FastQCFastQC Report
Wed 30 Apr 2025
1H1_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1H1_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3188669
Total Bases478.3 Mbp
Sequences flagged as poor quality0
Sequence length150
%GC68

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA34379910.78189677260324Illumina RNA PCR Primer (100% over 45bp)
TTCCACTTCAGAGCAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA1472594.618196495152053Illumina DpnII expression Sequencing Primer (100% over 25bp)
TCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATCATT1401814.396223000882187Illumina RNA PCR Primer (100% over 50bp)
ACAAATCTTAGAACAAAGGCTTAATCTCAGAAGATCGTAGCAACGATCGT729222.2869103064632923No Hit
ACAAATCTTAGAACAAAGGCTTAATCTCAGAAGATCGTAGCAAGATCGTC687212.1551625458772925No Hit
AGCAGACAACTACTCACGCTTAAGCACACGATCGTCGGACTGTAGAACTC385611.2093133530008915Illumina DpnII expression Sequencing Primer (100% over 21bp)
ACAAATCTTAGAACAAAGGCTTAATCTCAGAAGATCGTAGCGATCGTCGG218920.6865560520706289No Hit
AGACAACTACTCACGCTTAAGCACACGATCGTCGGACTGTAGAACTCTGA181860.5703320100016652Illumina DpnII expression Sequencing Primer (100% over 24bp)
AAACACAGCAGACAACTACTCACGCTTAAGCACACGATCGTCGGACTGTA169690.5321656151830121No Hit
ACAAATCTTAGAACAAAGGCTTAATCTCAGAAGATCGTAGCAGATCGTCG123230.3864621884554339No Hit
GCAAATCTTAGAACAAAGGCTTAATCTCAGAAGATCGTAGCAACGATCGT112220.3519336751478438No Hit
GCAAATCTTAGAACAAAGGCTTAATCTCAGAAGATCGTAGCAAGATCGTC105680.33142355007685026No Hit
AGCCGACAGTACCCTAAGCACTTCCCAACTAGTCCCCGATCGTCGGACTG98760.30972170520050846No Hit
CTGGCTGCGTTCTTCATCGATACGTGAGCCAAGAGATCCACCGTCAAAAG96600.3029477189385289No Hit
ATGAGGTCAAGTTAGTGGAAGAGGGGTGGATCGTCGGACTGTAGAACTCT91100.28569914280848846Illumina DpnII expression Sequencing Primer (100% over 22bp)
ACAAATCTTAGAACAAAGGCTTAATCTCAGAAGATCGTAGCAACAGATCG89900.2819358171073887No Hit
GAGGTCAAGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGG87420.2741582773251159Illumina RNA PCR Primer (100% over 37bp)
GCTTTAAGAAAAGACACACCTCACAATTATACGATCGTCGGACTGTAGAA82950.26013988908851937No Hit
AATGAGGTCAAGTTAGTGGAAGAGGGGTGGATCGTCGGACTGTAGAACTC77120.2418563983906765Illumina DpnII expression Sequencing Primer (100% over 21bp)
GGCAGACAACTACTCACGCTTAAGCACACGATCGTCGGACTGTAGAACTC72900.22862203634180905Illumina DpnII expression Sequencing Primer (100% over 21bp)
TTTTTTTGGTCTTTCACCTACAGCATTAATAGATCGTCGGACTGTAGAAC65320.20485036232986237No Hit
TTTTTTTTTCCTTCACGAGTAAACATTCTAGATCGTCGGACTGTAGAACT62030.1945325776993473No Hit
AAAGTAAACGTCACAGATCCTGAGCACACGATCGTCGGACTGTAGAACTC59960.18804084086495024Illumina DpnII expression Sequencing Primer (100% over 21bp)
GGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAAC56260.17643725328655938No Hit
TGTCTTTCACCTACAGCATTAATAGATCGTCGGACTGTAGAACTCTGAAC50380.15799695735117064Illumina DpnII expression Sequencing Primer (100% over 26bp)
AGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAAC48490.15206971937193858No Hit
AAGCCTACAACACGGGGTATTCCCAGATCGTCGGACTGTAGAACTCTGAA48140.1509720827091178Illumina DpnII expression Sequencing Primer (100% over 25bp)
AGTAGATAACCCTCCCCAAGTCCGGACCTCGATCGTCGGACTGTAGAACT43860.13754955437519542No Hit
TCCACTTCAGAGCAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAAC40650.12748265812475362Illumina DpnII expression Sequencing Primer (100% over 26bp)
ACAAGTTCGGATCTACGGGAGATCGTCGGACTGTAGAACTCTGAACGTGT38860.12186903062061318Illumina DpnII expression Sequencing Primer (96% over 30bp)
CCTTTTAGGAGCGTCATATTTTACATTTTAGATCGTCGGACTGTAGAACT36300.11384060245826708No Hit
AATGCTTTCGCAGTAGTTCGTCTTTCGTAAATCGATCGTCGGACTGTAGA36180.11346426988815711No Hit
ACTCACTCACGCCACAGAAGATCGTCGGACTGTAGAACTCTGAACGTGTA35480.11126899656251558Illumina DpnII expression Sequencing Primer (96% over 30bp)
GCTGGCTGCGTTCTTCATCGATACGTGAGCCAAGAGATCCACCGTCAAAA34960.10963822209203904No Hit
GCAAATCTTAGAACAAAGGCTTAATCTCAGAAGATCGTAGCGATCGTCGG34520.10825833600163579No Hit
ACTTTAAGAAAAGACACACCTCACAATTATACGATCGTCGGACTGTAGAA34180.1071920603863242No Hit
AGTAAACGTCACAGATCCTGAGCACACGATCGTCGGACTGTAGAACTCTG33340.10455773239555438Illumina DpnII expression Sequencing Primer (100% over 23bp)
AGAATCGTGGATAACCCTCCTCCGTCCGGACCTCGATCGTCGGACTGTAG32490.1018920433572754No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)