FastQCFastQC Report
Wed 30 Apr 2025
1F12_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1F12_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41248264
Total Bases6.1 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC67

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCCACTTCAGAGCAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA14553243.528206665861138Illumina DpnII expression Sequencing Primer (100% over 25bp)
GGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAAC10822172.6236667802552858No Hit
TCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATCATT10029032.431382324356729Illumina RNA PCR Primer (100% over 50bp)
AGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAAC9548922.3149871228520067No Hit
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA6348421.5390756808577446Illumina RNA PCR Primer (100% over 45bp)
AAACTAACCAGCCTAGACCTGAGATCGTCGGACTGTAGAACTCTGAACGT3602330.8733288751255083Illumina DpnII expression Sequencing Primer (96% over 28bp)
GGCGAGAATTCTACCACTGAACCACAGTGCGATCGTCGGACTGTAGAACT2329270.5646952802668254No Hit
GCTGAGTTCATCAACAGAATATTTAAACAAGATCGTCGGACTGTAGAACT2327680.5643098094988919No Hit
AGCGAGAATTCTACCACTGAACCACAGTGCGATCGTCGGACTGTAGAACT2095300.5079728931137563No Hit
AGTAGATAACCCTCCCCAAGTCCGGACCTCGATCGTCGGACTGTAGAACT2042730.49522811432742964No Hit
AAGAATGGCGCAAGAATCCAAAGTGGTCAGATCGTCGGACTGTAGAACTC1703660.41302586697951704Illumina DpnII expression Sequencing Primer (100% over 21bp)
AGGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAA1388950.3367293227176785No Hit
ACTGAGTTCATCAACAGAATATTTAAACAAGATCGTCGGACTGTAGAACT1383180.33533047596863713No Hit
ACAAGTTCGGATCTACGGGAGATCGTCGGACTGTAGAACTCTGAACGTGT1318170.31956981268351076Illumina DpnII expression Sequencing Primer (96% over 30bp)
TCCGACACTCAGACAGACATGCTCCTGGGAGAACCCAAGAGCGCCATTTG1081850.262277704584125No Hit
AATCTCTTTCAGGTGCAGGAATAGACAGCAGATCGTCGGACTGTAGAACT1014900.24604671847523085No Hit
AGCAGAGATTGGTGAGCTGAGAGATCGTCGGACTGTAGAACTCTGAACGT983580.23845367165027842Illumina DpnII expression Sequencing Primer (96% over 28bp)
AAGCCTACAACACGGGGTATTCCCAGGCGGTCACCGATCGTCGGACTGTA953770.2312267008376401No Hit
TGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAAC899590.21809160259447527No Hit
AATGAGGTCAATGAAGGCGGGGAGGGGCGTACCGAAGGATCGTCGGACTG877990.21285501857726666No Hit
AGCGCCGAATCCTAACCACTAGACCACCTGGGAGATCGTCGGACTGTAGA828140.20076966148199596No Hit
GCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAACT801590.19433302696084373No Hit
GAGGTCAAGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGG786210.19060438519303502Illumina RNA PCR Primer (100% over 37bp)
AAACTAACCAGCCTAGACTTGAGATCGTCGGACTGTAGAACTCTGAACGT774340.18772668832802272Illumina DpnII expression Sequencing Primer (96% over 28bp)
GGAAATACTAACCACTATACTACAGCGGAGATCGTCGGACTGTAGAACTC712450.1727224205120487Illumina DpnII expression Sequencing Primer (100% over 21bp)
AAGGATGATAACCCTCGACCGTCCGGACCTCGATCGTCGGACTGTAGAAC661560.1603849315937272No Hit
ACAAATCTTAGAACAAAGGCTTAATCTCAGAAGATCGTAGCAACGATCGT651040.15783452122979041No Hit
AAGTTCTTGGAGCCAACTTATTATGGACTTGTAGATCGTCGGACTGTAGA647060.1568696321377307No Hit
AGAATCGTGGATAACCCTCCTCCGTCCGGACCTCGATCGTCGGACTGTAG628530.1523773218674124No Hit
ACGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAACT604000.1464304049256473No Hit
ACAAATCTTAGAACAAAGGCTTAATCTCAGAAGATCGTAGCAAGATCGTC603600.14633343114755082No Hit
AGTAGATAACCCTCCCCAAATCCGGACCTCGATCGTCGGACTGTAGAACT601330.14578310495685345No Hit
AACACTACTTAGACCAACGACGTTTCCAGATCGTCGGACTGTAGAACTCT597040.14474306118676897Illumina DpnII expression Sequencing Primer (100% over 22bp)
AGCCTTCATAAATTCCATCATCAACACTGATTGATCGTCGGACTGTAGAA590320.14311390171474853No Hit
AAAGAGGTCAAGTATTTGGAGGAGGGGTGTCTATAGAATCGATCGTCGGA584980.14181930177716084No Hit
AATGGAAGCCATACAGGTTGTCACGTTGAAGATCGTCGGACTGTAGAACT572790.13886402588967137No Hit
ACAAACAGATCATAGATCCAGATCCACATAGATCGTCGGACTGTAGAACT562580.13638877020375936No Hit
GAAATACTAACCACTATACTACAGCGGAGATCGTCGGACTGTAGAACTCT548640.13300923403709788Illumina DpnII expression Sequencing Primer (100% over 22bp)
ATCCTCTTCCGGGTGCAGGAATAGACAGCAGATCGTCGGACTGTAGAACT541170.1311982487311466No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG536130.12997637912713125No Hit
AGCGCCGAATCCTAACCACTAGACCACTTGGGAGATCGTCGGACTGTAGA525500.12739930097421798No Hit
GAACTAACCAGCCTAGACCTGAGATCGTCGGACTGTAGAACTCTGAACGT518780.12577014150219754Illumina DpnII expression Sequencing Primer (96% over 28bp)
AACTAACCAGCCTAGACCTGAGATCGTCGGACTGTAGAACTCTGAACGTG518740.12576044412438786Illumina DpnII expression Sequencing Primer (96% over 29bp)
GACGTAATAACAAATCGGAATATCAGGAGATCGTCGGACTGTAGAACTCT478450.11599276032562243Illumina DpnII expression Sequencing Primer (100% over 22bp)
AGTAGTAGATAACCCTCCCCAAGTCCGGACCTCGATCGTCGGACTGTAGA469580.11384236679633354No Hit
GAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAACTCT456950.11078041975293795Illumina DpnII expression Sequencing Primer (100% over 22bp)
AGTCTCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAGA454200.11011372502852483No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)