FastQCFastQC Report
Wed 30 Apr 2025
1F10_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1F10_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6281555
Total Bases942.2 Mbp
Sequences flagged as poor quality0
Sequence length150
%GC65

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAGAGATCTGGGGGG1424842.268291848117226TruSeq Adapter, Index 3 (97% over 37bp)
GCACTGGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGT1229111.9566970280448075No Hit
TGAAAATCTTTGCTCTGAAGTGGAAAGATCGGAAGAGCACACGTCTGAAC1214921.9341070801736193Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCACTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT1205821.919620221426064No Hit
GAGGTCCGGACTTGGGGAGGGTTATCTACTAGATCGGAAGAGCACACGTC600990.9567535427135478No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACGTAGAGATCTGGGGGGGGGGG371570.5915255060251801TruSeq Adapter, Index 3 (96% over 33bp)
GATTCTATAGACACCCCTCCTCCAAATACTTGACCTCTTTAGATCGGAAG314380.5004811706655438No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAGAGATCTGGGGTG297580.473736200670057TruSeq Adapter, Index 3 (97% over 37bp)
GGTGACCGCCTGGGAATACCCCGTGTTGTAGGCTTAGATCGGAAGAGCAC289520.46090498292222226No Hit
TTGCTACGATCTTCTGAGATTAAGCCTTTGTTCTAAGATTTGTAGATCGG265220.42222029417875034No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGTC254500.40515445618163015No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTC249660.3974493576829304No Hit
TCAGGTCTAGGCTGGTTAGTTTAGATCGGAAGAGCACACGTCTGAACTCC249050.3964782605580943Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGACCACTTTGGATTCTTGCGCCATTCTTAGATCGGAAGAGCACACGTCT240380.38267594568542346Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AACTTTTGACGGTGGATCTCTTGGCTCACGTATCGATGAAGAACGCAGCC230450.3668677580630911No Hit
GTTGCTACGATCTTCTGAGATTAAGCCTTTGTTCTAAGATTTGTAGATCG223870.35639264481485877No Hit
GAGGTCCGGACGGTCGAGGGTTATCATCCTTAGATCGGAAGAGCACACGT215400.34290872244213416No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACG204070.3248717873201779No Hit
GAGGTCCGGATTTGGGGAGGGTTATCTACTAGATCGGAAGAGCACACGTC202010.32159234457073127No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACGTAGAGATCTGGGGGGGCGTT201540.32084412219585756TruSeq Adapter, Index 3 (96% over 33bp)
GAGGTCCGGACGGAGGAGGGTTATCCACGATTCTAGATCGGAAGAGCACA196900.31345741619710404No Hit
CTTCGGTACGCCCCTCCCCGCCTTCATTGACCTCATTAGATCGGAAGAGC186050.2961846230750188No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACGTAGAGATCTGGGGGGGGGGT176380.28079034570261663TruSeq Adapter, Index 3 (96% over 33bp)
GAGGTCCGGACTTGGGGAGGGTTATCTACTACTAGATCGGAAGAGCACAC153830.24489159133367455No Hit
GAGGTCCGGACGGAGGAGGGTTATCCACAGATCGGAAGAGCACACGTCTG151740.241564389709236Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTGACCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAGAGAT146700.23354089871058997TruSeq Adapter, Index 3 (97% over 37bp)
GGAAGAGCACACGTCTGAACTCCAGTCACGTAGAGATCTGGGGGGGGGTT143250.2280486280865168TruSeq Adapter, Index 3 (96% over 33bp)
CCACGATTCTATAGACACCCCTCCTCCAAATACTTGACCTCTTTAGATCG140280.2233204994623147No Hit
TGGAAACGTCGTTGGTCTAAGTAGTGTTAGATCGGAAGAGCACACGTCTG138430.22037536883781164Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGAAGAGCACACGTCTGAACTCCAGTCACGTAGAGATCTGGGGGGGCGTC130900.20838789121483456TruSeq Adapter, Index 3 (96% over 33bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCTTAATCTAGATCGGAA121590.1935667203423356No Hit
TCCCGTAGATCCGAACTTGTAGATCGGAAGAGCACACGTCTGAACTCCAG118170.18812220859325438Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GGAAGAGCACACGTCTGAACTCCAGTCACGTAGAGATCTGGGGGGGCGGT114470.18223194734424836TruSeq Adapter, Index 3 (96% over 33bp)
GAGGTCCGGACTTGGGGAGGGTTATCTACCAGATCGGAAGAGCACACGTC111870.17809284484494683No Hit
GAGGTCCGGACGGAGGAGGGTTATCCACGATTAGATCGGAAGAGCACACG108320.17244137797090053No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCAAGATCGGAAGAGCACACGT104810.16685358959684346No Hit
CTTCGGTACGCCCCTCCCCGCCTTCATTGACCTCATTTAGATCGGAAGAG98400.15664910997356546No Hit
CACCCCTCTTCCACTAACTTGACCTCATAGATCGGAAGAGCACACGTCTG88560.14098419897620892Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGAAGAGCACACGTCTGAACTCCAGTCACGTAGAGATCTGGGGGGGGGTG87240.1388828084765635TruSeq Adapter, Index 3 (96% over 33bp)
CGATTCTATAGACACCCCTCCTCCAAATACTTGACCTCTTTAGATCGGAA85920.13678141797691812No Hit
GAGGTCCGGACGGAGGAGGGTTATCCACGATTCAGATCGGAAGAGCACAC84740.13490290222723514No Hit
TTCCAGTCAACTTTGTATCTGTCCCAGAGTAGATCGGAAGAGCACACGTC78490.12495313660391416No Hit
GAGGTCCGGACTTGGGGAGGGTTATCTACTACTTAGATCGGAAGAGCACA77810.12387060210409684No Hit
TTCATGATCCACGAACGTTTTCTTCTAAGTAGATCGGAAGAGCACACGTC74800.11907879497990545No Hit
GATTCTATAGACACCCCTCCTCCAAATACTTGACCTCTTAGATCGGAAGA72910.1160699858554132No Hit
ACGATTCTATAGACACCCCTCCTCCAAATACTTGACCTCTTTAGATCGGA72590.11556055785549915No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACGTAGAGATCTGGGGGGTCGTT72370.11521032610555826TruSeq Adapter, Index 3 (96% over 33bp)
TACAAGTCCATAATAAGTTGGCTCCAAGAACTTAGATCGGAAGAGCACAC72000.11462129998065765No Hit
GAGGTCCGGACGGAGGAGGGTTATCCACGAGATCGGAAGAGCACACGTCT71300.11350692623084571Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTTCGGTACGCCCCTCCCCGCCTTCATTGACCTCATTTTAGATCGGAAGA70460.11216967773107138No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTAGATCGGAAGAGCACAC68870.10963845735649852No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACGTAGAGATCTGGGGTGGCGTT68060.10834896773171611TruSeq Adapter, Index 3 (96% over 33bp)
GGAAGAGCACACGTCTGAACTCCAGTCACGTAGAGATCTGGGGGGGCGGG65920.104942167982291TruSeq Adapter, Index 3 (96% over 33bp)
GAGGTCCGGACGGAGGAGGGTTATCCATAGATCGGAAGAGCACACGTCTG65860.10484665023230712Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTCAACGTGACAACCTGTATGGCTTCCATTAGATCGGAAGAGCACACGTC63780.10153536823286592No Hit
GAGGTCCGGACGGAGGAGGGTTATAGATCGGAAGAGCACACGTCTGAACT63310.10078714585799217Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)