FastQCFastQC Report
Wed 30 Apr 2025
1F1_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1F1_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences436846
Total Bases65.5 Mbp
Sequences flagged as poor quality0
Sequence length150
%GC68

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA7137616.338938664884193Illumina RNA PCR Primer (100% over 45bp)
ACAAATCTTAGAACAAAGGCTTAATCTCAGAAGATCGTAGCAAGATCGTC237945.446770715538199No Hit
TTCCACTTCAGAGCAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA162183.712521117281605Illumina DpnII expression Sequencing Primer (100% over 25bp)
ACAAATCTTAGAACAAAGGCTTAATCTCAGAAGATCGTAGCAACGATCGT139143.1851041328065266No Hit
TCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATCATT138563.171827142745956Illumina RNA PCR Primer (100% over 50bp)
GGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAAC50271.150748776456692No Hit
ACAAATCTTAGAACAAAGGCTTAATCTCAGAAGATCGTAGCGATCGTCGG37910.8678115399934988No Hit
ACAAATCTTAGAACAAAGGCTTAATCTCAGAAGATCGTAGCAGATCGTCG25880.5924284530475271No Hit
GCAAATCTTAGAACAAAGGCTTAATCTCAGAAGATCGTAGCAAGATCGTC24480.5603805460047706No Hit
ACCCGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCTGAAC24230.5546577054614212Illumina DpnII expression Sequencing Primer (100% over 26bp)
AGTAGATAACCCTCCCCAAGTCCGGACCTCGATCGTCGGACTGTAGAACT22270.5097906356015621No Hit
AAAGAGGTCAAGTATTTGGAGGAGGGGTGTCTATAGAATCGATCGTCGGA19250.4406587218379017No Hit
ACCCGCTTCAAGTCTCAGCCCGAGAGATCGTCGGACTGTAGAACTCTGAA18540.4244058546947894Illumina DpnII expression Sequencing Primer (100% over 25bp)
CTGGCTGCGTTCTTCATCGATACGTGAGCCAAGAGATCCACCGTCAAAAG18210.41685170517756825No Hit
AGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAAC17460.3996831835475202No Hit
GAGGTCAAGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGG16730.38297248916094Illumina RNA PCR Primer (100% over 37bp)
ACCCGCTTCAAGTCTCGATCGTCGGACTGTAGAACTCTGAACGTGTAGAT16310.37335811704811306Illumina DpnII expression Sequencing Primer (96% over 30bp)
GCAAATCTTAGAACAAAGGCTTAATCTCAGAAGATCGTAGCAACGATCGT14200.32505734286224436No Hit
ACAAATCTTAGAACAAAGGCTTAATCTCAGAAGATCGTAGCAACAGATCG14050.32162363853623477No Hit
AATTCAGAAAAACTGAACAGTCATCAGTGCTGATCGTCGGACTGTAGAAC12580.2879733361413404No Hit
ATGAGGTCAAGTTAGTGGAAGAGGGGTGGATCGTCGGACTGTAGAACTCT11900.27240720986343014Illumina DpnII expression Sequencing Primer (100% over 22bp)
CCGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCTGAACGT11890.27217829624169615Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGTAGTAGATAACCCTCCCCAAGTCCGGACCTCGATCGTCGGACTGTAGA9340.21380532269953256No Hit
AATGAGGTCAATGAAGGCGGGGAGGGGCGTACCGAAGGATCGTCGGACTG9330.2135764090777986No Hit
AGGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAA9190.21037161837352295No Hit
AGAATCGTGGATAACCCTCCTCCGTCCGGACCTCGATCGTCGGACTGTAG8970.20533551869537547No Hit
AAGAGGTCAAGTATTTGGAGGAGGGGTGTCTATAGAATCGATCGTCGGAC8580.19640788744775045No Hit
AAAGAGGTCAAGTATTTGGAGGAGGGGTGTCTATAGAATCGGATCGTCGG8240.18862482430879532No Hit
AATGAGGTCAAGTTAGTGGAAGAGGGGTGGATCGTCGGACTGTAGAACTC8170.1870224289566575Illumina DpnII expression Sequencing Primer (100% over 21bp)
CCGCTTCAAGTCTCGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCT7750.17740805684383054Illumina RNA PCR Primer (100% over 31bp)
TAGTAGATAACCCTCCCCAAGTCCGGACCTCGATCGTCGGACTGTAGAAC7230.16550454851366386No Hit
AATGAGGTCAAGTTAGTGGAAGAGGGGTGATTCATGAGTTTCGATCGTCG6630.15176973120962534No Hit
ATCATCAGAGGACAATTTCACATTCCTCACTAACAGTCAGATCGTCGGAC6320.14467340893587213No Hit
ACAAGTTCGGATCTACGGGAGATCGTCGGACTGTAGAACTCTGAACGTGT6210.1421553590967984Illumina DpnII expression Sequencing Primer (96% over 30bp)
AGTGAGGTCTAAGAGCGGGGAGGGGGGAGATCAACTGATTTGATCGTCGG5940.13597469130998108No Hit
GTGGATAACCCTCCTCCGTCCGGACCTCGATCGTCGGACTGTAGAACTCT5760.13185424611876956Illumina DpnII expression Sequencing Primer (100% over 22bp)
AAGTAGTAGATAACCCTCCCCAAGTCCGGACCTCGATCGTCGGACTGTAG5740.1313964188753016No Hit
AAAGAGGTCAAGTATTTGGAGGAGGGGTGTCTATAGAATCGTGATCGTCG5630.12887836903622787No Hit
AAGGATGATAACCCTCGACCGTCCGGACCTCGATCGTCGGACTGTAGAAC5360.12269770124941055No Hit
GCTGAGTTCATCAACAGAATATTTAAACAAGATCGTCGGACTGTAGAACT5170.11834834243646504No Hit
TCCGACACTCAGACAGACATGCTCCTGGGAGAACCCAAGAGCGCCATTTG5100.1167459470843272No Hit
ACCCGCTTCAAGTCTCAGCCCGGATCGTCGGACTGTAGAACTCTGAACGT5100.1167459470843272Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGTAGATAACCCTCCCCAAATCCGGACCTCGATCGTCGGACTGTAGAACT5030.11514355173218938No Hit
AACTGATTTGTAAGTAGTAGATAACCCTCCCCAAGTCCGGACCTCGATCG5010.11468572448872143No Hit
TCCACTTCAGAGCAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAAC4850.11102310654097783Illumina DpnII expression Sequencing Primer (100% over 26bp)
TGTCTTTCACCTACAGCATTAATAGATCGTCGGACTGTAGAACTCTGAAC4830.11056527929750987Illumina DpnII expression Sequencing Primer (100% over 26bp)
AAACTAACCAGCCTAGACCTGAGATCGTCGGACTGTAGAACTCTGAACGT4830.11056527929750987Illumina DpnII expression Sequencing Primer (96% over 28bp)
AAGAGGTCAAGTATTTGGAGGAGGGGTGGATCGTCGGACTGTAGAACTCT4490.10278221615855473Illumina DpnII expression Sequencing Primer (100% over 22bp)
CAGCCCAGGCCGGCTCCACGATCGTCGGACTGTAGAACTCTGAACGTGTA4470.10232438891508679Illumina DpnII expression Sequencing Primer (96% over 30bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)