FastQCFastQC Report
Wed 30 Apr 2025
2A3_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename2A3_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2432495
Total Bases364.8 Mbp
Sequences flagged as poor quality0
Sequence length150
%GC79

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAGAGATCTGGGGGG111020945.64075157400118TruSeq Adapter, Index 3 (97% over 37bp)
GGAAGAGCACACGTCTGAACTCCAGTCACGTAGAGATCTGGGGGGGGGGG2048258.420366742788783TruSeq Adapter, Index 3 (96% over 33bp)
AGATCGGAGAGCACACGTCTGAACTCCAGTCACGTAGAGATCTGGGGGGG140300.5767740529785262TruSeq Adapter, Index 3 (96% over 30bp)
GGAAGAGCACACGTCTGAACTCCAGTCACGTAGAGATCTGGGGGGGGGTG129070.5306074627080426TruSeq Adapter, Index 3 (96% over 33bp)
GGAAGAGCACACGTCTGAACTCCAGTCACGTAGAGATCTGGGGGGGGGGT117830.4843997623838898TruSeq Adapter, Index 3 (96% over 33bp)
AGATCGGAAAGCACACGTCTGAACTCCAGTCACGTAGAGATCTGGGGGGG97270.39987749204006584TruSeq Adapter, Index 18 (96% over 28bp)
AGATCGGAAGAGCACACGTCTAAACTCCAGTCACGTAGAGATCTGGGGGG95240.39153215114522333TruSeq Adapter, Index 18 (97% over 35bp)
AGATCGAAGAGCACACGTCTGAACTCCAGTCACGTAGAGATCTGGGGGGG86990.35761635686815385TruSeq Adapter, Index 18 (96% over 31bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAGAGATCTGGGGTG81030.3331147648813256TruSeq Adapter, Index 3 (97% over 37bp)
AGATCGGAAGGCACACGTCTGAACTCCAGTCACGTAGAGATCTGGGGGGG59940.24641366169303536TruSeq Adapter, Index 3 (96% over 27bp)
GGAAGAGCACACGTCTGAACTCCAGTCACGTAGAGATCTGGGGGGGTGGG59440.24435815900957658TruSeq Adapter, Index 3 (96% over 33bp)
AGATCGGAAGACACACGTCTGAACTCCAGTCACGTAGAGATCTGGGGGGG56730.23321733446523013TruSeq Adapter, Index 18 (96% over 25bp)
AGATCGGAAGAGCAACGTCTGAACTCCAGTCACGTAGAGATCTGGGGGGG54990.22606418512679366RNA PCR Primer, Index 17 (95% over 24bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAGAGATTTGGGGGG50080.20587914877522873TruSeq Adapter, Index 3 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCAGTCACGTAGAGATCTGGGGGGG48480.19930154018816074Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAGAGATCGGGGGGG48350.19876710949046145TruSeq Adapter, Index 3 (97% over 37bp)
AGATCGGAAGAGACACGTCTGAACTCCAGTCACGTAGAGATCTGGGGGGG48060.19757491793405535TruSeq Adapter, Index 3 (96% over 25bp)
AGATCGGAAGAGCACAGTCTGAACTCCAGTCACGTAGAGATCTGGGGGGG44450.18273418855948317RNA PCR Primer, Index 17 (96% over 25bp)
GGAAGAGCACACGTCTGAACTCCAGTCACGTAGAGATCTGGGGGGGCGGG42850.17615657997241516TruSeq Adapter, Index 3 (96% over 33bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAGAGATCTGGGAGG42630.17525215879169329TruSeq Adapter, Index 3 (97% over 37bp)
AGATCGGAAGAGCCACGTCTGAACTCCAGTCACGTAGAGATCTGGGGGGG42370.17418329739629473RNA PCR Primer, Index 17 (95% over 24bp)
AGATCGGAAAAGCACACGTCTGAACTCCAGTCACGTAGAGATCTGGGGGG42150.1732788762155729TruSeq Adapter, Index 18 (97% over 35bp)
AGATCGGAAGAACACACGTCTGAACTCCAGTCACGTAGAGATCTGGGGGG40990.1685101099899486TruSeq Adapter, Index 18 (97% over 35bp)
GGAAGAGCACACGTCTGAACTCCAGTCACGTAGAGATCTGGGGGGTGGGG39900.1640291141400085TruSeq Adapter, Index 3 (96% over 33bp)
AGATCGGAAGAGCACCGTCTGAACTCCAGTCACGTAGAGATCTGGGGGGG38140.1567937446942337RNA PCR Primer, Index 17 (95% over 24bp)
GGAAGAGCACACGTCTGAACTCCAGTCACGTAGAGATCTGGGGGGGAGGG37710.15502601238645916TruSeq Adapter, Index 3 (96% over 33bp)
AGATCGGAAGAGCACACATCTGAACTCCAGTCACGTAGAGATCTGGGGGG37600.15457380179609823TruSeq Adapter, Index 18 (97% over 35bp)
AGATCGGAAGAGCACACTCTGAACTCCAGTCACGTAGAGATCTGGGGGGG34980.14380296773477438RNA PCR Primer, Index 17 (95% over 24bp)
AGATCAGAAGAGCACACGTCTGAACTCCAGTCACGTAGAGATCTGGGGGG34060.14002084279721028TruSeq Adapter, Index 18 (97% over 35bp)
AAATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAGAGATCTGGGGGG31570.12978443943358567TruSeq Adapter, Index 18 (97% over 35bp)
AGATCGAAAGAGCACACGTCTGAACTCCAGTCACGTAGAGATCTGGGGGG30140.12390570175889364TruSeq Adapter, Index 18 (97% over 35bp)
AGATCGGAAGAGCACACGTTGAACTCCAGTCACGTAGAGATCTGGGGGGG29370.12074022762636717RNA PCR Primer, Index 17 (95% over 24bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTAGATCGGAAGAGCACACG27210.11186045603382534No Hit
GTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAGAGATCTGGG27140.11157268565814113TruSeq Adapter, Index 3 (97% over 37bp)
AGATAGGAAGAGCACACGTCTGAACTCCAGTCACGTAGAGATCTGGGGGG26060.10713279986187023TruSeq Adapter, Index 18 (97% over 35bp)
CGTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAGAGATCTGG24800.10195293309955417TruSeq Adapter, Index 3 (97% over 37bp)
TAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAGAGATCTGGGGG24510.10076074154314808TruSeq Adapter, Index 3 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAATCACGTAGAGATCTGGGGGG24370.10018520079177963TruSeq Adapter, Index 18 (97% over 35bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)