FastQCFastQC Report
Wed 30 Apr 2025
1G10_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1G10_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39812148
Total Bases5.9 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC68

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAAC691048917.357739652731123No Hit
AGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAAC595727014.96344784009142No Hit
GGCGAGAATTCTACCACTGAACCACAGTGCGATCGTCGGACTGTAGAACT10713212.690939961340443No Hit
AGCGAGAATTCTACCACTGAACCACAGTGCGATCGTCGGACTGTAGAACT9566992.4030328632356133No Hit
TGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAAC6135631.5411451801093476No Hit
TCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATCATT3415250.8578411795314335Illumina RNA PCR Primer (100% over 50bp)
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA3256790.8180392577662476Illumina RNA PCR Primer (100% over 45bp)
TTCCACTTCAGAGCAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA2877880.7228647899128678Illumina DpnII expression Sequencing Primer (100% over 25bp)
GCTGAGTTCATCAACAGAATATTTAAACAAGATCGTCGGACTGTAGAACT2531550.6358737539104898No Hit
ACTGAGTTCATCAACAGAATATTTAAACAAGATCGTCGGACTGTAGAACT1651910.4149261175257361No Hit
GCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAACT1027670.25812975476731376No Hit
TGCGAGAATTCTACCACTGAACCACAGTGCGATCGTCGGACTGTAGAACT965440.24249884733674756No Hit
AGGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAA879350.22087479429645443No Hit
AGCGAGAATTCTACCACTGAACCACCAGTGTGATCGTCGGACTGTAGAAC868870.21824243193308734No Hit
GGCGAGAATTCTACCACTGAACCACCAGTGTGATCGTCGGACTGTAGAAC867500.21789831586077696No Hit
CGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAAC811460.20382221024597816No Hit
AGTAGATAACCCTCCCCAAGTCCGGACCTCGATCGTCGGACTGTAGAACT738460.18548609836374566No Hit
AAGGATGATAACCCTCGACCGTCCGGACCTCGATCGTCGGACTGTAGAAC723200.1816530974415146No Hit
ACGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAACT681030.17106085308434No Hit
GGCGAGAATTCTACCACTGAACCACCAGTGCGATCGGTCGGACTGTAGAA670120.16832048348659812No Hit
ACAAATCTTAGAACAAAGGCTTAATCTCAGAAGATCGTAGCAACGATCGT666700.16746144920389625No Hit
ACAAATCTTAGAACAAAGGCTTAATCTCAGAAGATCGTAGCAAGATCGTC653320.16410066595753636No Hit
GGCGAGAATTCTACCACTGAACCACAAGTGCGATCGTCGGACTGTAGAAC617030.15498535773553337No Hit
ACCCGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCTGAAC612420.15382741971118966Illumina DpnII expression Sequencing Primer (100% over 26bp)
AAACTAACCAGCCTAGACCTGAGATCGTCGGACTGTAGAACTCTGAACGT586560.1473319148718125Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGCGAGAATTCTACCACTGAACCACCAGTGCGATCGGTCGGACTGTAGAA583040.14644776262762813No Hit
AGCGAGAATTCTACCACTGAACCACAAGTGCGATCGTCGGACTGTAGAAC547760.13758614581659848No Hit
TCCGACACTCAGACAGACATGCTCCTGGGAGAACCCAAGAGCGCCATTTG516840.1298196721262063No Hit
AAGCCTACAACACGGGGTATTCCCAGGCGGTCACCGATCGTCGGACTGTA514620.12926205338129457No Hit
ACTCTACAACTTACAATACCCCGTTCTTTATGATCGTCGGACTGTAGAAC473180.1188531701429423No Hit
GCCTGCACCCCACACCAGTGAGGCTCATCCGCGCGATCGTCGGACTGTAG459840.11550243408117541No Hit
ACTCTACAACTTACAATACCCCGTTCTGATCGTCGGACTGTAGAACTCTG447230.11233505913823087Illumina DpnII expression Sequencing Primer (100% over 23bp)
AGCGGGGATACTCACCACTATACTACCGAGGAGATCGTCGGACTGTAGAA420830.10570391730684815No Hit
AATGAGGTCAAGTTAGTGGAAGAGGGGTGATTCATGAGTTTCGATCGTCG419110.10527188836934898No Hit
AGTCTCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAGA417070.10475948195510576No Hit
ACCCGCTTCAAGTCTCAGCCCGGATCGTCGGACTGTAGAACTCTGAACGT410650.1031469088279286Illumina DpnII expression Sequencing Primer (96% over 28bp)
AATGAGGTCAATGAAGGCGGGGAGGGGCGTACCGAAGGATCGTCGGACTG404780.10167248448890524No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG400970.1007154901564216No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)