FastQCFastQC Report
Wed 30 Apr 2025
1H2_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1H2_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1171673
Total Bases175.7 Mbp
Sequences flagged as poor quality0
Sequence length150
%GC65

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTAGTATG940208.024423196574471TruSeq Adapter, Index 6 (97% over 49bp)
TGAAAATCTTTGCTCTGAAGTGGAAAGATCGGAAGAGCACACGTCTGAAC595655.083756304019978Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTGCTACGATCTTCTGAGATTAAGCCTTTGTTCTAAGATTTGTAGATCGG340402.9052474538544457No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTAGTGTG332342.8364569295358004TruSeq Adapter, Index 6 (97% over 46bp)
GTTGCTACGATCTTCTGAGATTAAGCCTTTGTTCTAAGATTTGTAGATCG300322.5631724892525476No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTAGTATGCCGTC129791.1077322768383329TruSeq Adapter, Index 6 (98% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTAGTGGG70770.6040081148921244TruSeq Adapter, Index 6 (97% over 46bp)
AACTTTTGACGGTGGATCTCTTGGCTCACGTATCGATGAAGAACGCAGCC68500.584634108663424No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTAGTAGG66070.563894533713758TruSeq Adapter, Index 6 (97% over 47bp)
GAGGTCCGGACTTGGGGAGGGTTATCTACTAGATCGGAAGAGCACACGTC66010.5633824454433959No Hit
CTTCGGTACGCCCCTCCCCGCCTTCATTGACCTCATTAGATCGGAAGAGC64500.5504948906392825No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTAGGGGG58560.49979815187343224TruSeq Adapter, Index 6 (97% over 45bp)
GCTACGATCTTCTGAGATTAAGCCTTTGTTCTAAGATTTGTAGATCGGAA55960.4776076601577402No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTGGTATG50640.432202500185632TruSeq Adapter, Index 6 (97% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTGGTGTG49140.4194002934265789TruSeq Adapter, Index 6 (97% over 46bp)
TGCTACGATCTTCTGAGATTAAGCCTTTGTTCTAAGATTTGTAGATCGGA41770.35649878421709813No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTGGGGGG40000.3413921802414155TruSeq Adapter, Index 6 (97% over 45bp)
TTGCTACGATCTTCTGAGATTAAGCCTTTGTTCTAAGATTTGCAGATCGG38780.3309797187440523No Hit
GTTGCTACGATCTTCTGAGATTAAGCCTTTGTTCTAAGATTTGCAGATCG33300.2842089900509784No Hit
CACCCCTCTTCCACTAACTTGACCTCATAGATCGGAAGAGCACACGTCTG32070.27371118050855486Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTAGTGTGCCGTC31680.27038260675120107TruSeq Adapter, Index 6 (97% over 42bp)
GCACTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT29450.25134999270274216No Hit
TGTTGCTACGATCTTCTGAGATTAAGCCTTTGTTCTAAGATTTGTAGATC29250.24964303180153505No Hit
CACCCCTCTTCCACTAACTTGACCTCATTAGATCGGAAGAGCACACGTCT28590.2440100608275517Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TAGAATGTTTACTCGTGAAGGAAAAAAAAAAGATCGGAAGAGCACACGTC26320.22463605459885136No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTAGTATGTCGTC26060.2224170054272822TruSeq Adapter, Index 6 (97% over 45bp)
TATTAATGCTGTAGGTGAAAGACCAAAAAAAAGATCGGAAGAGCACACGT25500.21763751490390237No Hit
TTGACCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATAT25370.21652799031811779TruSeq Adapter, Index 6 (100% over 41bp)
GTATAATTGTGAGGTGTGTCTTTTCTTAAAGCAGATCGGAAGAGCACACG23880.20381113160412503No Hit
CTGGCAACCTACCGCTAGTGGATGTGTAGATCGGAAGAGCACACGTCTGA23550.20099464611713336Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GAGGTCCGGACGGAGGAGGGTTATCCACGATTCTAGATCGGAAGAGCACA21820.18622943432169214No Hit
GAGGTCCGGACTTGGGGAGGGTTATCTACTACTAGATCGGAAGAGCACAC21380.18247412033903657No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTAGGGTG21360.18230342424891585TruSeq Adapter, Index 6 (97% over 45bp)
GAGGTCCGGACTTGGGGAGGGTTATCTACTAAGATCGGAAGAGCACACGT21310.1818766840236141No Hit
TATTAATGCTGTAGGTGAAAGACAAGATCGGAAGAGCACACGTCTGAACT20970.17897485049156206Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GATTCTATAGACACCCCTCCTCCAAATACTTGACCTCTTTAGATCGGAAG20950.17880415440144135No Hit
GAGGTCCGGATTTGGGGAGGGTTATCTACTAGATCGGAAGAGCACACGTC20660.1763290610946911No Hit
GAAACTCATGAATCACCCCTCTTCCACTAACTTGACCTCATTAGATCGGA19340.1650631191467244No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTGGTGGG19270.16446568283130192TruSeq Adapter, Index 6 (97% over 46bp)
GATCGGCTCATTAATTCATGGAAGATCGGAAGAGCACACGTCTGAACTCC18260.15584553028020617Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGAAAATCTTTGCTCTGAAGTGGAAGATCGGAAGAGCACACGTCTGAACT17960.15328508892839557Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GAGGTCCGGACTTGGGGAGGGTTATCTACTACTTACAAATCAGTTAGATC16770.14312867156621342No Hit
CGATTCTATAGACACCCCTCCTCCAAATACTTGACCTCTTTAGATCGGAA16070.13715430841198867No Hit
GAGGTCCGGACTTGGGGAGGGTTATCTACTACTTAGATCGGAAGAGCACA15820.13502060728547982No Hit
GTGTGCTCAGGATCTGTGACGTTTACTTTAGATCGGAAGAGCACACGTCT15770.13459386706017806Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTCGGGCTGAGACTTGAAGCGGGTAGATCGGAAGAGCACACGTCTGAACT15630.13339899442933312Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TGACTGTTAGTGAGGAATGTGAAATTGTCCTCTGATGATAGATCGGAAGA15510.13237481788860886No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTAGTGTGTCGTC15130.1291315921763154TruSeq Adapter, Index 6 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTAGTATT15090.12879019999607397TruSeq Adapter, Index 6 (97% over 48bp)
AGCACTGATGACTGTTCAGTTTTTCTGAATTAGATCGGAAGAGCACACGT14640.12494953796835806No Hit
GAGGTCCGGACTTGGGGAGGGTTATCTACTACTTACAAGATCGGAAGAGC14440.12324257706715097No Hit
TTGTTTATGCCGTGAGGATTGGAGGAAAAAGATCGGAAGAGCACACGTCT13980.1193165669943747Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TACAAGTCCATAATAAGTTGGCTCCAAGAACTTAGATCGGAAGAGCACAC13980.1193165669943747No Hit
GAGGTCCGGAACTCAGGGGAGGGTTATCCCTCATTAGTAAGATCGGAAGA13410.11445172842593453No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTAGTATGCCGTT12600.10753853677604587TruSeq Adapter, Index 6 (97% over 49bp)
TCCCGTAGATCCGAACTTGTAGATCGGAAGAGCACACGTCTGAACTCCAG12120.10344183061314889Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GGTGACCGCCTGGGAATACCCCGTGTTGTAGGCTTAGATCGGAAGAGCAC11890.10147882557676076No Hit
GTTGTGGCGGTCCGGGGCTTGCGGATCTGAATGGACCGCGGTCAGATCGG11750.10028395294591579No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)