FastQCFastQC Report
Wed 30 Apr 2025
2B1_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename2B1_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17656021
Total Bases2.6 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC65

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAAAATCTTTGCTCTGAAGTGGAAAGATCGGAAGAGCACACGTCTGAAC213922112.116099091635652Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCACTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT3105551.7589183882370778No Hit
TTGTTTAAATATTCTGTTGATGAACTCAGCAGATCGGAAGAGCACACGTC1992251.1283686171419938No Hit
TTGCTACGATCTTCTGAGATTAAGCCTTTGTTCTAAGATTTGTAGATCGG918390.520156834883692No Hit
GTTGCTACGATCTTCTGAGATTAAGCCTTTGTTCTAAGATTTGTAGATCG817250.46287326006238894No Hit
TCAGGTCTAGGCTGGTTAGTTTAGATCGGAAGAGCACACGTCTGAACTCC803170.4548986433579797Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCACTGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACG782650.44327654571774694No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTC750030.42480126184716255No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTC682790.38671793605139004No Hit
TATTAATGCTGTAGGTGAAAGACCAAAAAAAAGATCGGAAGAGCACACGT530640.3005433670474225No Hit
TGAAAATCTTTGCTCTGAAGTGGAAGATCGGAAGAGCACACGTCTGAACT529520.2999090225368445Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AACTTTTGACGGTGGATCTCTTGGCTCACGTATCGATGAAGAACGCAGCC522780.29609162789283044No Hit
TGAAAATCTTGGCTCTGAAGTGGAAAGATCGGAAGAGCACACGTCTGAAC490760.2779561714386271Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCACTGGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGT415470.23531349447307523No Hit
TCCGCTGTAGTATAGTGGTTAGTATTTCCGAGATCGGAAGAGCACACGTC389640.22068392419786997No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCAGATCGGAAGAGCACACGTCTG370420.20979811929312953Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGAAAATCTTTGCTCTGAAGTGGGAAGATCGGAAGAGCACACGTCTGAAC367150.20794605987385267Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TGGATAGATAAGACCGAAGATTTTCAATGAGATCGGAAGAGCACACGTCT334550.18948210358381426Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCGCTGTAGTATAGTGGTTAGTATTTCCAGATCGGAAGAGCACACGTCT328870.1862650707087401Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCCGTAGATCCGAACTTGTAGATCGGAAGAGCACACGTCTGAACTCCAG283580.16061376456224197Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AATCAGTGTTGATGATGGAATTTATGAAGGCTAGATCGGAAGAGCACACG269390.15257684616482955No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTAGATCGGAAGAGCACAC267360.1514270967394069No Hit
TGAAAATCTTTGCTCTGAAGTGGAGATCGGAAGAGCACACGTCTGAACTC259000.14669216807116392Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TTGTAGTTGATTAAGTGGTAATAGCCAGGCAGATCGGAAGAGCACACGTC246020.13934056829678668No Hit
CTCGGGCTGAGACTTGAAGCGGGTAGATCGGAAGAGCACACGTCTGAACT243880.13812851717836086Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTGTTTAAATATTCTGTTGATGAACTCAGTAGATCGGAAGAGCACACGTC230460.1305277106319708No Hit
TGAAAACCTTTGCTCTGAAGTGGAAAGATCGGAAGAGCACACGTCTGAAC227810.1290268062096211Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCCCGTAGATCCGAACTTGTGGAGATCGGAAGAGCACACGTCTGAACTCC220880.12510179955041967Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGAAAATCTTTGCTCTGAAGTGGGAAAGATCGGAAGAGCACACGTCTGAA210460.11920013008593498Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTCAACGTGACAACCTGTATGGCTTCCATTAGATCGGAAGAGCACACGTC198550.11245455587077066No Hit
CCCAGGAGCATGTCTGTCTGAGTGTCGGAAGATCGGAAGAGCACACGTCT194620.11022868629347461Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTCGGGCTGAGACTTGAAGCGGAGATCGGAAGAGCACACGTCTGAACTCC192300.10891468695013447Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCGCTGTAGTATAGTGGTTAGTATTTCAGATCGGAAGAGCACACGTCTG189460.10730617051259739Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGTTTGCACTGACTTCTGAAAATTGGAAGCAGATCGGAAGAGCACACGTC188790.10692669656430517No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)