FastQCFastQC Report
Wed 30 Apr 2025
1E7_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1E7_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences271172
Total Bases40.6 Mbp
Sequences flagged as poor quality0
Sequence length150
%GC74

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA11380541.96782853686959Illumina RNA PCR Primer (100% over 45bp)
TCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATCATT3373212.439337394716269Illumina RNA PCR Primer (100% over 50bp)
TTCCACTTCAGAGCAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA130234.802486982431815Illumina DpnII expression Sequencing Primer (100% over 25bp)
GAGGTCAAGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGG14960.5516793769268213Illumina RNA PCR Primer (100% over 37bp)
ACAAATCTTAGAACAAAGGCTTAATCTCAGAAGATCGTAGCAACGATCGT9760.35991916569557325No Hit
ACAAATCTTAGAACAAAGGCTTAATCTCAGAAGATCGTAGCAAGATCGTC9690.3573377782366911No Hit
GGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGT7860.2898529346687711Illumina RNA PCR Primer (100% over 44bp)
GCTTTAAGAAAAGACACACCTCACAATTATACGATCGTCGGACTGTAGAA7030.2592450547991681No Hit
CTGGCTGCGTTCTTCATCGATACGTGAGCCAAGAGATCCACCGTCAAAAG5490.20245453070376No Hit
TACCACTTCAGAGCAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA5350.1972917557859956Illumina DpnII expression Sequencing Primer (100% over 25bp)
CGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATCATTA5170.19065390232029855Illumina RNA PCR Primer (100% over 49bp)
TTTTTTTTTCCTTCACGAGTAAACATTCTAGATCGTCGGACTGTAGAACT4650.17147788119717375No Hit
TGTCTTTCACCTACAGCATTAATAGATCGTCGGACTGTAGAACTCTGAAC4610.17000280264924109Illumina DpnII expression Sequencing Primer (100% over 26bp)
TTCCCACTTCAGAGCAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGA4290.15820217426577965Illumina DpnII expression Sequencing Primer (100% over 24bp)
AGTAGATAACCCTCCCCAAGTCCGGACCTCGATCGTCGGACTGTAGAACT3770.13902615314265485No Hit
TCCACTTCAGAGCAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAAC3700.13644476568377265Illumina DpnII expression Sequencing Primer (100% over 26bp)
TCCATGAATTAATGAGCCGATCGATCGTCGGACTGTAGAACTCTGAACGT3300.12169398020444588Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGAATCGTGGATAACCCTCCTCCGTCCGGACCTCGATCGTCGGACTGTAG3200.1180062838346142No Hit
GATCGTCGGACTGTAAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTAT2950.10878704291003495Illumina RNA PCR Primer (100% over 35bp)
AAAGTAAACGTCACAGATCCTGAGCACACGATCGTCGGACTGTAGAACTC2900.10694319472511911Illumina DpnII expression Sequencing Primer (100% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)