FastQCFastQC Report
Wed 30 Apr 2025
1D7_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1D7_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10826531
Total Bases1.6 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC67

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCCACTTCAGAGCAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA183117016.913727952194474Illumina DpnII expression Sequencing Primer (100% over 25bp)
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA3143082.9031275114808244Illumina RNA PCR Primer (100% over 45bp)
GCTGAGTTCATCAACAGAATATTTAAACAAGATCGTCGGACTGTAGAACT1895921.7511795791283469No Hit
GGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAAC1527561.4109413255270777No Hit
ACAAATCTTAGAACAAAGGCTTAATCTCAGAAGATCGTAGCAAGATCGTC1281731.1838787511900164No Hit
ACAAATCTTAGAACAAAGGCTTAATCTCAGAAGATCGTAGCAACGATCGT1234981.140697791379344No Hit
TTTTTTTGGTCTTTCACCTACAGCATTAATAGATCGTCGGACTGTAGAAC782260.7225398421710518No Hit
GAGGTCAAGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGG485880.44878641182480333Illumina RNA PCR Primer (100% over 37bp)
TCCACTTCAGAGCAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAAC440550.40691704480410207Illumina DpnII expression Sequencing Primer (100% over 26bp)
GCTTTAAGAAAAGACACACCTCACAATTATACGATCGTCGGACTGTAGAA320570.29609669062047667No Hit
TGTCTTTCACCTACAGCATTAATAGATCGTCGGACTGTAGAACTCTGAAC316700.2925221384393579Illumina DpnII expression Sequencing Primer (100% over 26bp)
CTGGCTGCGTTCTTCATCGATACGTGAGCCAAGAGATCCACCGTCAAAAG292800.27044673866449004No Hit
ACAAATCTTAGAACAAAGGCTTAATCTCAGAAGATCGTAGCGATCGTCGG266420.24608066979164422No Hit
AGGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAA250170.2310712452585228No Hit
AAGCCTACAACACGGGGTATTCCCAGGCGGTCACCGATCGTCGGACTGTA240960.2225643652615967No Hit
TCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATCATT229290.21178528930457965Illumina RNA PCR Primer (100% over 50bp)
TTTTTTTTTCCTTCACGAGTAAACATTCTAGATCGTCGGACTGTAGAACT227630.2102520188599654No Hit
CCACTTCAGAGCAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAACG208870.19292421552203565Illumina Small RNA Adapter 1 (100% over 26bp)
TTTTTTTTGGTCTTTCACCTACAGCATTAATAGATCGTCGGACTGTAGAA206150.19041186876941468No Hit
TTTTCCTCCAATCCTCACGGCATAAACAAGATCGTCGGACTGTAGAACTC201890.186477090399501Illumina DpnII expression Sequencing Primer (100% over 21bp)
GTGGATAACCCTCCTCCGTCCGGACCTCGATCGTCGGACTGTAGAACTCT187640.17331497965507142Illumina DpnII expression Sequencing Primer (100% over 22bp)
ACAAATCTTAGAACAAAGGCTTAATCTCAGAAGATCGTAGCAGATCGTCG184790.1706825575061855No Hit
GGCGAGAATTCTACCACTGAACCACAGTGCGATCGTCGGACTGTAGAACT179680.16596267077607776No Hit
CCGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCTGAACGT177540.16398604502217748Illumina DpnII expression Sequencing Primer (96% over 28bp)
ACCCGCTTCAAGTCTCAGCCCGAGGATCGTCGGACTGTAGAACTCTGAAC164040.15151667694850732Illumina DpnII expression Sequencing Primer (100% over 26bp)
TTTTTTGAGCCAAGACGCTATCACTAGATCGTCGGACTGTAGAACTCTGA162990.1505468372094441Illumina DpnII expression Sequencing Primer (100% over 24bp)
GTCTCATGCTCTACCGACTGAGCTAGCCGGGCGATCGTCGGACTGTAGAA158800.14667671482213462No Hit
CCTGATCCTTGTCCATTGTATGAGATCGTCGGACTGTAGAACTCTGAACG157550.1455221437042022Illumina Small RNA Adapter 1 (100% over 26bp)
AATGAGGTCAATGAAGGCGGGGAGGGGCGTACCGAAGGATCGTCGGACTG154820.1430005603826378No Hit
AAACTAACCAGCCTAGACCTGAGATCGTCGGACTGTAGAACTCTGAACGT153830.14208614005723533Illumina DpnII expression Sequencing Primer (96% over 28bp)
AGTAGATAACCCTCCCCAAGTCCGGACCTCGATCGTCGGACTGTAGAACT153240.1415411824895712No Hit
GGCAGAGTTGTGCACATTCATGCTGCCAGAGGTCACGATCGTCGGACTGT139660.1289979218643534No Hit
GCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAACT134790.1244997127888887No Hit
ACAAGTTCGGATCTACGGGAGATCGTCGGACTGTAGAACTCTGAACGTGT134330.12407483061748958Illumina DpnII expression Sequencing Primer (96% over 30bp)
AAGGATGATAACCCTCGACCGTCCGGACCTCGATCGTCGGACTGTAGAAC133500.12330819539518245No Hit
TACCACTTCAGAGCAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA124350.11485673481191713Illumina DpnII expression Sequencing Primer (100% over 25bp)
TCCCACTTCAGAGCAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA121280.11202110814627511Illumina DpnII expression Sequencing Primer (100% over 25bp)
CCAAGACCACAAAGGTACCAGACATCGGAGATCGTCGGACTGTAGAACTC115660.10683015640005095Illumina DpnII expression Sequencing Primer (100% over 21bp)
CCACAAGTTCGGATCTACGGGAGATCGTCGGACTGTAGAACTCTGAACGT115450.10663618845223831Illumina DpnII expression Sequencing Primer (96% over 28bp)
TGGCTCCCCGGCGGGGAATCGAACCCCGGTCTCCCGCGATCGTCGGACTG112940.10431780964743No Hit
GTCTGATGCTCTACCGACTGAGCTAACCGGGCGATCGTCGGACTGTAGAA111690.10316323852949758No Hit
ACAAATCTTAGAACAAAGGCTTAATCTCAGAAGATCGTAGCAACAGATCG111050.10257209811711618No Hit
GGTCTTTCACCTACAGCATTAATAGATCGTCGGACTGTAGAACTCTGAAC110190.10177775318797869Illumina DpnII expression Sequencing Primer (100% over 26bp)
TTTTTTGGTCTTTCACCTACAGCATTAATAGATCGTCGGACTGTAGAACT108530.10024448274336444No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)