FastQCFastQC Report
Wed 30 Apr 2025
1G3_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1G3_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1627005
Total Bases244 Mbp
Sequences flagged as poor quality0
Sequence length150
%GC67

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTGGGGGG18177411.172307399178244TruSeq Adapter, Index 9 (97% over 45bp)
TGAAAATCTTTGCTCTGAAGTGGAAAGATCGGAAGAGCACACGTCTGAAC1262517.75971800947139Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTGGGGTG805124.948478953660253TruSeq Adapter, Index 9 (97% over 45bp)
TTGCTACGATCTTCTGAGATTAAGCCTTTGTTCTAAGATTTGTAGATCGG340872.095076536335168No Hit
GTTGCTACGATCTTCTGAGATTAAGCCTTTGTTCTAAGATTTGTAGATCG265871.6341068404829733No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTAGGGGG91310.5612152390435187TruSeq Adapter, Index 9 (97% over 45bp)
AACTTTTGACGGTGGATCTCTTGGCTCACGTATCGATGAAGAACGCAGCC90470.556052378449974No Hit
TTGTTTAAATATTCTGTTGATGAACTCAGCAGATCGGAAGAGCACACGTC88340.5429608390877717No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT87980.5407481845476811No Hit
GTATAATTGTGAGGTGTGTCTTTTCTTAAAGCAGATCGGAAGAGCACACG77250.4747987867277605No Hit
GCTACGATCTTCTGAGATTAAGCCTTTGTTCTAAGATTTGTAGATCGGAA74500.45789656454651334No Hit
TTGACCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGAT61100.3755366455542546TruSeq Adapter, Index 9 (100% over 41bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTAGGGTG59880.3680382051683922TruSeq Adapter, Index 9 (97% over 45bp)
CTCGGGCTGAGACTTGAAGCGGGTAGATCGGAAGAGCACACGTCTGAACT55350.3401956355389197Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TGCTACGATCTTCTGAGATTAAGCCTTTGTTCTAAGATTTGTAGATCGGA47320.29084114676967804No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTGGGAGG47200.2901035952563145TruSeq Adapter, Index 9 (97% over 45bp)
TCTCGGGCTGAGACTTGAAGCGGGTAGATCGGAAGAGCACACGTCTGAAC43890.2697594660127043Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TATTAATGCTGTAGGTGAAAGACAAGATCGGAAGAGCACACGTCTGAACT42750.26275272663575094Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTGGGGGGGGGGG42590.26176932461793295TruSeq Adapter, Index 9 (97% over 41bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTGGGATG38000.23355797923177865TruSeq Adapter, Index 9 (97% over 45bp)
CATCACGAATTTCCCTGCTCTAGATCGGAAGAGCACACGTCTGAACTCCA37680.2315911751961426Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGAAAATCTTTGCTCTGAAGTGGAAGATCGGAAGAGCACACGTCTGAACT34940.21475041564100908Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TAGAATGTTTACTCGTGAAGGAAAAAAAAAAGATCGGAAGAGCACACGTC33940.2086041530296465No Hit
CTCGGGCTGAGACTTGAAGCGGAGATCGGAAGAGCACACGTCTGAACTCC31670.1946521369018534Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TATTAATGCTGTAGGTGAAAGACCAAAAAAAAGATCGGAAGAGCACACGT29430.1808845086524012No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTGGGGTT29050.17854892886008342TruSeq Adapter, Index 9 (97% over 45bp)
GTGACCTCTGGCAGCATGAATGTGCACAACTCTGCCAGATCGGAAGAGCA27940.17172657736147093No Hit
GAGGTCCGGACTTGGGGAGGGTTATCTACTAGATCGGAAGAGCACACGTC25210.15494728043245104No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTGGGGGGGCGTT24150.1484322420644067TruSeq Adapter, Index 9 (97% over 41bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTGGTGTG23660.14542057338483902TruSeq Adapter, Index 9 (97% over 46bp)
GGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTGGGGTGGCGTT22380.1375533572422949TruSeq Adapter, Index 9 (97% over 41bp)
TGTTGCTACGATCTTCTGAGATTAAGCCTTTGTTCTAAGATTTGTAGATC21780.13386559967547734No Hit
TTGCTACGATCTTCTGAGATTAAGCCTTTGTTCTAAGATTTGCAGATCGG21630.13294366028377294No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTGGGGGT21120.129809066351978TruSeq Adapter, Index 9 (97% over 45bp)
GGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTGGGGTGTCGTT20550.12630569666350133TruSeq Adapter, Index 9 (97% over 41bp)
GGTGACCGCCTGGGAATACCCCGTGTTGTAGGCTTAGATCGGAAGAGCAC19380.11911456940820711No Hit
GAGACTTGAAGCGGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC18290.11241514316182187Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CACCCCTCTTCCACTAACTTGACCTCATAGATCGGAAGAGCACACGTCTG18170.11167759164845838Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTGGGGGGGCGGT17960.11038687650007221TruSeq Adapter, Index 9 (97% over 41bp)
GTTGTGGCGGTCCGGGGCTTGCGGATCTGAATGGACCGCGGTCAGATCGG17600.10817422195998168No Hit
TCCGATGTCTGGTACCTTTGTGGTCTTGGAGATCGGAAGAGCACACGTCT17160.10546986641098213Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGGGCTGAGACTTGAAGCGGGTAGATCGGAAGAGCACACGTCTGAACTCC16960.10424061388870963Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GTTGCTACGATCTTCTGAGATTAAGCCTTTGTTCTAAGATTTGCAGATCG16900.10387183813202787No Hit
TGGGTTGAGTGAATTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC16820.10338013712311886Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GATCGGCTCATTAATTCATGGAAGATCGGAAGAGCACACGTCTGAACTCC16480.10129040783525559Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)