FastQCFastQC Report
Wed 30 Apr 2025
1D7_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1D7_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10826531
Total Bases1.6 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC69

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAAAATCTTTGCTCTGAAGTGGAAAGATCGGAAGAGCACACGTCTGAAC179040316.537180746076466Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTGGGGGG2722002.5141940664096376TruSeq Adapter, Index 4 (97% over 45bp)
TTGTTTAAATATTCTGTTGATGAACTCAGCAGATCGGAAGAGCACACGTC1868831.7261577138605155No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT1535281.4180719567514286No Hit
TTGCTACGATCTTCTGAGATTAAGCCTTTGTTCTAAGATTTGTAGATCGG1268361.1715294585126113No Hit
GTTGCTACGATCTTCTGAGATTAAGCCTTTGTTCTAAGATTTGTAGATCG1215751.1229358693010716No Hit
TATTAATGCTGTAGGTGAAAGACCAAAAAAAAGATCGGAAGAGCACACGT774500.7153722646709273No Hit
TTGACCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAAT477840.441360210394262TruSeq Adapter, Index 4 (100% over 41bp)
AACTTTTGACGGTGGATCTCTTGGCTCACGTATCGATGAAGAACGCAGCC465220.4297036603876163No Hit
TGAAAATCTTTGCTCTGAAGTGGAAGATCGGAAGAGCACACGTCTGAACT428500.39578697922723355Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTAGGGGG331930.3065894329402465TruSeq Adapter, Index 4 (97% over 45bp)
GTATAATTGTGAGGTGTGTCTTTTCTTAAAGCAGATCGGAAGAGCACACG314850.2908133731848179No Hit
TATTAATGCTGTAGGTGAAAGACAAGATCGGAAGAGCACACGTCTGAACT309830.2861766155752013Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GCTACGATCTTCTGAGATTAAGCCTTTGTTCTAAGATTTGTAGATCGGAA261900.24190574062920062No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACG249020.230009039830025No Hit
GGTGACCGCCTGGGAATACCCCGTGTTGTAGGCTTAGATCGGAAGAGCAC238270.22007972821580618No Hit
TAGAATGTTTACTCGTGAAGGAAAAAAAAAAGATCGGAAGAGCACACGTC228830.21136040713318052No Hit
TATTAATGCTGTAGGTGAAAGACCAAAAAAAAAGATCGGAAGAGCACACG206410.19065201956194464No Hit
TGAAAATCTTTGCTCTGAAGTGGAGATCGGAAGAGCACACGTCTGAACTC202660.1871883062081474Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TTGTTTATGCCGTGAGGATTGGAGGAAAAAGATCGGAAGAGCACACGTCT197230.18217284927184893Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GAGGTCCGGACGGAGGAGGGTTATCCACAGATCGGAAGAGCACACGTCTG185270.17112591281547154Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGCTACGATCTTCTGAGATTAAGCCTTTGTTCTAAGATTTGTAGATCGGA182960.16899226538953244No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTC179500.1657964125350955No Hit
CTCGGGCTGAGACTTGAAGCGGAGATCGGAAGAGCACACGTCTGAACTCC172790.15959867477403428Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTCGGGCTGAGACTTGAAGCGGGTAGATCGGAAGAGCACACGTCTGAACT161690.14934608324679438Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TAGTGATAGCGTCTTGGCTCAAAAAAAGATCGGAAGAGCACACGTCTGAA160060.1478405225090105Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACAGATCGGAAGAGCACACG158320.14623335951284858No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTGGGGGGGGGGG156520.14457077710302588TruSeq Adapter, Index 4 (97% over 41bp)
TCATACAATGGACAAGGATCAGGAGATCGGAAGAGCACACGTCTGAACTC154390.14260338791806904Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CTTCGGTACGCCCCTCCCCGCCTTCATTGACCTCATTAGATCGGAAGAGC152010.1404050845095257No Hit
GAGGTCCGGACTTGGGGAGGGTTATCTACTAGATCGGAAGAGCACACGTC151280.13973081497665318No Hit
TCAGGTCTAGGCTGGTTAGTTTAGATCGGAAGAGCACACGTCTGAACTCC151180.13963844928721858Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GTGACCTCTGGCAGCATGAATGTGCACAACTCTGCCAGATCGGAAGAGCA139890.12921036295005298No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTC133770.12355758275665585No Hit
GAGGTCCGGACGGTCGAGGGTTATCATCCTTAGATCGGAAGAGCACACGT132630.12250461389710149No Hit
TCCCGTAGATCCGAACTTGTAGATCGGAAGAGCACACGTCTGAACTCCAG131170.1211560748313564Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TGAAAATCTTTGCTCTGAAGTGGTAAGATCGGAAGAGCACACGTCTGAAC121410.11214118354254009Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TGAAAATCTTTGCTCTGAAGTGGGAAGATCGGAAGAGCACACGTCTGAAC117130.10818793203473946Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCCGATGTCTGGTACCTTTGTGGTCTTGGAGATCGGAAGAGCACACGTCT113640.10496436947347217Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCCGTAGATCCGAACTTGTGGAGATCGGAAGAGCACACGTCTGAACTCC112890.10427162680271271Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCGGGAGACCGGGGTTCGATTCCCCGCCGGGGAGCCAAGATCGGAAGAGC111750.10321865794315835No Hit
GCCCGGTTAGCTCAGTCGGTAGAGCATCAGACAGATCGGAAGAGCACACG111430.10292308773696764No Hit
TATTAATGCTGTAGGTGAAAGACCAGATCGGAAGAGCACACGTCTGAACT110640.10219339879043435Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TGTTGCTACGATCTTCTGAGATTAAGCCTTTGTTCTAAGATTTGTAGATC109730.10135287101657954No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)