FastQCFastQC Report
Wed 30 Apr 2025
1H3_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1H3_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1881714
Total Bases282.2 Mbp
Sequences flagged as poor quality0
Sequence length150
%GC64

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTGCTTAAGCGTGAGTAGTTGTCTGCTAGATCGGAAGAGCACACGTCT1197426.363453744830511Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGGACTAGTTGGGAAGTGCTTAGGGTACTGTCGGCTAGATCGGAAGAGC702673.734201903158503No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTAGTGTG600643.1919834788921166TruSeq Adapter, Index 10 (97% over 46bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTAGTATG449472.3886201622563257TruSeq Adapter, Index 10 (97% over 49bp)
TGAAAATCTTTGCTCTGAAGTGGAAAGATCGGAAGAGCACACGTCTGAAC339131.8022398727968225Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GTGTGCTTAAGCGTGAGTAGTTGTCTGCTGTGTTTAGATCGGAAGAGCAC328231.744313960569991No Hit
GTGTGCTTAAGCGTGAGTAGTTGTCTAGATCGGAAGAGCACACGTCTGAA311491.6553525137188754Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTAGGGGG163200.867294392240266TruSeq Adapter, Index 10 (97% over 45bp)
GTTGCTACGATCTTCTGAGATTAAGCCTTTGTTCTAAGATTTGTAGATCG151860.8070301863088652No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTAGTGGG150180.7981021558005095TruSeq Adapter, Index 10 (97% over 46bp)
GTGTGCTTAAGCGTGAGTAGTTGTCTGCCAGATCGGAAGAGCACACGTCT129690.6892120694218143Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTGGGGGG128990.6854920567099995TruSeq Adapter, Index 10 (97% over 45bp)
TTGCTACGATCTTCTGAGATTAAGCCTTTGTTCTAAGATTTGTAGATCGG122920.6532342321946906No Hit
GTGTGCTTAAGCGTGAGTAGTTGTCTGCTGTGTTTCTTAAGATCGGAAGA120890.6424461953304275No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTGGTGTG116400.6185849709360721TruSeq Adapter, Index 10 (97% over 46bp)
GCACTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT101650.5401989887942589No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTAGGGTG80920.43003346948579857TruSeq Adapter, Index 10 (97% over 45bp)
AACTTTTGACGGTGGATCTCTTGGCTCACGTATCGATGAAGAACGCAGCC74370.39522477911095943No Hit
GGGGACTAGTTGGGAAGTGCTTAGGGTACTGTCGGCCAGATCGGAAGAGC71850.38183273334842593No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTAGTAGG67040.3562709317143838TruSeq Adapter, Index 10 (97% over 47bp)
GTATAATTGTGAGGTGTGTCTTTTCTTAAAGCAGATCGGAAGAGCACACG62260.3308685591965623No Hit
TGACTGTTAGTGAGGAATGTGAAATTGTCCTCTGATGATAAGATCGGAAG57140.30365932336157353No Hit
GCTACGATCTTCTGAGATTAAGCCTTTGTTCTAAGATTTGTAGATCGGAA55100.2928181434585702No Hit
ATGTACAATTCTAGACTCTTTGGTGGTAGATCGGAAGAGCACACGTCTGA50160.26656548232090527Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTGTGCTTAAGCGTGAGTAGTTGTCTGCAGATCGGAAGAGCACACGTCTG50000.26571519370106195Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTATTGTAAGTGCTGAGAGTAGATCGGAAGAGCACACGTCTGAACTCCAG43810.23281965272087043Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GACTGTTAGTGAGGAATGTGAAATTGTCCTCTGATGATAAGATCGGAAGA42350.22506076906479944No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTGGTGGG41600.2210750411592835TruSeq Adapter, Index 10 (97% over 46bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTGGTATG41000.21788645883487073TruSeq Adapter, Index 10 (97% over 49bp)
GTGACCTCTGGCAGCATGAATGTGCACAACTCTGCCAGATCGGAAGAGCA40750.21655788286636546No Hit
TGCTACGATCTTCTGAGATTAAGCCTTTGTTCTAAGATTTGTAGATCGGA40540.215441879052821No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTAGTATGCCGTC39090.2077361384354902TruSeq Adapter, Index 10 (98% over 50bp)
TTGTTTAAATATTCTGTTGATGAACTCAGCAGATCGGAAGAGCACACGTC39040.20747042324178916No Hit
GAGGTCCGGACTTGGGGAGGGTTATCTACTAGATCGGAAGAGCACACGTC33570.17840118105089295No Hit
TTGACCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTAT32120.17069544043356216TruSeq Adapter, Index 10 (100% over 41bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTGGGGTG31920.16963257965875791TruSeq Adapter, Index 10 (97% over 45bp)
GTTCTTCTTCGCAAAGACTGTGTGTGCTCTTAACTGAGTAGATCGGAAGA31850.1692605783875764No Hit
GTGTGCTCAGGATCTGTGACGTTTACTTTAGATCGGAAGAGCACACGTCT30140.1601731187630001Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTAGTGTGCCGTC29380.156134247818744TruSeq Adapter, Index 10 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTAGGATG29330.1558685326250429TruSeq Adapter, Index 10 (97% over 45bp)
CTTGACTGTGTGGTGCGGTATTTGGGACATTTACCTTAGATCGGAAGAGC28920.1536896680366942No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTAGGAGG28490.15140451737086508TruSeq Adapter, Index 10 (97% over 45bp)
TGGGAATACCCCGTGTTGTAGGCTTAGATCGGAAGAGCACACGTCTGAAC27520.14624964261306447Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TGTTGCTACGATCTTCTGAGATTAAGCCTTTGTTCTAAGATTTGTAGATC26530.14098848177778345No Hit
GTGTGCTTAAGCGTGAGTAGTTGTCCAGATCGGAAGAGCACACGTCTGAA25910.13769361337589028Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTAGTGTGTCGTC24990.13280445381179073TruSeq Adapter, Index 10 (97% over 42bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACAGATCGGAAGAGCACACG24360.12945644237115736No Hit
GAGATTGCTGTAGGCTGCTATGAGCCTTGGGCAGATCGGAAGAGCACACG23280.12371699418721444No Hit
CACCCCTCTTCCACTAACTTGACCTCATAGATCGGAAGAGCACACGTCTG22100.11744611561586935Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGACTGTTAGTGAGGAATGTGAAATTGTCCTCTGATGATAGATCGGAAGA21050.11186609654814707No Hit
GCTTAGGGTACTGTCGGCTAGATCGGAAGAGCACACGTCTGAACTCCAGT20370.10825236991381262Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CATCTTACATGTATGTTTGAACTCATATGTAGATCGGAAGAGCACACGTC19830.10538264582184115No Hit
GCCTACGACCATACCACAGGGAGAACACCGGTTCTAGATCGGAAGAGCAC19560.10394778377585542No Hit
GGTGACCGCCTGGGAATACCCCGTGTTGTAGGCTTAGATCGGAAGAGCAC19390.10304435211727181No Hit
GAGGTCCGGACTTGGGGAGGGTTATCTACTACTAGATCGGAAGAGCACAC19280.10245977869112947No Hit
GAGGTCCGGACGGTCGAGGGTTATCATCCTTAGATCGGAAGAGCACACGT19200.10203463438120776No Hit
GACTAGTTGGGAAGTGCTTAGGGTACTGTCGGCTAGATCGGAAGAGCACA19180.10192834830372735No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)