FastQCFastQC Report
Wed 30 Apr 2025
1F12_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1F12_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41248264
Total Bases6.1 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC66

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAAAATCTTTGCTCTGAAGTGGAAAGATCGGAAGAGCACACGTCTGAAC14050743.406383357127466Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCACTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT11076392.6852984649244878No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGT9882192.3957832504175207No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATCTGGGGGG4680781.1347823025958135TruSeq Adapter, Index 7 (97% over 36bp)
TCAGGTCTAGGCTGGTTAGTTTAGATCGGAAGAGCACACGTCTGAACTCC3630970.8802721976372145Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCACTGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTC2374250.5755999816137717No Hit
TTGTTTAAATATTCTGTTGATGAACTCAGCAGATCGGAAGAGCACACGTC2314450.5611024017883516No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGTC2160460.5237699215656688No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATCTGGGGGGGGGGG2120670.5141234549895239TruSeq Adapter, Index 7 (96% over 32bp)
GAGGTCCGGACTTGGGGAGGGTTATCTACTAGATCGGAAGAGCACACGTC2067130.5011435147913134No Hit
TGACCACTTTGGATTCTTGCGCCATTCTTAGATCGGAAGAGCACACGTCT1724030.41796425662907905Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCACTGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACG1423780.3451733144454273No Hit
TTGTTTAAATATTCTGTTGATGAACTCAGTAGATCGGAAGAGCACACGTC1393080.33773057697652437No Hit
TCCCGTAGATCCGAACTTGTAGATCGGAAGAGCACACGTCTGAACTCCAG1330970.32267297358259733Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AACTTTTGACGGTGGATCTCTTGGCTCACGTATCGATGAAGAACGCAGCC1222480.29637126061838626No Hit
TGCTGTCTATTCCTGCACCTGAAAGAGATTAGATCGGAAGAGCACACGTC1030280.24977536024303956No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATCTGGGGTG1002510.24304295569869314TruSeq Adapter, Index 7 (97% over 36bp)
TCTCAGCTCACCAATCTCTGCTAGATCGGAAGAGCACACGTCTGAACTCC996470.24157865164943668Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGTGACCGCCTGGGAATACCCCGTGTTGTAGGCTTAGATCGGAAGAGCAC961480.23309587041044927No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCAAGATCGGAAGAGCACACGT901140.21846737598459903No Hit
CTTCGGTACGCCCCTCCCCGCCTTCATTGACCTCATTAGATCGGAAGAGC892140.2162854659774288No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATCTGGGGGGGGGGT872290.21147314223939218TruSeq Adapter, Index 7 (96% over 32bp)
TCCCAGGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACAC850310.20614443313299197No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTC808610.19603491676643653No Hit
TCAAGTCTAGGCTGGTTAGTTTAGATCGGAAGAGCACACGTCTGAACTCC781550.18947464067821132Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTGACCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGAT780500.18922008451070815TruSeq Adapter, Index 7 (97% over 36bp)
TCCGCTGTAGTATAGTGGTTAGTATTTCCAGATCGGAAGAGCACACGTCT703220.170484750582473Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GAGGTCCGGACGGTCGAGGGTTATCATCCTTAGATCGGAAGAGCACACGT670780.1626201771788505No Hit
TACAAGTCCATAATAAGTTGGCTCCAAGAACTTAGATCGGAAGAGCACAC666250.16152194914190812No Hit
GTTGCTACGATCTTCTGAGATTAAGCCTTTGTTCTAAGATTTGTAGATCG655590.1589375979556376No Hit
GAGGTCCGGACGGAGGAGGGTTATCCACGATTCTAGATCGGAAGAGCACA639830.15511683109863728No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGTAGATCGGAAGAGCACACGTC617550.1497153916586647No Hit
GAGGTCCGGATTTGGGGAGGGTTATCTACTAGATCGGAAGAGCACACGTC609670.14780500823016454No Hit
TTGCTACGATCTTCTGAGATTAAGCCTTTGTTCTAAGATTTGTAGATCGG609080.14766197190747227No Hit
AATCAGTGTTGATGATGGAATTTATGAAGGCTAGATCGGAAGAGCACACG601990.1459431116907126No Hit
TGGAAACGTCGTTGGTCTAAGTAGTGTTAGATCGGAAGAGCACACGTCTG600950.14569097986766183Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GATTCTATAGACACCCCTCCTCCAAATACTTGACCTCTTTAGATCGGAAG590280.14310420433693888No Hit
TTCAACGTGACAACCTGTATGGCTTCCATTAGATCGGAAGAGCACACGTC588400.14264842757988555No Hit
TATGTGGATCTGGATCTATGATCTGTTTGTAGATCGGAAGAGCACACGTC569150.13798156450899365No Hit
TGCTGTCTATTCCTGCACCCGGAAGAGGATAGATCGGAAGAGCACACGTC547010.13261406589135485No Hit
TCCCAAGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACAC540760.13109885060859772No Hit
TCAGGTCTAGGCTGGTTAGTTCAGATCGGAAGAGCACACGTCTGAACTCC539750.13085399181890417Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCGCTGTAGTATAGTGGTTAGTATTTCAGATCGGAAGAGCACACGTCTG536480.13006123118296564Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCAGGTCTAGGCTGGTTAGTTAGATCGGAAGAGCACACGTCTGAACTCCA521390.1264028954042769Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCCTGATATTCCGATTTGTTATTACGTCAGATCGGAAGAGCACACGTCTG481880.11682431047279954Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GAGGTCCGGACTTGGGGAGGGTTATCTACTACTAGATCGGAAGAGCACAC473370.11476119334379745No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTAGATCGGAAGAGCACAC465170.11277323089282011No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCAGATCGGAAGAGCACACGTCTG461070.11177924966733145Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATCTGGGGGGGCGTT423670.10271220141531288TruSeq Adapter, Index 7 (96% over 32bp)
CACCCCTCTTCCACTAACTTGACCTCATAGATCGGAAGAGCACACGTCTG422790.10249885910350069Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATCTGGGGGGGCGGT419830.10178125314558692TruSeq Adapter, Index 7 (96% over 32bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)