FastQCFastQC Report
Wed 30 Apr 2025
1F3_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1F3_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49934377
Total Bases7.4 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC67

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCCACTTCAGAGCAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA46951109.40256048453353Illumina DpnII expression Sequencing Primer (100% over 25bp)
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA23629084.732026595625695Illumina RNA PCR Primer (100% over 45bp)
AGCAGACAACTACTCACGCTTAAGCACACGATCGTCGGACTGTAGAACTC13374302.678375260394257Illumina DpnII expression Sequencing Primer (100% over 21bp)
AGACAACTACTCACGCTTAAGCACACGATCGTCGGACTGTAGAACTCTGA12081972.419569588301863Illumina DpnII expression Sequencing Primer (100% over 24bp)
ACAAATCTTAGAACAAAGGCTTAATCTCAGAAGATCGTAGCAACGATCGT11626422.3283398529233676No Hit
ACAAATCTTAGAACAAAGGCTTAATCTCAGAAGATCGTAGCAAGATCGTC8749781.752255765602122No Hit
TCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATCATT5110591.0234612519547406Illumina RNA PCR Primer (100% over 50bp)
GCTGAGTTCATCAACAGAATATTTAAACAAGATCGTCGGACTGTAGAACT4760530.9533572432474725No Hit
GGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAAC4018510.8047582129641869No Hit
ACAAATCTTAGAACAAAGGCTTAATCTCAGAAGATCGTAGCGATCGTCGG3750180.7510216859219051No Hit
AAACACAGCAGACAACTACTCACGCTTAAGCACACGATCGTCGGACTGTA2813460.5634314812819232No Hit
GGCAGACAACTACTCACGCTTAAGCACACGATCGTCGGACTGTAGAACTC2509270.5025135289061481Illumina DpnII expression Sequencing Primer (100% over 21bp)
AGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAAC2193810.43933861435780003No Hit
ACAAATCTTAGAACAAAGGCTTAATCTCAGAAGATCGTAGCAGATCGTCG2161270.432822061643024No Hit
GGACAACTACTCACGCTTAAGCACACGATCGTCGGACTGTAGAACTCTGA1829860.36645295484511603Illumina DpnII expression Sequencing Primer (100% over 24bp)
ACTGAGTTCATCAACAGAATATTTAAACAAGATCGTCGGACTGTAGAACT1727410.3459360271982566No Hit
GCAAATCTTAGAACAAAGGCTTAATCTCAGAAGATCGTAGCAACGATCGT1613730.3231701478923027No Hit
TCCACTTCAGAGCAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAAC1391140.27859364301270845Illumina DpnII expression Sequencing Primer (100% over 26bp)
AGTAGATAACCCTCCCCAAGTCCGGACCTCGATCGTCGGACTGTAGAACT1377410.27584403426120646No Hit
AATGAGGTCAATGAAGGCGGGGAGGGGCGTACCGAAGGATCGTCGGACTG1280320.25640051542046877No Hit
GCAAATCTTAGAACAAAGGCTTAATCTCAGAAGATCGTAGCAAGATCGTC1205710.2414589051546593No Hit
TTTTTTTGGTCTTTCACCTACAGCATTAATAGATCGTCGGACTGTAGAAC1181400.23659051558808875No Hit
ACAAGTTCGGATCTACGGGAGATCGTCGGACTGTAGAACTCTGAACGTGT1001230.20050916025246496Illumina DpnII expression Sequencing Primer (96% over 30bp)
TTCCACTTCAGAGAAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA994780.19921746495405362Illumina DpnII expression Sequencing Primer (100% over 25bp)
AGGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAA982950.1968483555927813No Hit
ATGAGGTCAAGTTAGTGGAAGAGGGGTGGATCGTCGGACTGTAGAACTCT980150.1962876196492849Illumina DpnII expression Sequencing Primer (100% over 22bp)
AATGAGGTCAAGTTAGTGGAAGAGGGGTGGATCGTCGGACTGTAGAACTC969210.19409674421290968Illumina DpnII expression Sequencing Primer (100% over 21bp)
ACAAATCTTAGAACAAAGGCTTAATCTCAGAAGATCGTAGCAACAGATCG954060.1910627622329202No Hit
AGCCGACAGTACCCTAAGCACTTCCCAACTAGTCCCCGATCGTCGGACTG949710.19019161889213115No Hit
AAACTAACCAGCCTAGACCTGAGATCGTCGGACTGTAGAACTCTGAACGT860310.17228812126763893Illumina DpnII expression Sequencing Primer (96% over 28bp)
AAGCCTACAACACGGGGTATTCCCAGGCGGTCACCGATCGTCGGACTGTA858310.17188759559371292No Hit
AAGGATGATAACCCTCGACCGTCCGGACCTCGATCGTCGGACTGTAGAAC813940.1630019335176646No Hit
CTGGCTGCGTTCTTCATCGATACGTGAGCCAAGAGATCCACCGTCAAAAG744110.14901757961253828No Hit
AAAGAGGTCAAGTATTTGGAGGAGGGGTGTCTATAGAATCGATCGTCGGA721530.14449564475391374No Hit
GAGGTCAAGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGG697150.13961323678875576Illumina RNA PCR Primer (100% over 37bp)
ACACTCTTCTTGGTGCAGGAGTCGACAGCTGATCGTCGGACTGTAGAACT602270.1206122988177063No Hit
GCTTTAAGAAAAGACACACCTCACAATTATACGATCGTCGGACTGTAGAA594720.11910031439863564No Hit
GCAGACAACTACTCACGCTTAAGCACACGATCGTCGGACTGTAGAACTCT579670.11608635870234248Illumina DpnII expression Sequencing Primer (100% over 22bp)
TCCCACTTCAGAGCAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA567840.11371724934107016Illumina DpnII expression Sequencing Primer (100% over 25bp)
AAAATGAGGTCAATGAAGGCGGGGAGGGGCGTACCGAAGGATCGTCGGAC540080.1081579529869773No Hit
AGTAGTAGATAACCCTCCCCAAGTCCGGACCTCGATCGTCGGACTGTAGA539870.10811589779121505No Hit
TATCATCAGAGGACAATTTCACATTCCTCACTAACAGTCAGATCGTCGGA535040.10714862828868377No Hit
GCAAATCTTAGAACAAAGGCTTAATCTCAGAAGATCGTAGCGATCGTCGG510940.10232229391787545No Hit
TGCAGACAACTACTCACGCTTAAGCACACGATCGTCGGACTGTAGAACTC509960.10212603633765172Illumina DpnII expression Sequencing Primer (100% over 21bp)
AAAGAGGTCAAGTATTTGGAGGAGGGGTGTCTATAGAATTGATCGTCGGA508180.10176956848785758No Hit
ACTTCAGAGCAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAACGTG502530.10063808345901662Illumina DpnII expression Sequencing Primer (96% over 29bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)