FastQCFastQC Report
Wed 30 Apr 2025
1G1_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1G1_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43795569
Total Bases6.5 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC64

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTAGTATG17031573.8888797174892282TruSeq Adapter, Index 2 (97% over 49bp)
GGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTAGTATGCCGTC16421283.7495300038229895TruSeq Adapter, Index 2 (98% over 50bp)
TGAAAATCTTTGCTCTGAAGTGGAAAGATCGGAAGAGCACACGTCTGAAC16012513.6561940775332773Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTGTTTAAATATTCTGTTGATGAACTCAGCAGATCGGAAGAGCACACGTC5269621.2032313131951773No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT3825740.8735449926452605No Hit
GAGGTCCGGACTTGGGGAGGGTTATCTACTAGATCGGAAGAGCACACGTC3416050.7799989994421582No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGT3082640.7038702933623262No Hit
TCAGGTCTAGGCTGGTTAGTTTAGATCGGAAGAGCACACGTCTGAACTCC2783050.6354638296856013Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTGTTTAAATATTCTGTTGATGAACTCAGTAGATCGGAAGAGCACACGTC2550000.5822506838534282No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTGGTATGCCGTC2182230.4982764352256731TruSeq Adapter, Index 2 (98% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTGGTATG1846230.4215563451179273TruSeq Adapter, Index 2 (97% over 49bp)
TGACCACTTTGGATTCTTGCGCCATTCTTAGATCGGAAGAGCACACGTCT1839670.42005847669201424Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GATTCTATAGACACCCCTCCTCCAAATACTTGACCTCTTTAGATCGGAAG1707780.38994355798870883No Hit
GAGGTCCGGACGGTCGAGGGTTATCATCCTTAGATCGGAAGAGCACACGT1533050.3500468277966659No Hit
AACTTTTGACGGTGGATCTCTTGGCTCACGTATCGATGAAGAACGCAGCC1478790.337657446578671No Hit
CTTCGGTACGCCCCTCCCCGCCTTCATTGACCTCATTAGATCGGAAGAGC1354300.30923219652654815No Hit
TCCCGTAGATCCGAACTTGTAGATCGGAAGAGCACACGTCTGAACTCCAG1342990.3066497434934571Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GAGGTCCGGACGGAGGAGGGTTATCCACGATTCTAGATCGGAAGAGCACA1254540.286453636439796No Hit
GTTGCTACGATCTTCTGAGATTAAGCCTTTGTTCTAAGATTTGTAGATCG1151190.2628553587236188No Hit
CTTCGGTACGCCCCTCCCCGCCTTCATTGACCTCATTTAGATCGGAAGAG989230.22587444862287323No Hit
GGTGACCGCCTGGGAATACCCCGTGTTGTAGGCTTAGATCGGAAGAGCAC985990.22513464775397712No Hit
TTGCTACGATCTTCTGAGATTAAGCCTTTGTTCTAAGATTTGTAGATCGG979450.22364134600009422No Hit
TGATTAGATCTAATGGTTTTTGAGCAAATTAGATCGGAAGAGCACACGTC963560.22001312507208207No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTAGTGTG918520.20972897966001994TruSeq Adapter, Index 2 (97% over 46bp)
GAGGTCCGGACGGAGGAGGGTTATCCACAGATCGGAAGAGCACACGTCTG827760.18900542198686812Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTGACCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTAT804670.1837331991279757TruSeq Adapter, Index 2 (100% over 41bp)
GAGGTCCGGACTTGGGGAGGGTTATCTACTACTAGATCGGAAGAGCACAC794520.18141561307263757No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTC767950.17534878928048633No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTAGTATGTCGTC719460.16427689294321077TruSeq Adapter, Index 2 (97% over 45bp)
GAGGTCCGGATTTGGGGAGGGTTATCTACTAGATCGGAAGAGCACACGTC676870.15455216485485096No Hit
CGATTCTATAGACACCCCTCCTCCAAATACTTGACCTCTTTAGATCGGAA668920.15273691272283732No Hit
TAAAATGTAAAATATGACGCTCCTAAAAGGAGATCGGAAGAGCACACGTC666330.15214552869492345No Hit
CCACGATTCTATAGACACCCCTCCTCCAAATACTTGACCTCTTTAGATCG632850.14450091971633022No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGTC627320.14323823489997356No Hit
GAGGTCCGGACTTGGGGAGGGTTATCTACCAGATCGGAAGAGCACACGTC619050.14134991601547636No Hit
CTTCGGTACGCCCCTCCCCGCCTTCATTGACCTCATTTTAGATCGGAAGA617130.14091151550057496No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTAGTGTGCCGTC586080.1338217571736538TruSeq Adapter, Index 2 (97% over 42bp)
TTCAACGTGACAACCTGTATGGCTTCCATTAGATCGGAAGAGCACACGTC580640.13257962238143317No Hit
TTTGCTCTTTCAACACTTCTAACTTTGTAGATCGGAAGAGCACACGTCTG577420.13184438818456726Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCAAGTCTAGGCTGGTTAGTTTAGATCGGAAGAGCACACGTCTGAACTCC563000.1285518176507765Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GAGGTCCGGACGGAGGAGGGTTATCCACGATTCAGATCGGAAGAGCACAC542200.1238024787393446No Hit
GAGGTCCGGACGGAGGAGGGTTATCCACGATTAGATCGGAAGAGCACACG510080.11646840345880653No Hit
GGGGACTAGTTGGGAAGTGCTTAGGGTACTGTCGGCTAGATCGGAAGAGC489040.11166426448301198No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTGGTGTG480300.10966862880580452TruSeq Adapter, Index 2 (97% over 46bp)
GCACTGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACG473380.10808856028334739No Hit
TCAGGTCTAGGCTGGTTAGTTCAGATCGGAAGAGCACACGTCTGAACTCC456030.10412697229712897Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTAGGATG450880.10295105424934654TruSeq Adapter, Index 2 (97% over 45bp)
GAGGTCCGGACTTGGGGAGGGTTATCTACTACTTAGATCGGAAGAGCACA446410.10193040305059171No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)