FastQCFastQC Report
Wed 30 Apr 2025
1F1_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1F1_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences436846
Total Bases65.5 Mbp
Sequences flagged as poor quality0
Sequence length150
%GC68

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTGGGGGG6673515.276550546416814TruSeq Adapter, Index 1 (97% over 45bp)
TTGCTACGATCTTCTGAGATTAAGCCTTTGTTCTAAGATTTGTAGATCGG240245.499420848537013No Hit
TGAAAATCTTTGCTCTGAAGTGGAAAGATCGGAAGAGCACACGTCTGAAC158643.6314856951877776Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GTTGCTACGATCTTCTGAGATTAAGCCTTTGTTCTAAGATTTGTAGATCG140213.2095978903320623No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTGGGGGGGGGGG79811.8269596150588536TruSeq Adapter, Index 1 (97% over 41bp)
GCACTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT51741.1843990788515861No Hit
GCTACGATCTTCTGAGATTAAGCCTTTGTTCTAAGATTTGTAGATCGGAA38050.8710163306977745No Hit
AACTTTTGACGGTGGATCTCTTGGCTCACGTATCGATGAAGAACGCAGCC29950.6855962970932548No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTAGGGGG26690.610970456407979TruSeq Adapter, Index 1 (97% over 45bp)
TGCTACGATCTTCTGAGATTAAGCCTTTGTTCTAAGATTTGTAGATCGGA26210.5999826025647482No Hit
CTCGGGCTGAGACTTGAAGCGGGTAGATCGGAAGAGCACACGTCTGAACT24110.5519107420006134Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTGCTACGATCTTCTGAGATTAAGCCTTTGTTCTAAGATTTGCAGATCGG23830.5455011605920622No Hit
GAGGTCCGGACTTGGGGAGGGTTATCTACTAGATCGGAAGAGCACACGTC21610.4946823365671198No Hit
GATTCTATAGACACCCCTCCTCCAAATACTTGACCTCTTTAGATCGGAAG19330.4424900308117735No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTGGGGGGGGGGT18860.43173109059027664TruSeq Adapter, Index 1 (97% over 41bp)
TCTCGGGCTGAGACTTGAAGCGGGTAGATCGGAAGAGCACACGTCTGAAC18420.42165889123398176Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTGACCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGAT16330.373815944291581TruSeq Adapter, Index 1 (100% over 41bp)
GCACTGGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGT16090.3683220173699656No Hit
GAGACTTGAAGCGGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC16020.3667196220178278Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TGTTGCTACGATCTTCTGAGATTAAGCCTTTGTTCTAAGATTTGTAGATC14060.3218525521579687No Hit
GTTGCTACGATCTTCTGAGATTAAGCCTTTGTTCTAAGATTTGCAGATCG13990.32025015680583085No Hit
AGCACTGATGACTGTTCAGTTTTTCTGAATTAGATCGGAAGAGCACACGT12790.2927805221977539No Hit
CACCCCTCTTCCACTAACTTGACCTCATAGATCGGAAGAGCACACGTCTG11850.2712626417547602Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTCGGGCTGAGACTTGAAGCGGAGATCGGAAGAGCACACGTCTGAACTCC11270.2579856516941897Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTTCGGTACGCCCCTCCCCGCCTTCATTGACCTCATTAGATCGGAAGAGC9390.21494989080820243No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACG9320.2133474954560646No Hit
GAGGTCCGGACGGAGGAGGGTTATCCACGATTCTAGATCGGAAGAGCACA8910.20396203696497164No Hit
GAGGTCCGGACTTGGGGAGGGTTATCTACTACTAGATCGGAAGAGCACAC8740.20007050539549406No Hit
GATTCTATAGACACCCCTCCTCCAAATACTTGACCTCTTAGATCGGAAGA8570.1961789738260165No Hit
CACCCCTCTTCCACTAACTTGACCTCATTAGATCGGAAGAGCACACGTCT8510.19480549209561263Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGATTCTATAGACACCCCTCCTCCAAATACTTGACCTCTTTAGATCGGAA8300.18999830603919915No Hit
GAGGTCCGGACTTGGGGAGGGTTATCTACTAAGATCGGAAGAGCACACGT7090.16229975780938818No Hit
GAGACTTGAAGCGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAT7070.16184193056592028TruSeq Adapter, Index 1 (100% over 35bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTGGGGTG6820.1561190900225709TruSeq Adapter, Index 1 (97% over 45bp)
GAAACTCATGAATCACCCCTCTTCCACTAACTTGACCTCATTAGATCGGA6620.1515408175878914No Hit
TGACTGTTAGTGAGGAATGTGAAATTGTCCTCTGATGATAGATCGGAAGA6330.1449023225576061No Hit
TCCCGTAGATCCGAACTTGTAGATCGGAAGAGCACACGTCTGAACTCCAG6020.1378060002838529Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GAGGTCCGGACTTGGGGAGGGTTATCTACTACTTAGATCGGAAGAGCACA5840.13368555509264135No Hit
ACGATTCTATAGACACCCCTCCTCCAAATACTTGACCTCTTTAGATCGGA5700.1304807643883657No Hit
AAATCAGTTGATCTCCCCCCTCCCCGCTCTTAGACCTCACTAGATCGGAA5430.12430009660154837No Hit
GAGGTCCGGACGGAGGAGGGTTATCCACAGATCGGAAGAGCACACGTCTG5350.12246878762767657Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GAGGTCCGGACGGTCGAGGGTTATCATCCTTAGATCGGAAGAGCACACGT5340.1222398740059426No Hit
GAGGTCCGGACTTGGGGAGGGTTATCTACTACTTACAAATCAGTTAGATC5000.11445681086698745No Hit
CGGGCTGAGACTTGAAGCGGGTAGATCGGAAGAGCACACGTCTGAACTCC5000.11445681086698745Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTGTTTAAATATTCTGTTGATGAACTCAGCAGATCGGAAGAGCACACGTC4970.11377007000178553No Hit
TATTAATGCTGTAGGTGAAAGACAAGATCGGAAGAGCACACGTCTGAACT4920.11262550189311565Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GAGGTCCGGATTTGGGGAGGGTTATCTACTAGATCGGAAGAGCACACGTC4850.11102310654097783No Hit
TGAAAATCTTTGCTCTGAAGTGGAAGATCGGAAGAGCACACGTCTGAACT4790.10964962481057397Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCAGGTCTAGGCTGGTTAGTTTAGATCGGAAGAGCACACGTCTGAACTCC4780.10942071118884Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTGGGGGGGGGTG4700.1075894022149682TruSeq Adapter, Index 1 (97% over 41bp)
CACCCCTCCTCCAAATACTTGACCTCTTAGATCGGAAGAGCACACGTCTG4700.1075894022149682Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTGGAGCCGGCCTGGGCTGAGATCGGAAGAGCACACGTCTGAACTCCAGT4440.10163764804988487Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)