FastQCFastQC Report
Wed 30 Apr 2025
1F7_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1F7_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3607654
Total Bases541.1 Mbp
Sequences flagged as poor quality0
Sequence length150
%GC67

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCAGACAACTACTCACGCTTAAGCACACGATCGTCGGACTGTAGAACTC2686837.447582279231877Illumina DpnII expression Sequencing Primer (100% over 21bp)
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA2557567.08926077722531Illumina RNA PCR Primer (100% over 45bp)
TTCCACTTCAGAGCAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA1977215.480597640461086Illumina DpnII expression Sequencing Primer (100% over 25bp)
AGACAACTACTCACGCTTAAGCACACGATCGTCGGACTGTAGAACTCTGA1580524.381018800583426Illumina DpnII expression Sequencing Primer (100% over 24bp)
AAACACAGCAGACAACTACTCACGCTTAAGCACACGATCGTCGGACTGTA760412.1077686496543184No Hit
ACAAATCTTAGAACAAAGGCTTAATCTCAGAAGATCGTAGCAACGATCGT559151.5498991865627911No Hit
TCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATCATT456301.264810871552538Illumina RNA PCR Primer (100% over 50bp)
GGCAGACAACTACTCACGCTTAAGCACACGATCGTCGGACTGTAGAACTC450991.2500921651577452Illumina DpnII expression Sequencing Primer (100% over 21bp)
ACAAATCTTAGAACAAAGGCTTAATCTCAGAAGATCGTAGCAAGATCGTC422551.1712597715856343No Hit
GGACAACTACTCACGCTTAAGCACACGATCGTCGGACTGTAGAACTCTGA227510.6306314297324521Illumina DpnII expression Sequencing Primer (100% over 24bp)
ACAAATCTTAGAACAAAGGCTTAATCTCAGAAGATCGTAGCGATCGTCGG169410.46958494356720465No Hit
GCTTTAAGAAAAGACACACCTCACAATTATACGATCGTCGGACTGTAGAA127250.3527222954307702No Hit
AGCCGACAGTACCCTAAGCACTTCCCAACTAGTCCCCGATCGTCGGACTG124120.34404629712272855No Hit
GCTGAGTTCATCAACAGAATATTTAAACAAGATCGTCGGACTGTAGAACT121950.338031307880412No Hit
ACAAATCTTAGAACAAAGGCTTAATCTCAGAAGATCGTAGCAGATCGTCG115390.3198477459312894No Hit
GCAGACAACTACTCACGCTTAAGCACACGATCGTCGGACTGTAGAACTCT107520.29803301536122917Illumina DpnII expression Sequencing Primer (100% over 22bp)
AGTAGATAACCCTCCCCAAGTCCGGACCTCGATCGTCGGACTGTAGAACT103010.2855318165212074No Hit
TGCAGACAACTACTCACGCTTAAGCACACGATCGTCGGACTGTAGAACTC95020.2633844598179315Illumina DpnII expression Sequencing Primer (100% over 21bp)
AATGAGGTCAATGAAGGCGGGGAGGGGCGTACCGAAGGATCGTCGGACTG88650.24572755591306705No Hit
GCAAATCTTAGAACAAAGGCTTAATCTCAGAAGATCGTAGCAACGATCGT77930.21601295467913498No Hit
GAACACAGCAGACAACTACTCACGCTTAAGCACACGATCGTCGGACTGTA75020.20794677094865527No Hit
TCCACTTCAGAGCAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAAC68940.1910937135323953Illumina DpnII expression Sequencing Primer (100% over 26bp)
ACAGACAACTACTCACGCTTAAGCACACGATCGTCGGACTGTAGAACTCT68920.191038275843526Illumina DpnII expression Sequencing Primer (100% over 22bp)
ACAAATCTTAGAACAAAGGCTTAATCTCAGAAGATCGTAGCAACAGATCG62040.171967710872495No Hit
GGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAAC61250.16977792216215856No Hit
GCAAATCTTAGAACAAAGGCTTAATCTCAGAAGATCGTAGCAAGATCGTC60050.16645166083000199No Hit
TATCATCAGAGGACAATTTCACATTCCTCACTAACAGTCAGATCGTCGGA59820.1658141274080053No Hit
TTTTTTTGGTCTTTCACCTACAGCATTAATAGATCGTCGGACTGTAGAAC56540.156722346433444No Hit
AAGTTCTTGGAGCCAACTTATTATGGACTTGTAGATCGTCGGACTGTAGA56020.15528096652284282No Hit
GGCAGAGTTGTGCACATTCATGCTGCCAGAGGTCACGATCGTCGGACTGT52760.1462446232371508No Hit
GAGGTCAAGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGG52240.14480324332654962Illumina RNA PCR Primer (100% over 37bp)
AACTGATTTGTAAGTAGTAGATAACCCTCCCCAAGTCCGGACCTCGATCG52130.1444983360377686No Hit
AATGAGGTCAAGTTAGTGGAAGAGGGGTGGATCGTCGGACTGTAGAACTC51630.1431123938160367Illumina DpnII expression Sequencing Primer (100% over 21bp)
TAAGAAACACAGCAGACAACTACTCACGCTTAAGCACACGATCGTCGGAC48180.13354939248608652No Hit
TTCCACTTCAGAGAAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA47030.13036172537610316Illumina DpnII expression Sequencing Primer (100% over 25bp)
ACTGAGTTCATCAACAGAATATTTAAACAAGATCGTCGGACTGTAGAACT46100.12778387284368178No Hit
ATCATCAGAGGACAATTTCACATTCCTCACTAACAGTCAGATCGTCGGAC45900.12722949595498903No Hit
AAGGATGATAACCCTCGACCGTCCGGACCTCGATCGTCGGACTGTAGAAC44590.12359832733405145No Hit
TTTTTTTTTCCTTCACGAGTAAACATTCTAGATCGTCGGACTGTAGAACT43300.12002259640198312No Hit
AGTAGTAGATAACCCTCCCCAAGTCCGGACCTCGATCGTCGGACTGTAGA42260.11713983658078075No Hit
CTGGCTGCGTTCTTCATCGATACGTGAGCCAAGAGATCCACCGTCAAAAG41700.11558758129244101No Hit
ATGAGGTCAAGTTAGTGGAAGAGGGGTGGATCGTCGGACTGTAGAACTCT40880.11331463604880068Illumina DpnII expression Sequencing Primer (100% over 22bp)
AAGTTTGAGAATAGGTCGAAGACAACACGCGATCGTCGGACTGTAGAACT40640.11264938378236938No Hit
AGCAGAGTTGTGCACATTCATGCTGCCAGAGGTCACGATCGTCGGACTGT39520.1095448732056899No Hit
GTCAAGTAGCTTCTGCTCAGCTCAAACATCAAGGATCGTCGGACTGTAGA38870.10774314831743842No Hit
GCTTTAAGAAAAGACACACCTCACAATTATACTGATCGTCGGACTGTAGA38780.10749367871752669No Hit
AAGCCTACAACACGGGGTATTCCCAGGCGGTCACCGATCGTCGGACTGTA38230.10596914227362159No Hit
ACCACCAAAGAGTCTAGAATTGTACATGATCGTCGGACTGTAGAACTCTG38070.10552564076266738Illumina DpnII expression Sequencing Primer (100% over 23bp)
AGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAAC38050.10547020307379809No Hit
AAAGAGGTCAAGTATTTGGAGGAGGGGTGTCTATAGAATCGATCGTCGGA37990.10530389000719027No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)