FastQCFastQC Report
Wed 30 Apr 2025
1F10_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1F10_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6281555
Total Bases942.2 Mbp
Sequences flagged as poor quality0
Sequence length150
%GC68

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATCATT3185715.071530855019179Illumina RNA PCR Primer (100% over 50bp)
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA1950003.1043268744761447Illumina RNA PCR Primer (100% over 45bp)
TTCCACTTCAGAGCAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA1304182.0762056528996404Illumina DpnII expression Sequencing Primer (100% over 25bp)
AGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAAC1202041.9136026031770794No Hit
GGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAAC1198131.90737802980313No Hit
AGTAGATAACCCTCCCCAAGTCCGGACCTCGATCGTCGGACTGTAGAACT603280.9603991368379327No Hit
AAAGAGGTCAAGTATTTGGAGGAGGGGTGTCTATAGAATCGATCGTCGGA313950.49979662679065934No Hit
AAGCCTACAACACGGGGTATTCCCAGGCGGTCACCGATCGTCGGACTGTA291250.46365907804675754No Hit
ACAAATCTTAGAACAAAGGCTTAATCTCAGAAGATCGTAGCAAGATCGTC264310.42077160830399474No Hit
AAACTAACCAGCCTAGACCTGAGATCGTCGGACTGTAGAACTCTGAACGT253570.40367393105688Illumina DpnII expression Sequencing Primer (96% over 28bp)
GGCGAGAATTCTACCACTGAACCACAGTGCGATCGTCGGACTGTAGAACT249660.3974493576829304No Hit
AGCGAGAATTCTACCACTGAACCACAGTGCGATCGTCGGACTGTAGAACT249080.3965260194330862No Hit
AAGAATGGCGCAAGAATCCAAAGTGGTCAGATCGTCGGACTGTAGAACTC240660.3831216951853482Illumina DpnII expression Sequencing Primer (100% over 21bp)
ACAAATCTTAGAACAAAGGCTTAATCTCAGAAGATCGTAGCAACGATCGT223280.35545338694001727No Hit
AAGGATGATAACCCTCGACCGTCCGGACCTCGATCGTCGGACTGTAGAAC215970.343816141066981No Hit
AGTAGATAACCCTCCCCAAATCCGGACCTCGATCGTCGGACTGTAGAACT202690.3226748790705486No Hit
AGGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAA201890.32140130907076353No Hit
TCCGACACTCAGACAGACATGCTCCTGGGAGAACCCAAGAGCGCCATTTG200110.3185676158212417No Hit
AGAATCGTGGATAACCCTCCTCCGTCCGGACCTCGATCGTCGGACTGTAG195450.31114907057249364No Hit
AATGAGGTCAATGAAGGCGGGGAGGGGCGTACCGAAGGATCGTCGGACTG186140.2963278996999947No Hit
GTGGATAACCCTCCTCCGTCCGGACCTCGATCGTCGGACTGTAGAACTCT154860.24653131270839784Illumina DpnII expression Sequencing Primer (100% over 22bp)
AGTAGTAGATAACCCTCCCCAAGTCCGGACCTCGATCGTCGGACTGTAGA154330.2456875725835402No Hit
GAGGTCAAGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGG152300.24245588870908558Illumina RNA PCR Primer (100% over 37bp)
AACACTACTTAGACCAACGACGTTTCCAGATCGTCGGACTGTAGAACTCT141040.22453039096211053Illumina DpnII expression Sequencing Primer (100% over 22bp)
AAAGAGGTCAAGTATTTGGAGGAGGGGTGTCTATAGAATCGTGGGATCGT134880.21472390196376534No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG131200.20886547996475396No Hit
AGATTAAGAGTCTCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGG120680.19211803446758008No Hit
ACAAGTTCGGATCTACGGGAGATCGTCGGACTGTAGAACTCTGAACGTGT119870.19082854484279768Illumina DpnII expression Sequencing Primer (96% over 30bp)
GGTAGATAACCCTCCCCAAGTCCGGACCTCGATCGTCGGACTGTAGAACT109730.1746860450955217No Hit
CGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATCATTA108810.17322143959576888Illumina RNA PCR Primer (100% over 49bp)
AATCGTGGATAACCCTCCTCCGTCCGGACCTCGATCGTCGGACTGTAGAA107590.17127924534609665No Hit
TGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAAC106980.1703081482212605No Hit
AAATGAGGTCAATGAAGGCGGGGAGGGGCGTACCGAAGGATCGTCGGACT97910.15586904834869708No Hit
ATGAGGTCAAGTTAGTGGAAGAGGGGTGGATCGTCGGACTGTAGAACTCT87340.13904200472653666Illumina DpnII expression Sequencing Primer (100% over 22bp)
GAATCGTGGATAACCCTCCTCCGTCCGGACCTCGATCGTCGGACTGTAGA85930.13679733760191545No Hit
AAAGAGGTCAAGTATTTGGAGGAGGGGTGTCTATAGAATCGGATCGTCGG84950.13523721435217873No Hit
ACTCTGGGACAGATACAAAGTTGACTGGAAGATCGTCGGACTGTAGAACT79040.1258287159787664No Hit
AAGTAGTAGATAACCCTCCCCAAGTCCGGACCTCGATCGTCGGACTGTAG77640.1235999684791425No Hit
ACTTAGAAGAAAACGTTCGTGGATCATGAAGATCGTCGGACTGTAGAACT75120.1195882229798195No Hit
CGTGGATAACCCTCCTCCGTCCGGACCTCGATCGTCGGACTGTAGAACTC74980.11936534822985709Illumina DpnII expression Sequencing Primer (100% over 21bp)
AAGAGGTCAAGTATTTGGAGGAGGGGTGTCTATAGAATCGATCGTCGGAC72810.11591078960544006No Hit
AAAGAGGTCAAGTATTTGGAGGAGGGGTGTCTATAGAATCGTGATCGTCG71690.11412779160574094No Hit
AAGTTCTTGGAGCCAACTTATTATGGACTTGTAGATCGTCGGACTGTAGA70760.11264726648099077No Hit
AAAATGAGGTCAATGAAGGCGGGGAGGGGCGTACCGAAGGATCGTCGGAC70570.1123447936060418No Hit
AGTCTCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAGA68010.10826936960672955No Hit
ATGGATAACCCTCCTCCGTCCGGACCTCGATCGTCGGACTGTAGAACTCT65490.10425762410740654Illumina DpnII expression Sequencing Primer (100% over 22bp)
ATAACCCTCCTCCGTCCGGACCTCGATCGTCGGACTGTAGAACTCTGAAC63950.10180600185782024Illumina DpnII expression Sequencing Primer (100% over 26bp)
AATGGAAGCCATACAGGTTGTCACGTTGAAGATCGTCGGACTGTAGAACT63680.10137617198289278No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)