGATCGGAAGAGCACACGTCT163300.10164629653305833Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)