FastQCFastQC Report
Wed 30 Apr 2025
1F6_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1F6_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18903290
Total Bases2.8 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC71

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAAAATCTTTGCTCTGAAGTGGAAAGATCGGAAGAGCACACGTCTGAAC13955777.382720150830887Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCACTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT2218531.173621099819132No Hit
GTTGCTACGATCTTCTGAGATTAAGCCTTTGTTCTAAGATTTGTAGATCG1642030.8686477327491668No Hit
TTGCTACGATCTTCTGAGATTAAGCCTTTGTTCTAAGATTTGTAGATCGG1544040.8168101954739095No Hit
AACTTTTGACGGTGGATCTCTTGGCTCACGTATCGATGAAGAACGCAGCC1051950.5564904310307889No Hit
CTTCGGTACGCCCCTCCCCGCCTTCATTGACCTCATTAGATCGGAAGAGC843610.4462768121316448No Hit
GGTGACCGCCTGGGAATACCCCGTGTTGTAGGCTTAGATCGGAAGAGCAC783140.41428767161695135No Hit
TTGTTTAAATATTCTGTTGATGAACTCAGCAGATCGGAAGAGCACACGTC634940.3358886204464937No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACG567350.3001329398215866No Hit
TGATTAGATCTAATGGTTTTTGAGCAAATTAGATCGGAAGAGCACACGTC504000.26662025499264946No Hit
TGTTGCTACGATCTTCTGAGATTAAGCCTTTGTTCTAAGATTTGTAGATC490960.25972198490315707No Hit
TCATACAATGGACAAGGATCAGGAGATCGGAAGAGCACACGTCTGAACTC485830.25700817159341044Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCAGGTCTAGGCTGGTTAGTTTAGATCGGAAGAGCACACGTCTGAACTCC482810.25541056609722435Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCCGAATCTGGGGGG457030.24177272845097336TruSeq Adapter, Index 6 (97% over 36bp)
TGAAAATCTTTGCTCTGAAGTGGAGAGATCGGAAGAGCACACGTCTGAAC383060.2026419739632625Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CCCAGGAGCATGTCTGTCTGAGTGTCGGAAGATCGGAAGAGCACACGTCT370420.19595530725074842Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGAAAATCTTTGCTCTGAAGTGGAAGATCGGAAGAGCACACGTCTGAACT349060.18465568692010756Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GCACTGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTC331890.17557261196331433No Hit
TGAAAATCTTTGCTCTGAAGTGGTAAGATCGGAAGAGCACACGTCTGAAC293720.15538035971516068Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTCAACGTGACAACCTGTATGGCTTCCATTAGATCGGAAGAGCACACGTC292020.15448104536300294No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGT290010.15341773839368703No Hit
GAGGTCCGGAACTCAGGGGAGGGTTATCCCTCATTAGTAAGATCGGAAGA278330.14723891978592085No Hit
TATTAATGCTGTAGGTGAAAGACCAAAAAAAAGATCGGAAGAGCACACGT271750.14375804423462793No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACAGATCGGAAGAGCACACG268000.14177426257545644No Hit
CTTCGGTACGCCCCTCCCCGCCTTCATTGACCTCATTTTAGATCGGAAGA265550.1404781918914644No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTC255900.13537326042186307No Hit
GAGGTCCGGACGGTCGAGGGTTATCATCCTTAGATCGGAAGAGCACACGT246320.13030535954323294No Hit
TTGTTGCTACGATCTTCTGAGATTAAGCCTTTGTTCTAAGATTTGTAGAT241960.12799888273416954No Hit
GAGGTCCGGACTTGGGGAGGGTTATCTACTAGATCGGAAGAGCACACGTC238950.1264065673224079No Hit
TCCCGTAGATCCGAACTTGTAGATCGGAAGAGCACACGTCTGAACTCCAG236720.1252268784957539Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TGAAAATCTTTGCTCTGAAGTGGGAAGATCGGAAGAGCACACGTCTGAAC229810.1215714301584539Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TGCTACGATCTTCTGAGATTAAGCCTTTGTTCTAAGATTTGTAGATCGGA228100.12066682572187169No Hit
CTTCGGTACGCCCCTCCCCGCCTTCATTGACCTCATTTAGATCGGAAGAG217110.11485302293939309No Hit
TCTCCCAGGAGCATGTCTGTCTGAGTGTCGGAAGATCGGAAGAGCACACG216150.11434517483464518No Hit
TGAGATTAAGCCTTTGTTCTAAGATTTGTAGATCGGAAGAGCACACGTCT212290.11230320224680466Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GAGGTCCGGACGGAGGAGGGTTATCCACAGATCGGAAGAGCACACGTCTG211350.11180593431090566Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGAAAATCTTTGCTCTGAAGTGGAGATCGGAAGAGCACACGTCTGAACTC206310.10913973176097919Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TATCAGCATCTGTTCAGCATCTGACGTGTAGATCGGAAGAGCACACGTCT206160.10906038049461231Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTGACCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCCGAAT205460.1086900745849003TruSeq Adapter, Index 6 (97% over 36bp)
GCCCGGTTAGCTCAGTCGGTAGAGCATCAGACAGATCGGAAGAGCACACG197240.10434162518799639No Hit
CATGTCTGTCTGAGTGTCGGAAGATCGGAAGAGCACACGTCTGAACTCCA196660.10403480029137785Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCCCGTAGATCCGAACTTGTGGAGATCGGAAGAGCACACGTCTGAACTCC193000.10209862939202646Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)