FastQCFastQC Report
Wed 30 Apr 2025
1G1_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1G1_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43795569
Total Bases6.5 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC68

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATCATT25958085.927101894714508Illumina RNA PCR Primer (100% over 50bp)
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA22860335.2197814806333485Illumina RNA PCR Primer (100% over 45bp)
TTCCACTTCAGAGCAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA16016283.6570548952109747Illumina DpnII expression Sequencing Primer (100% over 25bp)
GCTGAGTTCATCAACAGAATATTTAAACAAGATCGTCGGACTGTAGAACT5200681.1874899947069988No Hit
GGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAAC3662320.8362307154863087No Hit
AGTAGATAACCCTCCCCAAGTCCGGACCTCGATCGTCGGACTGTAGAACT3330920.7605609599455141No Hit
AGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAAC2945440.6725429232349966No Hit
AAACTAACCAGCCTAGACCTGAGATCGTCGGACTGTAGAACTCTGAACGT2712000.6192407272982342Illumina DpnII expression Sequencing Primer (96% over 28bp)
ACTGAGTTCATCAACAGAATATTTAAACAAGATCGTCGGACTGTAGAACT2501000.571062337379382No Hit
AAGAATGGCGCAAGAATCCAAAGTGGTCAGATCGTCGGACTGTAGAACTC1791990.4091715305719627Illumina DpnII expression Sequencing Primer (100% over 21bp)
AAAGAGGTCAAGTATTTGGAGGAGGGGTGTCTATAGAATCGATCGTCGGA1681470.38393610093295055No Hit
AAGGATGATAACCCTCGACCGTCCGGACCTCGATCGTCGGACTGTAGAAC1487610.3396713489439993No Hit
TCCGACACTCAGACAGACATGCTCCTGGGAGAACCCAAGAGCGCCATTTG1349110.3080471451347053No Hit
AATGAGGTCAATGAAGGCGGGGAGGGGCGTACCGAAGGATCGTCGGACTG1324220.3023639217930928No Hit
ACAAGTTCGGATCTACGGGAGATCGTCGGACTGTAGAACTCTGAACGTGT1306530.2983247003823606Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGAATCGTGGATAACCCTCCTCCGTCCGGACCTCGATCGTCGGACTGTAG1217920.2780920599524578No Hit
ACAAATCTTAGAACAAAGGCTTAATCTCAGAAGATCGTAGCAACGATCGT1144610.26135292362567547No Hit
AAGCCTACAACACGGGGTATTCCCAGGCGGTCACCGATCGTCGGACTGTA969340.22133289328881647No Hit
ACAAATCTTAGAACAAAGGCTTAATCTCAGAAGATCGTAGCAAGATCGTC967940.22101322624670092No Hit
AAATGAGGTCAATGAAGGCGGGGAGGGGCGTACCGAAGGATCGTCGGACT964450.220216341977427No Hit
AATTTGCTCAAAAACCATTAGATCTAATCAGATCGTCGGACTGTAGAACT947800.21641458751226636No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG920110.2100920300864227No Hit
CGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATCATTA880900.20113906957117053Illumina RNA PCR Primer (100% over 49bp)
GTGGATAACCCTCCTCCGTCCGGACCTCGATCGTCGGACTGTAGAACTCT814590.1859982684549663Illumina DpnII expression Sequencing Primer (100% over 22bp)
GAGGTCAAGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGG794200.18134254632015398Illumina RNA PCR Primer (100% over 37bp)
AGTAGTAGATAACCCTCCCCAAGTCCGGACCTCGATCGTCGGACTGTAGA776050.17719829145272664No Hit
GGCGAGAATTCTACCACTGAACCACAGTGCGATCGTCGGACTGTAGAACT741570.16932534887262224No Hit
AGTAGATAACCCTCCCCAAATCCGGACCTCGATCGTCGGACTGTAGAACT660430.15079836044600767No Hit
CCTTTTAGGAGCGTCATATTTTACATTTTAGATCGTCGGACTGTAGAACT658600.15038050995524227No Hit
AAAGAGGTCAAGTATTTGGAGGAGGGGTGTCTATAGAATCGGATCGTCGG655820.1497457425430413No Hit
AAAGAGGTCAAGTATTTGGAGGAGGGGTGTCTATAGAATCGTGGGATCGT609730.13922184684939246No Hit
AAAATGAGGTCAATGAAGGCGGGGAGGGGCGTACCGAAGGATCGTCGGAC605900.13834732915560477No Hit
AGCGAGAATTCTACCACTGAACCACAGTGCGATCGTCGGACTGTAGAACT599730.13693851083428096No Hit
GGTAGATAACCCTCCCCAAGTCCGGACCTCGATCGTCGGACTGTAGAACT597550.1364407435829867No Hit
ACAAAGTTAGAAGTGTTGAAAGAGCAAAGATCGTCGGACTGTAGAACTCT565100.12903131821394992Illumina DpnII expression Sequencing Primer (100% over 22bp)
AATGGAAGCCATACAGGTTGTCACGTTGAAGATCGTCGGACTGTAGAACT558940.12762478322864124No Hit
AAACTAACCAGCCTAGACTTGAGATCGTCGGACTGTAGAACTCTGAACGT547770.1250742968997617Illumina DpnII expression Sequencing Primer (96% over 28bp)
GAATCGTGGATAACCCTCCTCCGTCCGGACCTCGATCGTCGGACTGTAGA530010.1210190921369237No Hit
AATCGTGGATAACCCTCCTCCGTCCGGACCTCGATCGTCGGACTGTAGAA494000.11279679914650727No Hit
AGCCGACAGTACCCTAAGCACTTCCCAACTAGTCCCCGATCGTCGGACTG482020.11006136260040371No Hit
AGGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAA456940.10433475587450411No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)