FastQCFastQC Report
Wed 30 Apr 2025
1F3_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1F3_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49934377
Total Bases7.4 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC64

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAAAATCTTTGCTCTGAAGTGGAAAGATCGGAAGAGCACACGTCTGAAC46366949.285574945693224Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GTGTGCTTAAGCGTGAGTAGTTGTCTGCTAGATCGGAAGAGCACACGTCT13427732.68907530377319Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTGGGGGG12111692.4255213998164034TruSeq Adapter, Index 8 (97% over 45bp)
GTGTGCTTAAGCGTGAGTAGTTGTCTAGATCGGAAGAGCACACGTCTGAA12096352.4224493678973906Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GTTGCTACGATCTTCTGAGATTAAGCCTTTGTTCTAAGATTTGTAGATCG11639622.3309833223712793No Hit
TTGCTACGATCTTCTGAGATTAAGCCTTTGTTCTAAGATTTGTAGATCGG8797071.7617261951621024No Hit
TTGTTTAAATATTCTGTTGATGAACTCAGCAGATCGGAAGAGCACACGTC4746860.9506196502661884No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT4110800.8232404701875022No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTGGGGTG3870950.7752074287419266TruSeq Adapter, Index 8 (97% over 45bp)
GCTACGATCTTCTGAGATTAAGCCTTTGTTCTAAGATTTGTAGATCGGAA3760180.753024314291535No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTAGGGGG3703680.7417094640031255TruSeq Adapter, Index 8 (97% over 45bp)
GTGTGCTTAAGCGTGAGTAGTTGTCTGCTGTGTTTAGATCGGAAGAGCAC2824330.5656083383197111No Hit
AACTTTTGACGGTGGATCTCTTGGCTCACGTATCGATGAAGAACGCAGCC2623610.5254115816844976No Hit
GTGTGCTTAAGCGTGAGTAGTTGTCTGCCAGATCGGAAGAGCACACGTCT2546770.5100233852922607Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCACTGGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGT2242910.4491715196526833No Hit
TGCTACGATCTTCTGAGATTAAGCCTTTGTTCTAAGATTTGTAGATCGGA2175830.4357378885492053No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTAGGGTG1876440.37578119779085256TruSeq Adapter, Index 8 (97% over 45bp)
GTGTGCTTAAGCGTGAGTAGTTGTCCAGATCGGAAGAGCACACGTCTGAA1858530.37219449038084523Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTGTTTAAATATTCTGTTGATGAACTCAGTAGATCGGAAGAGCACACGTC1725430.34553950678106987No Hit
GTTGCTACGATCTTCTGAGATTAAGCCTTTGTTCTAAGATTTGCAGATCG1597690.3199579319874162No Hit
GAGGTCCGGACTTGGGGAGGGTTATCTACTAGATCGGAAGAGCACACGTC1383100.2769835298035259No Hit
TGAAAATCTTTGCTCTGAAGTGGAAGATCGGAAGAGCACACGTCTGAACT1366870.27373326395961645Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTTCGGTACGCCCCTCCCCGCCTTCATTGACCTCATTAGATCGGAAGAGC1296640.2596688049197049No Hit
TTGCTACGATCTTCTGAGATTAAGCCTTTGTTCTAAGATTTGCAGATCGG1193210.23895561969262177No Hit
TATTAATGCTGTAGGTGAAAGACCAAAAAAAAGATCGGAAGAGCACACGT1182330.23677676002646433No Hit
TCCCGTAGATCCGAACTTGTAGATCGGAAGAGCACACGTCTGAACTCCAG1006170.20149845866706217Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GCACTGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACG1001410.20054520756311828No Hit
CACCCCTCTTCCACTAACTTGACCTCATAGATCGGAAGAGCACACGTCTG999820.2002267896523471Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CACCCCTCTTCCACTAACTTGACCTCATTAGATCGGAAGAGCACACGTCT994400.19914136507600766Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGAAAATCTTTTCTCTGAAGTGGAAAGATCGGAAGAGCACACGTCTGAAC980900.19643781677700714Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TGTTGCTACGATCTTCTGAGATTAAGCCTTTGTTCTAAGATTTGTAGATC953690.1909886649832439No Hit
GGGGACTAGTTGGGAAGTGCTTAGGGTACTGTCGGCTAGATCGGAAGAGC950220.1902937529389823No Hit
TCAGGTCTAGGCTGGTTAGTTTAGATCGGAAGAGCACACGTCTGAACTCC864530.17313323043962278Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGTGACCGCCTGGGAATACCCCGTGTTGTAGGCTTAGATCGGAAGAGCAC860700.17236622377405447No Hit
GAGGTCCGGACGGTCGAGGGTTATCATCCTTAGATCGGAAGAGCACACGT824670.1651507537582776No Hit
GATTCTATAGACACCCCTCCTCCAAATACTTGACCTCTTTAGATCGGAAG724290.1450483701839316No Hit
TTGACCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAAT694060.13899442462254008TruSeq Adapter, Index 8 (100% over 41bp)
AGCTGTCGACTCCTGCACCAAGAAGAGTGTAGATCGGAAGAGCACACGTC610640.1222884987630866No Hit
GTATAATTGTGAGGTGTGTCTTTTCTTAAAGCAGATCGGAAGAGCACACG595700.11929657197885937No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTGGGGGGGGGGG592780.11871180449492741TruSeq Adapter, Index 8 (97% over 41bp)
GTGTGCTTAAGCGTGAGTAGTTGTCTGCAGATCGGAAGAGCACACGTCTG577120.11557568846808683Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGAAAATCTTTGCTCTGAAGTGGGAAGATCGGAAGAGCACACGTCTGAAC559660.11207909933471283Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CTTCGGTACGCCCCTCCCCGCCTTCATTGACCTCATTTTAGATCGGAAGA542210.10858451282970848No Hit
GAGGTCCGGACTTGGGGAGGGTTATCTACTACTAGATCGGAAGAGCACAC542190.10858050757296923No Hit
TGACTGTTAGTGAGGAATGTGAAATTGTCCTCTGATGATAAGATCGGAAG530870.10631353225854805No Hit
AATTCTATAGACACCCCTCCTCCAAATACTTGACCTCTTTAGATCGGAAG514560.10304724538768152No Hit
GTGTGCTTAAGCGTGAGTAGTTGTCTGCAAGATCGGAAGAGCACACGTCT511240.10238237276896435Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCTACGATCTTCTGAGATTAAGCCTTTGTTCTAAGATTTGCAGATCGGAA508690.1018717025347087No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)