FastQCFastQC Report
Wed 30 Apr 2025
1G10_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1G10_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39812148
Total Bases5.9 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC67

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCACTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT686186717.23561110041086No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGT605067615.198064671114958No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTC10602502.663131866183156No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGTC9705642.437858916831114No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCAAGATCGGAAGAGCACACGT6032071.5151330191980599No Hit
TGAAAATCTTTGCTCTGAAGTGGAAAGATCGGAAGAGCACACGTCTGAAC2701110.6784637694002343Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTGTTTAAATATTCTGTTGATGAACTCAGCAGATCGGAAGAGCACACGTC2455770.6168393626990436No Hit
TTGTTTAAATATTCTGTTGATGAACTCAGTAGATCGGAAGAGCACACGTC1645380.41328591464092823No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTC1008470.25330710616267177No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCAAGATCGGAAGAGCACACGTC946960.23785704805477964No Hit
ACACTGGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGT928470.2332127369766635No Hit
ACACTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT908930.22830468730298098No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACG886740.22273101165001197No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCGAGATCGGAAGAGCACACGT765410.19225538898328218No Hit
GAGGTCCGGACTTGGGGAGGGTTATCTACTAGATCGGAAGAGCACACGTC736290.18494103859957517No Hit
GAGGTCCGGACGGTCGAGGGTTATCATCCTTAGATCGGAAGAGCACACGT727320.182687957454594No Hit
AACTTTTGACGGTGGATCTCTTGGCTCACGTATCGATGAAGAACGCAGCC711070.17860628871368608No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGTAGATCGGAAGAGCACACGTC685210.17211078387430892No Hit
GTTGCTACGATCTTCTGAGATTAAGCCTTTGTTCTAAGATTTGTAGATCG670250.16835313683652536No Hit
TTGCTACGATCTTCTGAGATTAAGCCTTTGTTCTAAGATTTGTAGATCGG657680.16519580907817383No Hit
CTCGGGCTGAGACTTGAAGCGGGTAGATCGGAAGAGCACACGTCTGAACT613380.15406855214142176Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GCACTTGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT610900.1534456266966555No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCATCTGGGGGG581540.14607099320539046TruSeq Adapter, Index 18 (97% over 45bp)
TCAGGTCTAGGCTGGTTAGTTTAGATCGGAAGAGCACACGTCTGAACTCC578960.1454229497991417Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCACTTGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGT556410.13975884948483563No Hit
GGTGACCGCCTGGGAATACCCCGTGTTGTAGGCTTAGATCGGAAGAGCAC512870.12882248905535065No Hit
ATAAAGAACGGGGTATTGTAAGTTGTAGAGTAGATCGGAAGAGCACACGT473820.11901392509643036No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCATCTAGGGTG460230.11560039413095723TruSeq Adapter, Index 18 (97% over 45bp)
GCGCGGATGAGCCTCACTGGTGTGGGGTGCAGGCAGATCGGAAGAGCACA457260.11485439067492666No Hit
AGAACGGGGTATTGTAAGTTGTAGAGTAGATCGGAAGAGCACACGTCTGA445620.11193065995836246Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCACTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAGAGCACACGTC425570.10689450868111915No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTAGATCGGAAGAGCACAC421580.10589230201796698No Hit
GAAACTCATGAATCACCCCTCTTCCACTAACTTGACCTCATTAGATCGGA420850.10570894089914466No Hit
TCCTCGGTAGTATAGTGGTGAGTATCCCCGCTAGATCGGAAGAGCACACG408190.10252900697545884No Hit
CGGGCTGAGACTTGAAGCGGGTAGATCGGAAGAGCACACGTCTGAACTCC406560.10211958420329394Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTTCGGTACGCCCCTCCCCGCCTTCATTGACCTCATTAGATCGGAAGAGC404320.10155694186608571No Hit
GCACTAGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT400950.1007104665641251No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)