FastQCFastQC Report
Wed 30 Apr 2025
1H2_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1H2_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1171673
Total Bases175.7 Mbp
Sequences flagged as poor quality0
Sequence length150
%GC68

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA17876015.256816534988857Illumina RNA PCR Primer (100% over 45bp)
TTCCACTTCAGAGCAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA613765.238321613624279Illumina DpnII expression Sequencing Primer (100% over 25bp)
TCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATCATT404613.4532672511869777Illumina RNA PCR Primer (100% over 50bp)
ACAAATCTTAGAACAAAGGCTTAATCTCAGAAGATCGTAGCAAGATCGTC339282.895688472807686No Hit
ACAAATCTTAGAACAAAGGCTTAATCTCAGAAGATCGTAGCAACGATCGT300282.562831097072306No Hit
AGTAGATAACCCTCCCCAAGTCCGGACCTCGATCGTCGGACTGTAGAACT66520.5677351957414739No Hit
AATGAGGTCAATGAAGGCGGGGAGGGGCGTACCGAAGGATCGTCGGACTG64700.5522018515404895No Hit
ACAAATCTTAGAACAAAGGCTTAATCTCAGAAGATCGTAGCGATCGTCGG55750.47581535121147284No Hit
CTGGCTGCGTTCTTCATCGATACGTGAGCCAAGAGATCCACCGTCAAAAG43950.3751046580402552No Hit
ACAAATCTTAGAACAAAGGCTTAATCTCAGAAGATCGTAGCAGATCGTCG41660.35555995572143423No Hit
GCAAATCTTAGAACAAAGGCTTAATCTCAGAAGATCGTAGCAAGATCGTC38350.3273097528064571No Hit
GCAAATCTTAGAACAAAGGCTTAATCTCAGAAGATCGTAGCAACGATCGT33200.28335550960037487No Hit
ATGAGGTCAAGTTAGTGGAAGAGGGGTGGATCGTCGGACTGTAGAACTCT32040.2734551363733738Illumina DpnII expression Sequencing Primer (100% over 22bp)
ACAAATCTTAGAACAAAGGCTTAATCTCAGAAGATCGTAGCAACAGATCG29250.24964303180153505No Hit
GGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAAC29070.2481067669904487No Hit
AATGAGGTCAAGTTAGTGGAAGAGGGGTGGATCGTCGGACTGTAGAACTC28020.23914522225911153Illumina DpnII expression Sequencing Primer (100% over 21bp)
TTTTTTTTTCCTTCACGAGTAAACATTCTAGATCGTCGGACTGTAGAACT26560.2266844076802999No Hit
GAGGTCAAGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGG25640.21883238753474732Illumina RNA PCR Primer (100% over 37bp)
TTTTTTTGGTCTTTCACCTACAGCATTAATAGATCGTCGGACTGTAGAAC25560.21814960317426446No Hit
GCTTTAAGAAAAGACACACCTCACAATTATACGATCGTCGGACTGTAGAA24410.2083345779923238No Hit
ACACATCCACTAGCGGTAGGTTGCCAGGATCGTCGGACTGTAGAACTCTG23720.20244556288315937Illumina DpnII expression Sequencing Primer (100% over 23bp)
AGAATCGTGGATAACCCTCCTCCGTCCGGACCTCGATCGTCGGACTGTAG21770.1858026940963904No Hit
TAGTAGATAACCCTCCCCAAGTCCGGACCTCGATCGTCGGACTGTAGAAC21660.18486386560072648No Hit
AGTAGTAGATAACCCTCCCCAAGTCCGGACCTCGATCGTCGGACTGTAGA21540.18383968906000223No Hit
TGTCTTTCACCTACAGCATTAATAGATCGTCGGACTGTAGAACTCTGAAC21300.18179133597855376Illumina DpnII expression Sequencing Primer (100% over 26bp)
AAAGAGGTCAAGTATTTGGAGGAGGGGTGTCTATAGAATCGATCGTCGGA20870.17812137004095852No Hit
AGTAGATAACCCTCCCCAAATCCGGACCTCGATCGTCGGACTGTAGAACT20730.17692649741011357No Hit
TCCATGAATTAATGAGCCGATCGATCGTCGGACTGTAGAACTCTGAACGT19200.16386824651587945Illumina DpnII expression Sequencing Primer (96% over 28bp)
AATGAGGTCAAGTTAGTGGAAGAGGGGTGATTCATGAGTTTCGATCGTCG19180.16369755042575873No Hit
TCCACTTCAGAGCAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAAC18550.15832062358695642Illumina DpnII expression Sequencing Primer (100% over 26bp)
AACTGATTTGTAAGTAGTAGATAACCCTCCCCAAGTCCGGACCTCGATCG16820.1435554117915152No Hit
ACCCGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCTGAAC15990.13647152405150584Illumina DpnII expression Sequencing Primer (100% over 26bp)
AAGTAGTAGATAACCCTCCCCAAGTCCGGACCTCGATCGTCGGACTGTAG15970.13630082796138512No Hit
AAAGTAAACGTCACAGATCCTGAGCACACGATCGTCGGACTGTAGAACTC15950.13613013187126444Illumina DpnII expression Sequencing Primer (100% over 21bp)
AAAGAGGTCAAGTATTTGGAGGAGGGGTGTCTATAGAATCGGATCGTCGG15760.13450851901511768No Hit
ATCATCAGAGGACAATTTCACATTCCTCACTAACAGTCAGATCGTCGGAC15640.13348434247439345No Hit
TGTAAGTAGTAGATAACCCTCCCCAAGTCCGGACCTCGATCGTCGGACTG14600.12460814578811666No Hit
AATTCAGAAAAACTGAACAGTCATCAGTGCTGATCGTCGGACTGTAGAAC14520.12392536142763382No Hit
TTTTCCTCCAATCCTCACGGCATAAACAAGATCGTCGGACTGTAGAACTC14200.1211942239857025Illumina DpnII expression Sequencing Primer (100% over 21bp)
AAGTTCTTGGAGCCAACTTATTATGGACTTGTAGATCGTCGGACTGTAGA13680.1167561256425641No Hit
TACTAATGAGGGATAACCCTCCCCTGAGTTCCGGACCTCGATCGTCGGAC13560.11573194910183984No Hit
ACAAGTTCGGATCTACGGGAGATCGTCGGACTGTAGAACTCTGAACGTGT12370.10557553173965774Illumina DpnII expression Sequencing Primer (96% over 30bp)
AAGCCTACAACACGGGGTATTCCCAGGCGGTCACCGATCGTCGGACTGTA11910.10164952166688147No Hit
TAAGTAGTAGATAACCCTCCCCAAGTCCGGACCTCGATCGTCGGACTGTA11850.10113743339651934No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)