FastQCFastQC Report
Wed 30 Apr 2025
1G3_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1G3_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1627005
Total Bases244 Mbp
Sequences flagged as poor quality0
Sequence length150
%GC69

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA30322718.637127728556457Illumina RNA PCR Primer (100% over 45bp)
TTCCACTTCAGAGCAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA1277127.849514906223398Illumina DpnII expression Sequencing Primer (100% over 25bp)
TCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATCATT423882.605277795704377Illumina RNA PCR Primer (100% over 50bp)
ACAAATCTTAGAACAAAGGCTTAATCTCAGAAGATCGTAGCAAGATCGTC340052.0900366009938507No Hit
ACAAATCTTAGAACAAAGGCTTAATCTCAGAAGATCGTAGCAACGATCGT265771.633492214221837No Hit
GCTGAGTTCATCAACAGAATATTTAAACAAGATCGTCGGACTGTAGAACT89170.5480622370552026No Hit
GGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAAC86380.530914164369501No Hit
GCTTTAAGAAAAGACACACCTCACAATTATACGATCGTCGGACTGTAGAA77640.47719582914619196No Hit
ACAAATCTTAGAACAAAGGCTTAATCTCAGAAGATCGTAGCGATCGTCGG74590.458449728181536No Hit
CTGGCTGCGTTCTTCATCGATACGTGAGCCAAGAGATCCACCGTCAAAAG63960.39311495662275164No Hit
GAGGTCAAGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGG61490.377933687972686Illumina RNA PCR Primer (100% over 37bp)
ACCCGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCTGAAC55690.34228536482678296Illumina DpnII expression Sequencing Primer (100% over 26bp)
ACAAATCTTAGAACAAAGGCTTAATCTCAGAAGATCGTAGCAGATCGTCG47380.2912099225263598No Hit
ACCCGCTTCAAGTCTCAGCCCGAGAGATCGTCGGACTGTAGAACTCTGAA43610.2680385124815228Illumina DpnII expression Sequencing Primer (100% over 25bp)
TGTCTTTCACCTACAGCATTAATAGATCGTCGGACTGTAGAACTCTGAAC43010.2643507549147052Illumina DpnII expression Sequencing Primer (100% over 26bp)
AGAGCAGGGAAATTCGTGATGGATCGTCGGACTGTAGAACTCTGAACGTG37250.22894828227325667Illumina DpnII expression Sequencing Primer (96% over 29bp)
TCCACTTCAGAGCAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAAC35240.21659429442441785Illumina DpnII expression Sequencing Primer (100% over 26bp)
TTTTTTTTTCCTTCACGAGTAAACATTCTAGATCGTCGGACTGTAGAACT33790.20768221363794212No Hit
CCGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCTGAACGT32400.1991389086081481Illumina DpnII expression Sequencing Primer (96% over 28bp)
TTTTTTTGGTCTTTCACCTACAGCATTAATAGATCGTCGGACTGTAGAAC29210.17953233087790144No Hit
GGCAGAGTTGTGCACATTCATGCTGCCAGAGGTCACGATCGTCGGACTGT27830.17105048847422102No Hit
AGTAGATAACCCTCCCCAAGTCCGGACCTCGATCGTCGGACTGTAGAACT25560.15709847234642796No Hit
ACAAATCTTAGAACAAAGGCTTAATCTCAGAAGATCGTAGCAACAGATCG21870.13441876331049996No Hit
GCAAATCTTAGAACAAAGGCTTAATCTCAGAAGATCGTAGCAAGATCGTC21300.13091539362202329No Hit
AAGCCTACAACACGGGGTATTCCCAGGCGGTCACCGATCGTCGGACTGTA19210.11806970476427546No Hit
ACCCGCTTCAAGTCTCGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT18360.11284538154461726Illumina DpnII expression Sequencing Primer (96% over 30bp)
ATGAGGTCAAGTTAGTGGAAGAGGGGTGGATCGTCGGACTGTAGAACTCT18000.11063272700452673Illumina DpnII expression Sequencing Primer (100% over 22bp)
GACCGCGGTCCATTCAGATCCGCAAGCCCCGGACCGCCACAACGATCGTC17620.10829714721220893No Hit
CCAAGACCACAAAGGTACCAGACATCGGAGATCGTCGGACTGTAGAACTC17450.1072522825682773Illumina DpnII expression Sequencing Primer (100% over 21bp)
AAATTCACTCAACCCAGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT17300.10633034317657292Illumina DpnII expression Sequencing Primer (96% over 30bp)
ACCCGCTTCAAGTCTCAGCCCGGATCGTCGGACTGTAGAACTCTGAACGT17060.10485524014984587Illumina DpnII expression Sequencing Primer (96% over 28bp)
GCAAATCTTAGAACAAAGGCTTAATCTCAGAAGATCGTAGCAACGATCGT16840.10350306237534611No Hit
TCCATGAATTAATGAGCCGATCGATCGTCGGACTGTAGAACTCTGAACGT16700.10264258560975534Illumina DpnII expression Sequencing Primer (96% over 28bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)