FastQCFastQC Report
Wed 30 Apr 2025
2A3_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename2A3_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2432495
Total Bases364.8 Mbp
Sequences flagged as poor quality0
Sequence length150
%GC77

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA123758850.877309100327025Illumina RNA PCR Primer (100% over 45bp)
TCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATCATT32681913.435546630106126Illumina RNA PCR Primer (100% over 50bp)
CATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA90760.3731148471014329Illumina RNA PCR Primer (100% over 45bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG87360.35913742885391337No Hit
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGTCGTA56180.2309562815134255Illumina RNA PCR Primer (97% over 45bp)
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCAGTA55300.2273385967905381Illumina RNA PCR Primer (97% over 45bp)
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTTGCCGTA43130.17730766147515203Illumina RNA PCR Primer (97% over 45bp)
GGTCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA37830.15551933303048926Illumina RNA PCR Primer (100% over 45bp)
GATCGTCGGACTGTAAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTAT33660.13837644065044327Illumina RNA PCR Primer (100% over 35bp)
GATCGTCGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTAT31700.13031887013128496Illumina RNA PCR Primer (97% over 44bp)
GATCATCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA30780.12653674519372085Illumina RNA PCR Primer (100% over 45bp)
GACCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA30590.12575565417400653Illumina RNA PCR Primer (100% over 45bp)
CACGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCC30440.1251390033689689Illumina RNA PCR Primer (100% over 42bp)
TATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA29870.12279573030982592Illumina RNA PCR Primer (100% over 45bp)
AGTTCTGATAACCCACTACCATCGGACCAGCCGATCGTCGGACTGTAGAA29280.12037023714334459No Hit
GATCGTCGGACTGTAGACTCTGAACGTGTAGATCTCGGTGGTCGCCGTAT27880.11461482962966008Illumina RNA PCR Primer (97% over 35bp)
CACGGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGC27880.11461482962966008Illumina RNA PCR Primer (100% over 41bp)
AGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGT26300.10811944114993043Illumina RNA PCR Primer (100% over 44bp)
GCAGAGTACTAACCACTATACGATCACGGCGATCGTCGGACTGTAGAACT25210.10363844529999035No Hit
AGCGAGAATTCTACCACTGAACCACCAATGCGATCGTCGGACTGTAGAAC25210.10363844529999035No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)