FastQCFastQC Report
Wed 30 Apr 2025
1H3_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1H3_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1881714
Total Bases282.2 Mbp
Sequences flagged as poor quality0
Sequence length150
%GC68

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA20098810.681113070317807Illumina RNA PCR Primer (100% over 45bp)
AGCAGACAACTACTCACGCTTAAGCACACGATCGTCGGACTGTAGAACTC1198406.3686617626270525Illumina DpnII expression Sequencing Primer (100% over 21bp)
AGCCGACAGTACCCTAAGCACTTCCCAACTAGTCCCCGATCGTCGGACTG706043.752111107213955No Hit
TCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATCATT408872.1728594249710635Illumina RNA PCR Primer (100% over 50bp)
TTCCACTTCAGAGCAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA341031.812337050157463Illumina DpnII expression Sequencing Primer (100% over 25bp)
AAACACAGCAGACAACTACTCACGCTTAAGCACACGATCGTCGGACTGTA327921.7426665263690446No Hit
AGACAACTACTCACGCTTAAGCACACGATCGTCGGACTGTAGAACTCTGA312781.6622079657163626Illumina DpnII expression Sequencing Primer (100% over 24bp)
ACAAATCTTAGAACAAAGGCTTAATCTCAGAAGATCGTAGCAACGATCGT153030.8132479218414701No Hit
GGCAGACAACTACTCACGCTTAAGCACACGATCGTCGGACTGTAGAACTC128330.6819846161531455Illumina DpnII expression Sequencing Primer (100% over 21bp)
ACAAATCTTAGAACAAAGGCTTAATCTCAGAAGATCGTAGCAAGATCGTC123910.6584953930299716No Hit
TAAGAAACACAGCAGACAACTACTCACGCTTAAGCACACGATCGTCGGAC121310.6446782029575164No Hit
GGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAAC99870.5307395278985011No Hit
GGCCGACAGTACCCTAAGCACTTCCCAACTAGTCCCCGATCGTCGGACTG71750.3813013029610238No Hit
GCTTTAAGAAAAGACACACCTCACAATTATACGATCGTCGGACTGTAGAA62160.33033712880916016No Hit
TATCATCAGAGGACAATTTCACATTCCTCACTAACAGTCAGATCGTCGGA57400.30504104236881907No Hit
ACAAATCTTAGAACAAAGGCTTAATCTCAGAAGATCGTAGCGATCGTCGG55780.29643187009290467No Hit
GGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGT53780.28580326234486214Illumina RNA PCR Primer (100% over 44bp)
GCAGACAACTACTCACGCTTAAGCACACGATCGTCGGACTGTAGAACTCT50000.26571519370106195Illumina DpnII expression Sequencing Primer (100% over 22bp)
ACCACCAAAGAGTCTAGAATTGTACATGATCGTCGGACTGTAGAACTCTG49870.26502433419743915Illumina DpnII expression Sequencing Primer (100% over 23bp)
ACTCTCAGCACTTACAATACGATCGTCGGACTGTAGAACTCTGAACGTGT44430.2361145211227636Illumina DpnII expression Sequencing Primer (96% over 30bp)
TATCATCAGAGGACAATTTCACATTCCTCACTAACAGTCGATCGTCGGAC42440.22553905641346134No Hit
ACAAATCTTAGAACAAAGGCTTAATCTCAGAAGATCGTAGCAGATCGTCG40800.2168235980600665No Hit
GGCAGAGTTGTGCACATTCATGCTGCCAGAGGTCACGATCGTCGGACTGT40330.21432587523927651No Hit
GCTGAGTTCATCAACAGAATATTTAAACAAGATCGTCGGACTGTAGAACT39090.2077361384354902No Hit
CTGGCTGCGTTCTTCATCGATACGTGAGCCAAGAGATCCACCGTCAAAAG36360.1932280888594122No Hit
AGTAGATAACCCTCCCCAAGTCCGGACCTCGATCGTCGGACTGTAGAACT33630.17872003928333424No Hit
GAGGTCAAGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGG32110.17064229739482195Illumina RNA PCR Primer (100% over 37bp)
ACTCAGTTAAGAGCACACACAGTCTTTGCGAAGAAGAACGATCGTCGGAC31960.1698451518137188No Hit
AAAGTAAACGTCACAGATCCTGAGCACACGATCGTCGGACTGTAGAACTC29770.15820682632961225Illumina DpnII expression Sequencing Primer (100% over 21bp)
AAGGTAAATGTCCCAAATACCGCACCACACAGTCAAGGATCGTCGGACTG28710.15257366422314975No Hit
AAGCCTACAACACGGGGTATTCCCAGATCGTCGGACTGTAGAACTCTGAA27770.14757821858156978Illumina DpnII expression Sequencing Primer (100% over 25bp)
ACAAATCTTAGAACAAAGGCTTAATCTCAGAAGATCGTAGCAACAGATCG26720.14199819951384748No Hit
GGACAACTACTCACGCTTAAGCACACGATCGTCGGACTGTAGAACTCTGA25900.13764047033715007Illumina DpnII expression Sequencing Primer (100% over 24bp)
GTCTCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAGAA24040.12775586513147055No Hit
GCCCAAGGCTCATAGCAGCCTACAGCAATCTCGATCGTCGGACTGTAGAA23240.12350442203225356No Hit
ATGAGGTCAAGTTAGTGGAAGAGGGGTGGATCGTCGGACTGTAGAACTCT21550.11452324848515769Illumina DpnII expression Sequencing Primer (100% over 22bp)
ATCATCAGAGGACAATTTCACATTCCTCACTAACAGTCAGATCGTCGGAC21310.11324781555539258No Hit
AGCCGACAGTACCCTAAGCGATCGTCGGACTGTAGAACTCTGAACGTGTA20790.11048437754090153Illumina DpnII expression Sequencing Primer (96% over 30bp)
ACATATGAGTTCAAACATACATGTAAGATGGATCGTCGGACTGTAGAACT19770.10506378758939987No Hit
AGAACCGGTGTTCTCCCTGTGGTATGGTCGTAGGCGATCGTCGGACTGTA19430.10325692427223265No Hit
AAGCCTACAACACGGGGTATTCCCAGGCGGTCACCGATCGTCGGACTGTA19310.1026192078073501No Hit
AAGGATGATAACCCTCGACCGTCCGGACCTCGATCGTCGGACTGTAGAAC19190.10198149134246755No Hit
AGTAGTAGATAACCCTCCCCAAGTCCGGACCTCGATCGTCGGACTGTAGA19190.10198149134246755No Hit
AGCCGACAGTACCCTAAGCACTTCCCAACTAGTCGATCGTCGGACTGTAG19180.10192834830372735No Hit
CATCCAAGTACTAACCCCGCCCGACAGGGCTTAACTTCGGTGATCAGACG19110.10155634703254586No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)