FastQCFastQC Report
Wed 30 Apr 2025
2B1_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename2B1_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17656021
Total Bases2.6 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC67

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCCACTTCAGAGCAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA217123512.297419673436048Illumina DpnII expression Sequencing Primer (100% over 25bp)
GGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAAC3078361.743518542484742No Hit
GCTGAGTTCATCAACAGAATATTTAAACAAGATCGTCGGACTGTAGAACT2018061.1429868598366528No Hit
ACAAATCTTAGAACAAAGGCTTAATCTCAGAAGATCGTAGCAAGATCGTC923300.5229377559077439No Hit
ACAAATCTTAGAACAAAGGCTTAATCTCAGAAGATCGTAGCAACGATCGT824030.46671330986749504No Hit
AAACTAACCAGCCTAGACCTGAGATCGTCGGACTGTAGAACTCTGAACGT807860.45755496099602505Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAA782770.44334451120102314No Hit
GCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAACT751020.4253619770841913No Hit
GGCGAGAATTCTACCACTGAACCACAGTGCGATCGTCGGACTGTAGAACT681720.38611191049217714No Hit
TCCACTTCAGAGCAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAAC539630.3056351145028656Illumina DpnII expression Sequencing Primer (100% over 26bp)
TTTTTTTGGTCTTTCACCTACAGCATTAATAGATCGTCGGACTGTAGAAC532640.3016761251020261No Hit
TTCCACTTCAGAGCCAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA501520.2840504097723944Illumina DpnII expression Sequencing Primer (100% over 25bp)
AGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAAC411700.23317824554014743No Hit
CGGAAATACTAACCACTATACTACAGCGGAGATCGTCGGACTGTAGAACT401610.2274634811546724No Hit
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA392320.22220181999103875Illumina RNA PCR Primer (100% over 45bp)
TCCCACTTCAGAGCAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA374010.21183142000114294Illumina DpnII expression Sequencing Primer (100% over 25bp)
GAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAACTCT370590.20989440372777085Illumina DpnII expression Sequencing Primer (100% over 22bp)
CATTGAAAATCTTCGGTCTTATCTATCCAGATCGTCGGACTGTAGAACTC341350.19333348096946645Illumina DpnII expression Sequencing Primer (100% over 21bp)
GGAAATACTAACCACTATACTACAGCGGAGATCGTCGGACTGTAGAACTC338040.1914587663890975Illumina DpnII expression Sequencing Primer (100% over 21bp)
ACAAGTTCGGATCTACGGGAGATCGTCGGACTGTAGAACTCTGAACGTGT285370.1616275830211122Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGCCTTCATAAATTCCATCATCAACACTGATTGATCGTCGGACTGTAGAA270650.15329048373922982No Hit
AGTCTCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAGA267150.15130815714367352No Hit
CCACTTCAGAGCAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAACG265490.15036796795835256Illumina Small RNA Adapter 1 (100% over 26bp)
GCCTGGCTATTACCACTTAATCAACTACAAGATCGTCGGACTGTAGAACT248190.14056961078603158No Hit
ACCCGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCTGAAC244810.1386552496737515Illumina DpnII expression Sequencing Primer (100% over 26bp)
ACTGAGTTCATCAACAGAATATTTAAACAAGATCGTCGGACTGTAGAACT232780.13184170997531097No Hit
TTCCACTTCAGAGCAAAGGTTTTCAGATCGTCGGACTGTAGAACTCTGAA230490.13054470200278986Illumina DpnII expression Sequencing Primer (100% over 25bp)
CCACAAGTTCGGATCTACGGGAGATCGTCGGACTGTAGAACTCTGAACGT223720.12671031598795673Illumina DpnII expression Sequencing Primer (96% over 28bp)
TTCCCACTTCAGAGCAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGA214240.12134104280913575Illumina DpnII expression Sequencing Primer (100% over 24bp)
TCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATCATT212800.12052545700982119Illumina RNA PCR Primer (100% over 50bp)
TCCGACACTCAGACAGACATGCTCCTGGGAGAACCCAAGAGCGCCATTTG210440.11918880250538896No Hit
AATGGAAGCCATACAGGTTGTCACGTTGAAGATCGTCGGACTGTAGAACT197940.11210906466411656No Hit
CCGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCTGAACGT196340.1112028582204337Illumina DpnII expression Sequencing Primer (96% over 28bp)
TCCGACACTCAGACAGACATGCTCCTGGGGATCGTCGGACTGTAGAACTC195470.11071010846668114Illumina DpnII expression Sequencing Primer (100% over 21bp)
GAAATACTAACCACTATACTACAGCGGAGATCGTCGGACTGTAGAACTCT195240.11057984129040173Illumina DpnII expression Sequencing Primer (100% over 22bp)
GCTTCCAATTTTCAGAAGTCAGTGCAAACAGATCGTCGGACTGTAGAACT191190.10828600622982948No Hit
CTGGCTGCGTTCTTCATCGATACGTGAGCCAAGAGATCCACCGTCAAAAG177510.10053794113634097No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)