FastQCFastQC Report
Wed 30 Apr 2025
1F6_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1F6_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18903290
Total Bases2.8 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC67

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCCACTTCAGAGCAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA15047537.9602704079554405Illumina DpnII expression Sequencing Primer (100% over 25bp)
GGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAAC2316071.2252205832952887No Hit
ACAAATCTTAGAACAAAGGCTTAATCTCAGAAGATCGTAGCAACGATCGT1671200.884078909015309No Hit
ACAAATCTTAGAACAAAGGCTTAATCTCAGAAGATCGTAGCAAGATCGTC1567810.8293847261508447No Hit
AATGAGGTCAATGAAGGCGGGGAGGGGCGTACCGAAGGATCGTCGGACTG870060.4602690854343345No Hit
AAGCCTACAACACGGGGTATTCCCAGGCGGTCACCGATCGTCGGACTGTA810880.42896236581039593No Hit
GCTGAGTTCATCAACAGAATATTTAAACAAGATCGTCGGACTGTAGAACT714710.3780876239003898No Hit
AGGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAA583580.30871874684248085No Hit
CTGGCTGCGTTCTTCATCGATACGTGAGCCAAGAGATCCACCGTCAAAAG558310.2953507035018772No Hit
AATTTGCTCAAAAACCATTAGATCTAATCAGATCGTCGGACTGTAGAACT528050.2793429080334693No Hit
CCTGATCCTTGTCCATTGTATGAGATCGTCGGACTGTAGAACTCTGAACG526050.27828489114857785Illumina Small RNA Adapter 1 (100% over 26bp)
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA523390.27687772869167215Illumina RNA PCR Primer (100% over 45bp)
AAACTAACCAGCCTAGACCTGAGATCGTCGGACTGTAGAACTCTGAACGT515650.27278320334714223Illumina DpnII expression Sequencing Primer (96% over 28bp)
ACAAATCTTAGAACAAAGGCTTAATCTCAGAAGATCGTAGCAACAGATCG497130.2629859669930472No Hit
TCCGACACTCAGACAGACATGCTCCTGGGAGAACCCAAGAGCGCCATTTG472310.24985597745154414No Hit
CTCCACTTCAGAGCAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA410130.2169622325002685Illumina DpnII expression Sequencing Primer (100% over 25bp)
TCCGACACTCAGACAGACATGCTCCTGGGGATCGTCGGACTGTAGAACTC392440.20760407315340346Illumina DpnII expression Sequencing Primer (100% over 21bp)
TCCACTTCAGAGCAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAAC377090.19948379356186144Illumina DpnII expression Sequencing Primer (100% over 26bp)
GGCGAGAATTCTACCACTGAACCACAGTGCGATCGTCGGACTGTAGAACT350780.1855655814411142No Hit
AGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAAC332820.17606458981478884No Hit
TACTAATGAGGGATAACCCTCCCCTGAGTTCCGGACCTCGATCGTCGGAC319280.16890181550407363No Hit
TACCACTTCAGAGCAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA317230.16781734819705987Illumina DpnII expression Sequencing Primer (100% over 25bp)
AATGGAAGCCATACAGGTTGTCACGTTGAAGATCGTCGGACTGTAGAACT305670.1617020106023872No Hit
TTTTTTTGGTCTTTCACCTACAGCATTAATAGATCGTCGGACTGTAGAAC283820.15014317613494796No Hit
GTCTCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAGAA281430.14887884595750261No Hit
GCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAACT278830.14750342400714375No Hit
AGTAGATAACCCTCCCCAAGTCCGGACCTCGATCGTCGGACTGTAGAACT276220.14612271197236038No Hit
AAAATGAGGTCAATGAAGGCGGGGAGGGGCGTACCGAAGGATCGTCGGAC273160.14450394613847645No Hit
ACAAATCTTAGAACAAAGGCTTAATCTCAGAAGATCGTAGCAACAAGGCT267570.14154678894520478No Hit
AAGGATGATAACCCTCGACCGTCCGGACCTCGATCGTCGGACTGTAGAAC256430.13565363489635932No Hit
ACAAGTTCGGATCTACGGGAGATCGTCGGACTGTAGAACTCTGAACGTGT251110.132839309982548Illumina DpnII expression Sequencing Primer (96% over 30bp)
ACAAATCTTAGAACAAAGGCTTAATCTCAGAAGATCGTAGCAACAAGATC246740.13052754308906017No Hit
TCCCACTTCAGAGCAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA245120.12967054941229808Illumina DpnII expression Sequencing Primer (100% over 25bp)
GTGGATAACCCTCCTCCGTCCGGACCTCGATCGTCGGACTGTAGAACTCT238810.1263325061404655Illumina DpnII expression Sequencing Primer (100% over 22bp)
ACAAATCTTAGAACAAAGGCTTAATCTCAGAAGATCGTAGCAGATCGTCG231000.12220095020496433No Hit
GAGGTCAAGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGG228220.12073030673496518Illumina RNA PCR Primer (100% over 37bp)
TCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATCATT226950.1200584660130591Illumina RNA PCR Primer (100% over 50bp)
TCCGACACTCAGACAGACATGCTCCTGGGAGAGATCGTCGGACTGTAGAA225300.11918560208302365No Hit
CCACTTCAGAGCAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAACG224470.1187465250757937Illumina Small RNA Adapter 1 (100% over 26bp)
AAATGAGGTCAATGAAGGCGGGGAGGGGCGTACCGAAGGATCGTCGGACT223690.11833389849068603No Hit
ACAAATCTTAGAACAAAGGCTTAATCTCAGATCGTCGGACTGTAGAACTC222580.11774669911957125Illumina DpnII expression Sequencing Primer (100% over 21bp)
ACACGTCAGATGCTGAACAGATGCTGATAGATCGTCGGACTGTAGAACTC217110.11485302293939309Illumina DpnII expression Sequencing Primer (100% over 21bp)
TCCGACACTCAGACAGACATGGATCGTCGGACTGTAGAACTCTGAACGTG213170.11276872967615689Illumina DpnII expression Sequencing Primer (96% over 29bp)
CCACAAGTTCGGATCTACGGGAGATCGTCGGACTGTAGAACTCTGAACGT211030.11163665160932304Illumina DpnII expression Sequencing Primer (96% over 28bp)
GTCTGATGCTCTACCGACTGAGCTAACCGGGCGATCGTCGGACTGTAGAA206430.10920321277407266No Hit
AGAACCGGTGTTCTCCCTGTGGTATGGTCGTAGGCGATCGTCGGACTGTA193380.10229965260015585No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)