FastQCFastQC Report
Wed 30 Apr 2025
1H1_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1H1_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3188669
Total Bases478.3 Mbp
Sequences flagged as poor quality0
Sequence length150
%GC65

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAAAATCTTTGCTCTGAAGTGGAAAGATCGGAAGAGCACACGTCTGAAC1431054.487922703798984Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GTTGCTACGATCTTCTGAGATTAAGCCTTTGTTCTAAGATTTGTAGATCG728502.2846523110426324No Hit
TTGCTACGATCTTCTGAGATTAAGCCTTTGTTCTAAGATTTGTAGATCGG689892.1635673066097487No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTAGTATG672922.1103476089866966TruSeq Adapter, Index 3 (97% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTAGTGTG472611.4821544663306228TruSeq Adapter, Index 3 (97% over 46bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTGGTGTG388201.2174358643057652TruSeq Adapter, Index 3 (97% over 46bp)
GTGTGCTTAAGCGTGAGTAGTTGTCTGCTAGATCGGAAGAGCACACGTCT385511.2089997425258Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTGGGGGG348071.0915839806514882TruSeq Adapter, Index 3 (97% over 45bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTGGGGTG283850.8901833335476339TruSeq Adapter, Index 3 (97% over 45bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTGGTATG266460.8356464719291967TruSeq Adapter, Index 3 (97% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTAGGGTG231180.7250046963168645TruSeq Adapter, Index 3 (97% over 45bp)
AACTTTTGACGGTGGATCTCTTGGCTCACGTATCGATGAAGAACGCAGCC230470.7227780619437139No Hit
GCTACGATCTTCTGAGATTAAGCCTTTGTTCTAAGATTTGTAGATCGGAA219550.6885317980637062No Hit
GTGTGCTTAAGCGTGAGTAGTTGTCTAGATCGGAAGAGCACACGTCTGAA179880.5641225225948507Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTAGGATG177610.5570035648102704TruSeq Adapter, Index 3 (97% over 45bp)
GTGTGCTTAAGCGTGAGTAGTTGTCTGCTGTGTTTAGATCGGAAGAGCAC170090.5334200570833786No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTAGGGGG138460.4342250638118914TruSeq Adapter, Index 3 (97% over 45bp)
GGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTAGTATGCCGTC133920.41998714824273076TruSeq Adapter, Index 3 (98% over 50bp)
TGCTACGATCTTCTGAGATTAAGCCTTTGTTCTAAGATTTGTAGATCGGA124460.3903195972990612No Hit
GTTGCTACGATCTTCTGAGATTAAGCCTTTGTTCTAAGATTTGCAGATCG111570.34989520705974814No Hit
TTGCTACGATCTTCTGAGATTAAGCCTTTGTTCTAAGATTTGCAGATCGG105490.3308276901741761No Hit
GGGGACTAGTTGGGAAGTGCTTAGGGTACTGTCGGCTAGATCGGAAGAGC98540.3090317621553068No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTGGGATG92650.29056010517240893TruSeq Adapter, Index 3 (97% over 45bp)
CACCCCTCTTCCACTAACTTGACCTCATAGATCGGAAGAGCACACGTCTG92240.2892743022245332Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGTTGCTACGATCTTCTGAGATTAAGCCTTTGTTCTAAGATTTGTAGATC89510.2807127362545313No Hit
TTGACCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCAT86070.2699245359113787TruSeq Adapter, Index 3 (100% over 41bp)
GTATAATTGTGAGGTGTGTCTTTTCTTAAAGCAGATCGGAAGAGCACACG82820.25973219547090026No Hit
CACCCCTCTTCCACTAACTTGACCTCATTAGATCGGAAGAGCACACGTCT79300.24869310674767434Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTGTGCTTAAGCGTGAGTAGTTGTCTGCCAGATCGGAAGAGCACACGTCT74250.23285577775554628Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TATTAATGCTGTAGGTGAAAGACCAAAAAAAAGATCGGAAGAGCACACGT65530.20550894432755487No Hit
TAGAATGTTTACTCGTGAAGGAAAAAAAAAAGATCGGAAGAGCACACGTC62040.19456393874685646No Hit
GTGTGCTCAGGATCTGTGACGTTTACTTTAGATCGGAAGAGCACACGTCT60180.18873078391015186Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTAGTGTGCCGTC59330.18606509487187287TruSeq Adapter, Index 3 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTAGGAGG57610.18067099470029657TruSeq Adapter, Index 3 (97% over 45bp)
GCACTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT57070.1789774981348017No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTGGTATGCCGTC54640.17135676359007473TruSeq Adapter, Index 3 (98% over 50bp)
TATTAATGCTGTAGGTGAAAGACAAGATCGGAAGAGCACACGTCTGAACT49620.15561351774047416Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GCACTGGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGT49210.1543277147925984No Hit
TGGGAATACCCCGTGTTGTAGGCTTAGATCGGAAGAGCACACGTCTGAAC47400.14865136519343966Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTGGTGGG46660.14633064767776147TruSeq Adapter, Index 3 (97% over 46bp)
GAGGTCCGGACTTGGGGAGGGTTATCTACTAGATCGGAAGAGCACACGTC44010.13801997008783287No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTGGTGTGTCGTC43550.13657736190241132TruSeq Adapter, Index 3 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTAGTGGG40870.12817260116995524TruSeq Adapter, Index 3 (97% over 46bp)
GGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTGGTGTGCCGTC39270.12315483356848893TruSeq Adapter, Index 3 (97% over 42bp)
TGAAAATCTTTGCTCTGAAGTGGAAGATCGGAAGAGCACACGTCTGAACT38870.12190039166812235Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTAGTATGTCGTC38690.12133589281295737TruSeq Adapter, Index 3 (97% over 45bp)
TCCCGTAGATCCGAACTTGTAGATCGGAAGAGCACACGTCTGAACTCCAG38330.12020689510262746Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTAGTGTGTCGTC37440.11741576187431182TruSeq Adapter, Index 3 (97% over 42bp)
GATTTACGAAAGACGAACTACTGCGAAAGCATTAGATCGGAAGAGCACAC36360.11402876874332206No Hit
TAAAATGTAAAATATGACGCTCCTAAAAGGAGATCGGAAGAGCACACGTC36290.1138092414107579No Hit
TTCTGTGGCGTGAGTGAGTAGATCGGAAGAGCACACGTCTGAACTCCAGT34890.10941869475947488Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTGGGAGG34890.10941869475947488TruSeq Adapter, Index 3 (97% over 45bp)
GCTACGATCTTCTGAGATTAAGCCTTTGTTCTAAGATTTGCAGATCGGAA34720.10888555695181908No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTAGTAGG34070.10684708886372339TruSeq Adapter, Index 3 (97% over 47bp)
GTATAATTGTGAGGTGTGTCTTTTCTTAAAGTAGATCGGAAGAGCACACG33630.10546720277332015No Hit
GTGTGCTCAGGATCTGTGACGTTTACTAGATCGGAAGAGCACACGTCTGA33090.10377370620782526Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GAGGTCCGGACGGAGGAGGGTTATCCACGATTCTAGATCGGAAGAGCACA32580.10217429278485787No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTGGTGTGTCGTT32360.10148434973965625TruSeq Adapter, Index 3 (97% over 42bp)
GTGTGCTTAAGCGTGAGTAGTTGTCCAGATCGGAAGAGCACACGTCTGAA32330.10139026659712878Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)